CD117/c-kit Antibody (rC117/9253) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (rC117/9253) - Azide and BSA Free [NBP3-24132] - Formalin-fixed, paraffin-embedded human GIST stained with CD117/c-kit Antibody (rC117/9253). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: rC117/9253
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD117/c-kit Antibody (rC117/9253) - Azide and BSA Free Summary

• Description: Antibody without azide - store at -20 to -80C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant full-length human CD117/c-kit protein
• Localization: Cell surface. Cytoplasm.
• Marker: Marker for Gastrointestinal Stromal Tumors
• Specificity: This monoclonal antibody recognizes a protein of 145kDa, identified as CD117/p145kit. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIT
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 2-4ug/ml
  • Immunohistochemistry-Paraffin 1-2ug/ml
• Application Notes: ELISA: For coating, order antibody without BSA.
  
  Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD117/c-kit Antibody (rC117/9253) - Azide and BSA Free

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIT