CD11c Antibody (ICRF 3.9)

• Reactivity: Hu
• Applications: Flow, IP, CyTOF-ready
• Clone: ICRF 3.9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD11c Antibody (ICRF 3.9) Summary

• Immunogen: Rheumatoid synovial fluid cells and human monocyte-derived fibronectin
• Marker: Dendritic Cell Marker
• Specificity: Detects human CD11c.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAX
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD11c Antibody (ICRF 3.9)

• 95 alpha chain
• 95
• 95, alpha subunit
• CD11 antigen-like family member C
• CD11c antigen
• CD11c
• CD11Cleu M5, alpha subunit
• Integrin alpha X
• integrin alpha-X
• integrin, alpha X (antigen CD11C (p150), alpha polypeptide)
• integrin, alpha X (complement component 3 receptor 4 subunit)
• ITGAX
• Leu M5
• Leukocyte adhesion glycoprotein p150
• Leukocyte adhesion receptor p150
• leukocyte surface antigen p150
• myeloid membrane antigen, alpha subunit
• p150 95 integrin alpha chain
• p150,95 alpha
• SLEB6

Background

CD11c, also known as Integrin alpha X, is an approximately 150 kDa type I transmembrane glycoprotein that heterodimerizes with Integrin beta 2, also known as CD18. The CD11c/CD18 complex, also called CR4 (complement receptor type 4) is expressed on macrophages, dendritic cells and hairy cell leukemias, with lower amounts on other myeloid cells and activated B, NK and some cytotoxic T cells. It binds fibrinogen and has been reported to be a receptor for complement component iC3b (1-3). The human CD11c extracellular domain (amino acids 20-1107) shares 70 - 76% amino acid sequence identity with mouse, rat and canine CD11c.

1. Hogg, N. et al. (1986) Eur. J. Immunol. 16(3):240.
2. Knapp, W.B. et al. eds. (1989) Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York.
3. Stacker, S.A. and T.A. Springer, J. Immunol. (1991) 146(2):648.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD11c Antibody (MAB1777)(6)

Publications using MAB1777

• He S, Wu Q, Gong C, Luo S, Zhang S, Li M, Lu L, Wei Y, Yang L Enhanced efficacy of combination therapy with adenoassociated virus-delivered pigment epithelium-derived factor and cisplatin in a mouse model of Lewis lung carcinoma. Mol Med Rep, 2014-04-04;9(6):2069-76. 2014-04-04 [PMID: 24714917] (IHC-Fr, Western Blot, Mouse)
• Du M, Guo P, Piao H, Wang S, Sun C, Jin L, Tao Y, Li Y, Zhang D, Zhu R, Fu Q, Li D Embryonic trophoblasts induce decidual regulatory T cell differentiation and maternal-fetal tolerance through thymic stromal lymphopoietin instructing dendritic cells. J Immunol, 2014-01-22;192(4):1502-11. 2014-01-22 [PMID: 24453244] (IHC, Human)
• Zhao E, Wang L, Dai J, Kryczek I, Wei S, Vatan L, Altuwaijri S, Sparwasser T, Wang G, Keller E, Zou W Regulatory T cells in the bone marrow microenvironment in patients with prostate cancer. Oncoimmunology, 2012-03-01;1(2):152-161. 2012-03-01 [PMID: 22720236] (Flow Cytometry, Human)
• Allen CE, Li L, Peters TL, Leung HC, Yu A, Man TK, Gurusiddappa S, Phillips MT, Hicks MJ, Gaikwad A, Merad M, McClain KL Cell-specific gene expression in Langerhans cell histiocytosis lesions reveals a distinct profile compared with epidermal Langerhans cells. J. Immunol., 2010-03-10;184(8):4557-67. 2010-03-10 [PMID: 20220088] (IHC-Fr, Human)
• Reinhardt RL, Hong S, Kang SJ, Wang ZE, Locksley RM Visualization of IL-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote Th1 differentiation. J. Immunol., 2006-08-01;177(3):1618-27. 2006-08-01 [PMID: 16849470] (IHC, Mouse)
• Zhang M, Wang Q, Liu Y, Sun Y, Ding G, Fu Z, Min Z, Zhu Y, Cao X Effective induction of immune tolerance by portal venous infusion with IL-10 gene-modified immature dendritic cells leading to prolongation of allograft survival. J. Mol. Med., 2004-04-01;82(4):240-9. 2004-04-01 [PMID: 15168681] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: ITGAX