CD19 Antibody (CB19) [Alexa Fluor® 700]

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Flow Cytometry: CD19 Antibody (CB19) [Alexa Fluor (R) 700] [NBP2-25196AF700] - Flow cytometry on human PBMC. Image from verified customer review.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
CB19
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

CD19 Antibody (CB19) [Alexa Fluor® 700] Summary

Immunogen
Intact normal human B cell cells were used as immunogen to generate the CB19 clone.
Epitope
The exact CD19 epitope recognized by the antibody has not been mapped and is unknown.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD19
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
Reviewed Applications
Read 1 Review rated 4
using
NBP2-25196AF700 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27532872).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD19 Antibody (CB19) [Alexa Fluor® 700]

  • B4
  • B-lymphocyte antigen CD19
  • B-lymphocyte surface antigen B4
  • CD19 antigen
  • CD19 molecule
  • CD19
  • CVID3
  • Differentiation antigen CD19
  • Leu-12
  • MGC12802
  • T-cell surface antigen Leu-12

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC
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Review for CD19 Antibody (NBP2-25196AF700) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NBP2-25196AF700:
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Flow
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Images Ratings Applications Species Date Details
Flow Cytometry CD19 NBP2-25196AF700
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reviewed by:
Anon ymous
Flow Human 09/17/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman
LotAB11308A-AF700

Primary Anitbody

Dilution Ratio1/100, 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS

Details

Detection Notesflow cytometry
Wash Description2% human serum, 0.5 mM EDTA in DPBS, washed 2x after antibody staining

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD19 Antibody (NBP2-25196AF700) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CD19 Products

Bioinformatics Tool for CD19 Antibody (NBP2-25196AF700)

Discover related pathways, diseases and genes to CD19 Antibody (NBP2-25196AF700). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CD19 Antibody (NBP2-25196AF700)

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Pathways for CD19 Antibody (NBP2-25196AF700)

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PTMs for CD19 Antibody (NBP2-25196AF700)

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Research Areas for CD19 Antibody (NBP2-25196AF700)

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Blogs on CD19.

CD19: An Undoubted Biomarker for B Cells
CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t...  Read full blog post.

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Recent Reviews

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Anon ymous
09/17/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CD19
Entrez