Human CCL20/MIP-3 alpha Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human CCL20/MIP-3 alpha Quantikine ELISA Kit Summary

• Background: The Quantikine Human MIP-3 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure MIP-3 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-3 alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-3 alpha showed linear curves that were parallel to the s...tandard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIP-3 alpha.
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• Specificity: Natural and recombinant human MIP-3 alpha
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CCL20

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL20/MIP-3 alpha Quantikine ELISA Kit

• beta chemokine exodus-1
• Beta-chemokine exodus-1
• CC chemokine LARC
• C-C motif chemokine 20
• CCL20
• chemokine (C-C motif) ligand 20
• CKb4
• exodus-1
• LARC
• LARCLiver and activation-regulated chemokine
• MIP3 alpha
• MIP-3 alpha
• MIP-3a
• MIP-3-alpha
• MIP3AMacrophage inflammatory protein 3 alpha
• SCYA20Small-inducible cytokine A20
• small inducible cytokine subfamily A (Cys-Cys), member 20
• ST38

Background

CCL20, also known as MIP-3 alpha, is a chemokine that plays an important role in dendritic cell trafficking and recruitment and activation of T cells. It is upregulated in monocytes, T cells, endothelial cells, epithelial cells, and fibroblasts following inflammatory stimulation. CCL20 signals through CCR6 which is expressed on memory T cells, B cells and bone marrow-derived dendritic cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CCL20/MIP-3 alpha (DM3A00)(51)

