CD117/c-kit Antibody (YB5.B8) [Biotin]

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Flow Cytometry: CD117/c-kit Antibody (YB5.B8) [Biotin] [NBP1-43589] - Staining of TF-1 cell line with 0.5 ug of Mouse IgG1 kappa Isotype Control Biotin (open histogram) or 0.5 ug of Anti-Human CD117 (c-Kit) Biotin ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
YB5.B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
0.5 mg/ml

CD117/c-kit Antibody (YB5.B8) [Biotin] Summary

Immunogen
Human CD117
Marker
Hematopoietic Stem Cell Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KIT
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The YB5.B8 antibody has been tested by flow cytometric analysis of the TF-1 cell line, a human erythroleukemia cell line. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Readout System

Reactivity Notes

This antibody is reactive to Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD117/c-kit Antibody (YB5.B8) [Biotin]

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

The YB5.B8 monoclonal antibody reacts with human CD117, also known as c-Kit, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells. When interested in staining HSCs from peripheral blood, we recommend the use of clone 104D2. Please refer to cat..

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CD117/c-kit Antibody (NBP1-43589)

Discover related pathways, diseases and genes to CD117/c-kit Antibody (NBP1-43589). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on CD117/c-kit.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

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Bioinformatics

Gene Symbol KIT
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