CXCR5 Antibody (51505) [Unconjugated]

Images

 
CXCR5 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 5 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
CXCR5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # ...read more
Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose‑dependent manner (orange line). The amount of cells that migrated through ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-reported, ICC/IF
Clone
51505
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

CXCR5 Antibody (51505) [Unconjugated] Summary

Additional Information
Clone 51505 was used by HLDA to establish CD designation
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR5
Met1-Phe372
Accession # P32302
Specificity
Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.
Details of Functionality
ActivityAssaywCitation not found None None None None
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CXCR5
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-reported
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 0.25-1.5 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB190 in the following applications:

Publications
Read Publications using
MAB190 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR5 Antibody (51505) [Unconjugated]

  • BLR1
  • BLR1MDR-15
  • Burkitt lymphoma receptor 1
  • Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5)
  • Burkitt lymphoma receptor 1, GTP-binding protein
  • CD185 antigen
  • CD185
  • chemokine (C-X-C motif) receptor 5
  • C-X-C chemokine receptor type 5
  • CXCR5
  • CXC-R5
  • MDR15CXCR-5
  • MGC117347
  • Monocyte-derived receptor 15

Background

CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCR5 Antibody (MAB190)(21)

We have publications tested in 3 confirmed species: Human, Rat, Rabbit.

We have publications tested in 9 applications: Biacore, Flow, ICC, IHC, IHC - Frozen, IHC Frozen, IHC Paraffin-embedded, Neut, WB.


Filter By Application
Biacore
(1)
Flow
(10)
ICC
(2)
IHC
(1)
IHC - Frozen
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(1)
Neut
(2)
WB
(2)
All Applications
Filter By Species
Human
(19)
Rat
(1)
Rabbit
(1)
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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using MAB190 Applications Species
Mitkin N, Hook C, Schwartz A, Biswas S, Kochetkov D, Muratova A, Afanasyeva M, Kravchenko J, Bhattacharyya A, Kuprash D p53-dependent expression of CXCR5 chemokine receptor in MCF-7 breast cancer cells. Sci Rep, 2015;5(0):9330. 2015 [PMID: 25786345] (WB,, Human) WB Human
Chu, Ruiyin, Reczek, David, Brondyk, William Capture-stabilize approach for membrane protein SPR assays. Sci Rep, 2014;4(0):7360. 2014 [PMID: 25484112] (Biacore,, Human) Biacore Human
Rasul E, Salamon D, Nagy N, Leveau B, Banati F, Szenthe K, Koroknai A, Minarovits J, Klein G, Klein E The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia. PLoS ONE, 2014;9(8):e106008. 2014 [PMID: 25162594] (Flow,, Human) Flow Human
Humpert M, Pinto D, Jarrossay D, Thelen M CXCR7 influences the migration of B cells during maturation. Eur J Immunol, 2014;44(3):694-705. 2014 [PMID: 24259140] (Flow,, Human) Flow Human
Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency. J Allergy Clin Immunol, 2013;131(4):1146-56, 1156. 2013 [PMID: 23403044] (Flow,, Human) Flow Human
Sarra M, Cupi M, Monteleone I, Franze E, Ronchetti G, Di Sabatino A, Gentileschi P, Franceschilli L, Sileri P, Sica G, Del Vecchio Blanco G, Cretella M, Paoluzi O, Corazza G, Pallone F, Monteleone G IL-15 positively regulates IL-21 production in celiac disease mucosa. Mucosal Immunol, 2013;6(2):244-55. 2013 [PMID: 22785229] (IHC - Frozen,, Human) IHC - Frozen Human
Islam SA, Chang DS, Colvin RA Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Nat. Immunol., 2011;12(2):167-77. 2011 [PMID: 21217759] (Flow,, Human) Flow Human
Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion. Cancer Lett., 2009;283(1):29-35. 2009 [PMID: 19375853] (Neut,, Human) Neut Human
Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Bartels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, Niendorf A, Kroger N, Wenzel S, Leuwer R, Bokemeyer C, Hegewisch-Becker S, Atanackovic D Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients. Br. J. Cancer, 2008;99(6):930-8. 2008 [PMID: 18781150] (WB,, Human) WB Human
Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection. Blood, 2008;112(12):4401-10. 2008 [PMID: 18780835] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Show All 21 Publications.

Review for CXCR5 Antibody (MAB190) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB190:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 02/12/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22330139
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22330139

Details

Detection NotesSee PMID 22330139

Comments

CommentsIndependent review from antibodyresource.comThis antibody was also used for blocking experiment.

Product General Protocols

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FAQs for CXCR5 Antibody (MAB190) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled FAB190B

Additional Array Products

Bioinformatics Tool for CXCR5 Antibody (MAB190)

Discover related pathways, diseases and genes to CXCR5 Antibody (MAB190). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CXCR5 Antibody (MAB190)

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Pathways for CXCR5 Antibody (MAB190)

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Recent Reviews

5
5
1
4
0
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0
2
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1
0

 
Anonymous
02/12/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CXCR5
Entrez
Uniprot