CD117/c-kit Antibody (ACK2)

Images

 
Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of Allophycocyanin conjugate of NBP1-43359. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b ...read more
Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of PE/Cy5 conjugate of NBP1-43359. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b Isotype ...read more

Product Details

Summary
Reactivity Hu, Mu, GPSpecies Glossary
Applications WB, Flow, Func, IHC, IHC-Fr, IP, IHC-WhMt
Clone
ACK2
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

CD117/c-kit Antibody (ACK2) Summary

Immunogen
Mouse CD117
Marker
Hematopoietic Stem Cell Marker
Specificity
The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
KIT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry < 0.125 ug/10^6 cells
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Immunohistochemistry Whole-Mount
Application Notes
The ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID 26680736).
Reviewed Applications
Read 1 Review rated 3
using
NBP1-43359 in the following applications:

Publications
Read Publications using
NBP1-43359 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23967247). Guinea Pig reactivity reported in scientific literature (PMID: 21654812)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD117/c-kit Antibody (ACK2)

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD117/c-kit Antibody (NBP1-43359)(4)

Review for CD117/c-kit Antibody (NBP1-43359) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-43359:
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ICC
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Images Ratings Applications Species Date Details
Immunocytochemistry CD117/c-kit NBP1-43359
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reviewed by:
Anonymous
ICC Mouse 09/02/2015
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Summary

ApplicationImmunocytochemistry
Sample TestedMouse Intestine (Ileum)
SpeciesMouse

Blocking

Blocking Details0.02% Triton-X

Secondary Antibody

Secondary DescriptionBiotinylated goat anti-rabbit IgG

Details

Detection NotesNot fully clear label, will be rerunning more samples for a better picture.
Fixation DetailsAntibody added in vitro, then fixed with 4% paraformaldehyde.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD117/c-kit Antibody (NBP1-43359). (Showing 1 - 1 of 1 FAQs).

  1. Is there a blocking peptide that can be used as a control with the c-Kit antibody (ACK2) (NBP1-43359)?
    • NBP1-46164, NBP1-46249, P4713, P4834, H00003815-P01 are all c-kit proteins that could be suitable for blocking purposes.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CD117/c-kit Antibody (NBP1-43359)

Discover related pathways, diseases and genes to CD117/c-kit Antibody (NBP1-43359). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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PTMs for CD117/c-kit Antibody (NBP1-43359)

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Research Areas for CD117/c-kit Antibody (NBP1-43359)

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Blogs on CD117/c-kit.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

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Recent Reviews

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1
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Anonymous
09/02/2015
Application: ICC
Species: Mouse

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