CD117/c-kit Antibody (2B8)

Images

 
Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R ...read more
Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IP
Clone
2B8
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD117/c-kit Antibody (2B8) Summary

Immunogen
Mouse bone marrow mast cells
Marker
Hematopoietic Stem Cell Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
KIT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.6 ug/10^6 cells in 100 uL
  • Immunocytochemistry/Immunofluorescence 0.25 ug/10^6 cells in 100 uL
  • Immunohistochemistry 1:20 - 1:1000
  • Immunohistochemistry-Frozen 1:20 - 1:1000
  • Immunoprecipitation 1:20 - 1:1000
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Use in ICC/IF reported in scientific literature (PMID 28097227).
Control
CD117/c-kit Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 1
using
NB100-77477 in the following applications:

Publications
Read Publications using
NB100-77477 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23967247).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for CD117/c-kit Antibody (2B8)

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD117/c-kit Antibody (NB100-77477)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: FLOW, Flow, ICC/IF.


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FLOW
(2)
Flow
(2)
ICC/IF
(2)
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Filter By Species
Human
(5)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB100-77477 Applications Species
Camberos V, Baio J, Bailey L et al. Effects of Spaceflight and Simulated Microgravity on YAP1 Expression in Cardiovascular Progenitors: Implications for Cell-Based Repair Int J Mol Sci Jun 4 2019 [PMID: 31167392] (Flow, Human) Flow Human
Hernandez I, Baio JM, Tsay E et al. Short-term hypoxia improves early cardiac progenitor cell functionin vitro. Am J Stem Cells. Feb 1 2018 [PMID: 29531855] (ICC/IF) ICC/IF
Baio J, Martinez AF, Bailey L et al. Spaceflight Activates Protein Kinase C Alpha Signaling and Modifies the Developmental Stage of Human Neonatal Cardiovascular Progenitor Cells Stem Cells Dev. 2018 Jun 15 [PMID: 29320953] (Human)

Details:
Citation using the DyLight 650 form of this antibody.
Human
Baio Jonathan M, Walden Ryan C, Fuentes Tania I et al. A Hyper-Crosslinked CarbohydRate Polymer Scaffold Facilitates Lineage Commitment and Maintains a Reserve Pool of ProlifeRating Cardiovascular Progenitors. Transplantation Direct 2017 [PMID: 28573188] (Flow, Human)

Details:
This citation used the DyLight 650 version of this antibody.
Flow Human
Uchida H, Machida M, Miura T et al. A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells JCI Insight Jan 12 2017 [PMID: 28097227] (ICC/IF, Human) ICC/IF Human
Meyer S, Maufort JP, Nie J et al. Development of an Efficient Targeted Cell-SELEX Procedure for DNA Aptamer Reagents. PLoS One. 2013 Aug 13 [PMID: 23967247] (FLOW, Human) FLOW Human
Charles N et al. Nat. Med. 16:701. 2010 [PMID: 20512127] (FLOW) FLOW
Bachelet I et al. J. Immunol. 180:6064. 2008 [PMID: 18424727]
Podd BS et al. J. Immunol. 176:6532. 2006 [PMID: 16709810]
Ikuta K, Weissman IL. Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation. P. Natl. Acad. Sci. USA 89:1502. 1992 [PMID: 1371359]

Review for CD117/c-kit Antibody (NB100-77477) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-77477:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD117/c-kit NB100-77477
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reviewed by:
Fumihito Noguchi
IHC-P Human 01/02/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman skin
SpeciesHuman
LotLot# B221800

Comments

CommentsWe tested CD117/c-kit antibody (2B8) for IHC-P (human skin) and confirmed that it did not work to detect human melanocytes which are reportedly positive for ckit, indicating that this product is not applicable for FFPE section.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD117/c-kit Antibody (NB100-77477) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-77477AF350
Alexa Fluor 405 NB100-77477AF405
Alexa Fluor 488 NB100-77477AF488
Alexa Fluor 532 NB100-77477AF532
Alexa Fluor 594 NB100-77477AF594
Alexa Fluor 647 NB100-77477AF647
Alexa Fluor 700 NB100-77477AF700
Alexa Fluor 750 NB100-77477AF750
Allophycocyanin NB100-77477APC
Allophycocyanin/Cy7 NB100-77477APCCY7
Biotin NB100-77477B
DyLight 350 NB100-77477UV
DyLight 405 NB100-77477V
DyLight 488 NB100-77477G
DyLight 550 NB100-77477R
DyLight 594 NB100-77477DL594
DyLight 650 NB100-77477C
DyLight 680 NB100-77477FR
DyLight 755 NB100-77477IR
FITC NB100-77477F
HRP NB100-77477H
Janelia Fluor 549 NB100-77477JF549
Janelia Fluor 646 NB100-77477JF646
PE NB100-77477PE
PE/Atto594 NB100-77477PEATT594
PE/Cy5.5 NB100-77477PECY55
PE/Cy7 NB100-77477PECY7
PerCP NB100-77477PCP

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Bioinformatics Tool for CD117/c-kit Antibody (NB100-77477)

Discover related pathways, diseases and genes to CD117/c-kit Antibody (NB100-77477). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on CD117/c-kit.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

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Recent Reviews

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1
1

Fumihito Noguchi
01/02/2018
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol KIT