CD117/c-kit Antibody (104D2) - BSA Free

Flow Cytometry: CD117/c-kit Antibody (104D2) [NBP3-09161] - (surface staining) of human peripheral blood cells with anti-CD117 (104D2) purified.

• Reactivity: Hu, Bv, Pm
• Applications: Flow, ICC/IF, IHC, IP
• Clone: 104D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD117/c-kit Antibody (104D2) - BSA Free Summary

• Additional Information: Clone 104D2 was used by HLDA to establish CD designation.
• Immunogen: MOLM-1 megakaryocytic cells
• Specificity: The mouse monoclonal antibody 104D2 detects extracellular part of CD117 / c-Kit protooncogen.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD117/c-kit Antibody (104D2) - BSA Free

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIT