CD19 Proteins

9269-CD is immobilized at 2 µg/mL to 100 µL/well, recombinant biotinylated human CD81 Fc Chimera binds with an ED₅₀ of 4-20 μg/mL." class="big_lightbox"> 9269-CD is immobilized at 2 µg/mL to 100 µL/well, recombinant biotinylated human CD81 Fc Chimera binds with an ED₅₀ of 4-20 μg/mL." alt="When Recombinant Human CD19 Fc Chimera 9269-CD is immobilized at 2 µg/mL to 100 µL/well, recombinant biotinylated human CD81 Fc Chimera binds with an ED₅₀ of 4-20 μg/mL." class="big_thumb" />

MAB4867) with an ED₅₀ of 25.0-250 ng/mL." class="big_lightbox"> MAB4867) with an ED₅₀ of 25.0-250 ng/mL." alt="Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CD19 Fc Chimera (Catalog # BT9269) binds to Human CD19 Antibody (MAB4867) with an ED₅₀ of 25.0-250 ng/mL." class="big_thumb" />

FC001) then stained with Human CD4 PE-Cy7-conjugated Antibody and rhCD19 Atto 647N-conjugated protein (ATM9269) for 25 min at room temp in the dark. Staining was performed using our staining membrane proteins protocol." class="big_lightbox"> FC001) then stained with Human CD4 PE-Cy7-conjugated Antibody and rhCD19 Atto 647N-conjugated protein (ATM9269) for 25 min at room temp in the dark. Staining was performed using our staining membrane proteins protocol." alt="Human PBMC CD4+ and CD8+ T cells were either (A) transduced or (B) not transduced (negative control) with a Human CD19 CAR and then cultured for 11 days. Cells were washed twice with 2mL 1x PBS then stained with a viability dye for 15-30 min at room temp in the dark. Human block (1-001-1) was added during the viability stain to block Fc receptors. Cells were washed twice with Flow Cytometry Staining Buffer (FC001) then stained with Human CD4 PE-Cy7-conjugated Antibody and rhCD19 Atto 647N-conjugated protein (ATM9269) for 25 min at room temp in the dark. Staining was performed using our staining membrane proteins protocol." class="big_thumb" />

ATM9269) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-92 kDa and 154-184 kDa, respectively." class="big_lightbox"> ATM9269) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-92 kDa and 154-184 kDa, respectively." class="big_thumb" />

1-001-A) was added during the viability stain to block Fc receptors. Cells were washed twice with Flow Cytometry Staining Buffer (FC001) then stained with Human CD4 PE-Cy7-conjugated Antibody and rhCD19 Atto 488-conjugated protein (ATJ9269) for 25 min at room temp in the dark. Staining was performed using our staining membrane proteins protocol." class="big_lightbox"> 1-001-A) was added during the viability stain to block Fc receptors. Cells were washed twice with Flow Cytometry Staining Buffer (FC001) then stained with Human CD4 PE-Cy7-conjugated Antibody and rhCD19 Atto 488-conjugated protein (ATJ9269) for 25 min at room temp in the dark. Staining was performed using our staining membrane proteins protocol." alt="Human PBMC CD4+ and CD8+ T cells were either (A) transduced or (B) not transduced (negative control) with a Human CD19 CAR and then cultured for 11 days. Cells were washed twice with 2mL 1x PBS then stained with a viability dye for 15-30 min at room temp in the dark. Human block (1-001-A) was added during the viability stain to block Fc receptors. Cells were washed twice with Flow Cytometry Staining Buffer (FC001) then stained with Human CD4 PE-Cy7-conjugated Antibody and rhCD19 Atto 488-conjugated protein (ATJ9269) for 25 min at room temp in the dark. Staining was performed using our staining membrane proteins protocol." class="big_thumb" />

ATJ9269) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-92 kD and 154-184 kDa, respectively." class="big_lightbox"> ATJ9269) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-92 kD and 154-184 kDa, respectively." class="big_thumb" />