Publications using DM3A00

• F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells bioRxiv : the preprint server for biology, 2023-02-13;0(0):. 2023-02-13 [PMID: 36789428] (Human)
• LTH Nguyen, SH Ahn, HM Shin, IJ Yang Anti-Psoriatic Effect of Rheum palmatum L. and Its Underlying Molecular Mechanisms International Journal of Molecular Sciences, 2022-12-15;23(24):. 2022-12-15 [PMID: 36555642] (Human)
• CM Yang, CC Yang, WH Hsu, LD Hsiao, HC Tseng, YF Shih Tumor Necrosis Factor-alpha-Induced C-C Motif Chemokine Ligand 20 Expression through TNF Receptor 1-Dependent Activation of EGFR/p38 MAPK and JNK1/2/FoxO1 or the NF-kappaB Pathway in Human Cardiac Fibroblasts International Journal of Molecular Sciences, 2022-08-13;23(16):. 2022-08-13 [PMID: 36012347] (Human)
• WS Wang, YK Lin, F Zhang, WJ Lei, F Pan, YN Zhu, JW Lu, CY Zhang, Q Zhou, H Ying, K Sun Single cell transcriptomic analysis of human amnion identifies cell-specific signatures associated with membrane rupture and parturition Cell & bioscience, 2022-05-18;12(1):64. 2022-05-18 [PMID: 35585644] (Human)
• M Srikanth, M Rasool 3, 3'- diindolylmethane hinders IL-17A/IL-17RA interaction and mitigates imiquimod-induced psoriasiform in mice International immunopharmacology, 2022-04-26;109(0):108795. 2022-04-26 [PMID: 35487087] (Human)
• A Jorapur, LA Marshall, S Jacobson, M Xu, S Marubayash, M Zibinsky, DX Hu, O Robles, JJ Jackson, V Baloche, P Busson, D Wustrow, DG Brockstedt, O Talay, PD Kassner, G Cutler EBV+ tumors exploit tumor cell-intrinsic and -extrinsic mechanisms to produce regulatory T cell-recruiting chemokines CCL17 and CCL22 PloS Pathogens, 2022-01-13;18(1):e1010200. 2022-01-13 [PMID: 35025968] (Human)
• N Sheshadri, DK Poria, S Sharan, Y Hu, C Yan, VN Koparde, K Balamuruga, E Sterneck PERK signaling through C/EBPdelta contributes to ER stress-induced expression of immunomodulatory and tumor promoting chemokines by cancer cells Cell Death & Disease, 2021-11-01;12(11):1038. 2021-11-01 [PMID: 34725321] (Human)
• S Ravichandr, G Grubbs, J Tang, Y Lee, C Huang, H Golding, S Khurana Systemic and mucosal immune profiling in asymptomatic and symptomatic SARS-CoV-2-infected individuals reveal unlinked immune signatures Science Advances, 2021-10-13;7(42):eabi6533. 2021-10-13 [PMID: 34644111] (Human)
• Y Wang, Z Lyu, Y Qin, X Wang, L Sun, Y Zhang, L Gong, S Wu, S Han, Y Tang, Y Jia, DL Kwong, N Kam, XY Guan FOXO1 promotes tumor progression by increased M2 macrophage infiltration in esophageal squamous cell carcinoma Theranostics, 2020-09-16;10(25):11535-11548. 2020-09-16 [PMID: 33052231] (Human)
• O Ibidapo-Ob, S Stengel, N Köse-Vogel, S Quickert, PA Reuken, M Busch, M Bauer, A Stallmach, T Bruns Mucosal-associated invariant T cells redistribute to the peritoneal cavity during spontaneous bacterial peritonitis and contribute to peritoneal inflammation Cell Mol Gastroenterol Hepatol, 2020-01-15;0(0):. 2020-01-15 [PMID: 31954178] (Human)
• K Furue, T Ito, Y Tanaka, A Hashimoto-, M Takemura, M Murata, M Kido-Nakah, G Tsuji, T Nakahara, M Furue The EGFR-ERK/JNK-CCL20 Pathway in Scratched Keratinocytes May Underpin Koebnerization in Psoriasis Patients Int J Mol Sci, 2020-01-09;21(2):. 2020-01-09 [PMID: 31936670] (Human)
• SJ Park, H Kim, SH Kim, EH Joe, I Jou Epigenetic downregulation of STAT6 increases HIF-1α expression via mTOR/S6K/S6, leading to enhanced hypoxic viability of glioma cells Acta Neuropathol Commun, 2019-09-17;7(1):149. 2019-09-17 [PMID: 31530290] (Human)
• K Furue, T Ito, Y Tanaka, A Yumine, A Hashimoto-, M Takemura, M Murata, K Yamamura, G Tsuji, M Furue Cyto/chemokine profile of in vitro scratched keratinocyte model: Implications of significant upregulation of CCL20, CXCL8 and IL36G in Koebner phenomenon J. Dermatol. Sci., 2019-04-13;0(0):. 2019-04-13 [PMID: 31010609] (Human)
• PM Schlievert, MP Cahill, BS Hostager, AJ Brosnahan, AJ Klingelhut, FA Gourronc, GA Bishop, DYM Leung Staphylococcal Superantigens Stimulate Epithelial Cells through CD40 To Produce Chemokines MBio, 2019-03-19;10(2):. 2019-03-19 [PMID: 30890614] (Human)
• P Chen, Z Zhong, H Jiang, H Chen, J Lyu, L Zhou Th17-associated cytokines multiplex testing indicates the potential of macrophage inflammatory protein-3 alpha in the diagnosis of biliary atresia Cytokine, 2019-01-23;116(0):21-26. 2019-01-23 [PMID: 30684914] (Human)
• L Wang, D Zhou, H Ren, Y Chen Effects of modified FOLFOX-6 chemotherapy on cellular immune function in patients with gastric cancer Oncol Lett, 2018-03-28;15(6):8635-8640. 2018-03-28 [PMID: 29805598] (Human)
• G Lu, X Zhang, L Shen, Q Qiao, Y Li, J Sun, J Zhang CCL20 secreted from IgA1-stimulated human mesangial cells recruits inflammatory Th17 cells in IgA nephropathy PLoS ONE, 2017-05-26;12(5):e0178352. 2017-05-26 [PMID: 28552941] (Human)
• X Ye, R Wang, R Bhattachar, D Boulbes, F Fan, L Xia, H Adoni, NJ Ajami, MC Wong, DP Smith, JF Petrosino, S Venable, W Qiao, V Baladanday, DM Maru, LM Ellis Fusobacterium nucleatum subspecies animalis influences pro-inflammatory cytokine expression and monocyte activation in human colorectal tumors Cancer Prev Res (Phila), 2017-05-08;0(0):. 2017-05-08 [PMID: 28483840] (Human)
• Valentina Cecchinato Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization J. Immunol, 2016-11-28;0(0):. 2016-11-28 [PMID: 27895171] (Human)
• CCL20 mediates RANK/RANKL-induced epithelial-mesenchymal transition in endometrial cancer cells Oncotarget, 2016-05-03;7(18):25328-39. 2016-05-03 [PMID: 27015366] (Human)
• Li W, Liu H CCL20-CCR6 Cytokine Network Facilitate Treg Activity in Advanced Grades and Metastatic Variants of Hepatocellular Carcinoma. Scand J Immunol, 2016-01-01;83(1):33-7. 2016-01-01 [PMID: 26331965] (Human)
• Clark J, Kim K, Basom R, Gharib S Plasticity of airway epithelial cell transcriptome in response to flagellin. PLoS ONE, 2015-02-10;10(2):e0115486. 2015-02-10 [PMID: 25668187] (Human)
• Zhan Z, Xie X, Cao H, Zhou X, Zhang X, Fan H, Liu Z Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination. Autophagy, 2013-12-04;10(2):257-68. 2013-12-04 [PMID: 24321786] (Human)
• Tang , Xin-Zi, Jo , Juandy, Tan , Anthony, Sandalova , Elena, Chia , Adeline, Tan , Kai Chah, Lee , Kang Hoe, Gehring , Adam J, De Libero , Gennaro, Bertoletti , Antonio IL-7 licenses activation of human liver intrasinusoidal mucosal-associated invariant T cells. J Immunol, 2013-02-27;190(7):3142-52. 2013-02-27 [PMID: 23447689] (Human)
• Lahey T, Ghosh M, Fahey JV, Shen Z, Mukura LR, Song Y, Cu-Uvin S, Mayer KH, Wright PF, Kappes JC, Ochsenbauer C, Wira CR Selective impact of HIV disease progression on the innate immune system in the human female reproductive tract. PLoS ONE, 2012-06-04;7(6):e38100. 2012-06-04 [PMID: 22675510] (Human)
• Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma. Clin. Cancer Res., 2011-11-02;17(24):7529-38. 2011-11-02 [PMID: 22048239] (Human)
• Keller MJ, Madan RP, Torres NM A randomized trial to assess anti-HIV activity in female genital tract secretions and soluble mucosal immunity following application of 1% tenofovir gel. PLoS ONE, 2011-01-25;6(1):e16475. 2011-01-25 [PMID: 21283552] (Human)
• Ramirez JM, Brembilla NC, Sorg O, Chicheportiche R, Matthes T, Dayer JM, Saurat JH, Roosnek E, Chizzolini C Activation of the aryl hydrocarbon receptor reveals distinct requirements for IL-22 and IL-17 production by human T helper cells. Eur. J. Immunol., 2010-09-01;40(9):2450-9. 2010-09-01 [PMID: 20706985] (Human)
• Charles J, Di Domizio J, Salameire D, Bendriss-Vermare N, Aspord C, Muhammad R, Lefebvre C, Plumas J, Leccia MT, Chaperot L Characterization of circulating dendritic cells in melanoma: role of CCR6 in plasmacytoid dendritic cell recruitment to the tumor. J. Invest. Dermatol., 2010-03-11;130(6):1646-56. 2010-03-11 [PMID: 20220766] (Human)
• Xu G, Zhang L, Wang DY, Xu R, Liu Z, Han DM, Wang XD, Zuo KJ, Li HB Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells. Allergy, 2009-11-25;65(5):581-9. 2009-11-25 [PMID: 19968632] (Human)
• Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis. Mediators Inflamm., 2009-07-22;2009(0):269541. 2009-07-22 [PMID: 19639049] (Human)
• Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Cancer Res., 2009-07-28;69(15):6249-55. 2009-07-28 [PMID: 19638592] (Human)
• Harper EG, Guo C, Rizzo H, Lillis JV, Kurtz SE, Skorcheva I, Purdy D, Fitch E, Iordanov M, Blauvelt A Th17 Cytokines Stimulate CCL20 Expression in Keratinocytes In Vitro and In Vivo: Implications for Psoriasis Pathogenesis. J. Invest. Dermatol., 2009-03-19;129(0):2175. 2009-03-19 [PMID: 19295614] (Human)
• Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling. J. Exp. Med., 2009-03-09;206(3):535-48. 2009-03-09 [PMID: 19273625] (Human)
• Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes. J. Immunol., 2009-03-01;182(0):3112-20. 2009-03-01 [PMID: 19234208] (Human)
• Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells. J. Exp. Med., 2009-01-12;206(1):169-81. 2009-01-12 [PMID: 19139172] (Human)
• Chang KP, Hao SP, Chang JH, Wu CC, Tsang NM, Lee YS, Hsu CL, Ueng SH, Liu SC, Liu YL, Wei PC, Liang Y, Chang YS, Yu JS Macrophage inflammatory protein-3alpha is a novel serum marker for nasopharyngeal carcinoma detection and prediction of treatment outcomes. Clin. Cancer Res., 2008-11-01;14(21):6979-87. 2008-11-01 [PMID: 18980993] (Human)
• Wang D, Drenker M, Eiz-Vesper B, Werfel T, Wittmann M Evidence for a pathogenetic role of interleukin-18 in cutaneous lupus erythematosus. Arthritis Rheum., 2008-10-01;58(10):3205-15. 2008-10-01 [PMID: 18821674] (Human)
• Peng W, Chen J, Jiang Y, Wu J, Shou Z, He Q, Wang Y, Chen Y, Wang H Urinary fractalkine is a marker of acute rejection. Kidney Int., 2008-09-17;74(11):1454-60. 2008-09-17 [PMID: 18800027] (Human)
• Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation. J. Neuroimmunol., 2008-06-09;199(1):35-45. 2008-06-09 [PMID: 18538864] (Human)
• Baumforth KR, Birgersdotter A, Reynolds GM, Wei W, Kapatai G, Flavell JR, Kalk E, Piper K, Lee S, Machado L, Hadley K, Sundblad A, Sjoberg J, Bjorkholm M, Porwit AA, Yap LF, Teo S, Grundy RG, Young LS, Ernberg I, Woodman CB, Murray PG Expression of the Epstein-Barr virus-encoded Epstein-Barr virus nuclear antigen 1 in Hodgkin's lymphoma cells mediates Up-regulation of CCL20 and the migration of regulatory T cells. Am. J. Pathol., 2008-05-23;173(1):195-204. 2008-05-23 [PMID: 18502823] (Human)
• Zrioual S, Toh ML, Tournadre A, Zhou Y, Cazalis MA, Pachot A, Miossec V, Miossec P IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood. J. Immunol., 2008-01-01;180(1):655-63. 2008-01-01 [PMID: 18097068] (Human)
• Lommatzsch M, Bratke K, Bier A, Julius P, Kuepper M, Luttmann W, Virchow JC Airway dendritic cell phenotypes in inflammatory diseases of the human lung. Eur. Respir. J., 2007-07-11;30(5):878-86. 2007-07-11 [PMID: 17626112] (Human)
• Real JM, Munro P, Buisson-Touati C, Lemichez E, Boquet P, Landraud L Specificity of immunomodulator secretion in urinary samples in response to infection by alpha-hemolysin and CNF1 bearing uropathogenic Escherichia coli. Cytokine, 2007-03-26;37(1):22-5. 2007-03-26 [PMID: 17382555] (Human)
• Demedts IK, Bracke KR, Van Pottelberge G, Testelmans D, Verleden GM, Vermassen FE, Joos GF, Brusselle GG Accumulation of dendritic cells and increased CCL20 levels in the airways of patients with chronic obstructive pulmonary disease. Am. J. Respir. Crit. Care Med., 2007-03-01;175(10):998-1005. 2007-03-01 [PMID: 17332482] (Human)
• Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. J. Exp. Med., 2006-11-20;203(12):2763-77. 2006-11-20 [PMID: 17116732] (Human)
• Fichorova RN, Trifonova RT, Gilbert RO, Costello CE, Hayes GR, Lucas JJ, Singh BN Trichomonas vaginalis lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells. Infect. Immun., 2006-10-01;74(10):5773-9. 2006-10-01 [PMID: 16988255] (Human)
• Bosco MC, Puppo M, Santangelo C, Anfosso L, Pfeffer U, Fardin P, Battaglia F, Varesio L Hypoxia modifies the transcriptome of primary human monocytes: modulation of novel immune-related genes and identification of CC-chemokine ligand 20 as a new hypoxia-inducible gene. J. Immunol., 2006-08-01;177(3):1941-55. 2006-08-01 [PMID: 16849508] (Human)
• Chavan R, Marfatia KA, An IC, Garber DA, Feinberg MB Expression of CCL20 and granulocyte-macrophage colony-stimulating factor, but not Flt3-L, from modified vaccinia virus ankara enhances antiviral cellular and humoral immune responses. J. Virol., 2006-08-01;80(15):7676-87. 2006-08-01 [PMID: 16840346] (Human)
• Peterson ML, Schlievert PM Glycerol monolaurate inhibits the effects of Gram-positive select agents on eukaryotic cells. Biochemistry, 2006-02-21;45(7):2387-97. 2006-02-21 [PMID: 16475828] (Human)
• Parameswaran K, Liang H, Fanat A, Watson R, Snider DP, O'Byrne PM Role for cysteinyl leukotrienes in allergen-induced change in circulating dendritic cell number in asthma. J. Allergy Clin. Immunol., 2004-07-01;114(1):73-9. 2004-07-01 [PMID: 15241347] (Human)

Bioinformatics

• Gene Symbol: CCL20