CCR6 Antibody (53103) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB195P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 53103
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CCR6 Antibody (53103) [Phycoerythrin] Summary

• Additional Information: Clone 53103 was used by HLDA to establish CD designation
• Immunogen: NS0 mouse myeloma cell line transfected with human CCR6
  Met1-Met374
  Accession # P51684
• Specificity: Detects human CCR6. Stains human CCR6 transfectants but not the parental cell line in flow cytometry. Does not cross-react with transfectants expressing human CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CXCR1, CXCR2, CXCR3, CXCR4, or STRL33.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR6
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR6 Antibody (53103) [Phycoerythrin]

• BN-1
• CC-CKR-6
• CCR6
• CCR-6
• CD196 antigen
• CD196
• chemokine (C-C motif) receptor 6
• CKRL3
• CKR-L3
• CKR-L3G-protein coupled receptor 29
• CKRL3LARC receptor
• CMKBR6
• CMKBR6STRL22
• DRY6
• GPR29
• GPR29C-C CKR-6
• GPRCY4
• GPR-CY4
• GPRCY4DRY-6
• lymphocyte, 22
• STRL22

Background

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR6 Antibody (FAB195P)(19)

Publications using FAB195P

• Dimova T, Brouwer M, Gosselin F, Tassignon J, Leo O, Donner C, Marchant A, Vermijlen D Effector Vgamma9Vdelta2 T cells dominate the human fetal gammadelta T-cell repertoire. Proc Natl Acad Sci U S A, 2015-01-23;112(6):E556-65. 2015-01-23 [PMID: 25617367] (Flow Cytometry, Human)
• Normanton M, Alvarenga H, Hamerschlak N, Ribeiro A, Kondo A, Rizzo L, Marti L Interleukin 7 plays a role in T lymphocyte apoptosis inhibition driven by mesenchymal stem cell without favoring proliferation and cytokines secretion. PLoS ONE, 2014-09-03;9(9):e106673. 2014-09-03 [PMID: 25184791] (Flow Cytometry, Human)
• Loubaki L, Hadj-Salem I, Fakhfakh R, Jacques E, Plante S, Boisvert M, Aoudjit F, Chakir J Co-culture of human bronchial fibroblasts and CD4+ T cells increases Th17 cytokine signature. PLoS ONE, 2013-12-05;8(12):e81983. 2013-12-05 [PMID: 24349168] (Flow Cytometry, Human)
• Cerdeira A, Rajakumar A, Royle C, Lo A, Husain Z, Thadhani R, Sukhatme V, Karumanchi S, Kopcow H Conversion of peripheral blood NK cells to a decidual NK-like phenotype by a cocktail of defined factors. J Immunol, 2013-03-13;190(8):3939-48. 2013-03-13 [PMID: 23487420] (Flow Cytometry, Human)
• Badr G, Lefevre EA, Mohany M Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis. PLoS ONE, 2011-09-01;6(9):e23741. 2011-09-01 [PMID: 21912642] (Flow Cytometry, Human)
• Dusseaux M, Martin E, Serriari N, Peguillet I, Premel V, Louis D, Milder M, Le Bourhis L, Soudais C, Treiner E, Lantz O Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells. Blood, 2010-11-17;117(4):1250-9. 2010-11-17 [PMID: 21084709] (Flow Cytometry, Human)
• Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J. Immunol., 2010-02-17;184(6):2886-98. 2010-02-17 [PMID: 20164417] (Flow Cytometry, Human)
• Campillo-Gimenez L, Cumont MC, Fay M, Kared H, Monceaux V, Diop O, Muller-Trutwin M, Hurtrel B, Levy Y, Zaunders J, Dy M, Leite-de-Moraes MC, Elbim C, Estaquier J AIDS progression is associated with the emergence of IL-17-producing cells early after simian immunodeficiency virus infection. J. Immunol., 2009-12-16;184(2):984-92. 2009-12-16 [PMID: 20018630] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• Moir S, Ho J, Malaspina A, Wang W, DiPoto AC, O'Shea MA, Roby G, Kottilil S, Arthos J, Proschan MA, Chun TW, Fauci AS Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals. J. Exp. Med., 2008-07-14;205(8):1797-805. 2008-07-14 [PMID: 18625747] (Flow Cytometry, Human)
• Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation. J. Neuroimmunol., 2008-06-09;199(1):35-45. 2008-06-09 [PMID: 18538864] (Flow Cytometry, Human)
• Pene J, Chevalier S, Preisser L, Venereau E, Guilleux MH, Ghannam S, Moles JP, Danger Y, Ravon E, Lesaux S, Yssel H, Gascan H Chronically inflamed human tissues are infiltrated by highly differentiated Th17 lymphocytes. J. Immunol., 2008-06-01;180(0):7423. 2008-06-01 [PMID: 18490742] (Flow Cytometry, Human)
• Spadaro M, Caorsi C, Ceruti P, Varadhachary A, Forni G, Pericle F, Giovarelli M Lactoferrin, a major defense protein of innate immunity, is a novel maturation factor for human dendritic cells. FASEB J., 2008-03-25;22(8):2747-57. 2008-03-25 [PMID: 18364398] (Flow Cytometry, Human)
• Nonomura C, Kikuchi J, Kiyokawa N, Ozaki H, Mitsunaga K, Ando H, Kanamori A, Kannagi R, Fujimoto J, Muroi K, Furukawa Y, Nakamura M CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1. Cancer Res., 2008-02-01;68(3):790-9. 2008-02-01 [PMID: 18245480] (Flow Cytometry, Human)
• Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients. J. Immunol., 2007-09-15;179(6):4272-82. 2007-09-15 [PMID: 17785868] (Flow Cytometry, Human)
• Facco M, Baesso I, Miorin M, Bortoli M, Cabrelle A, Boscaro E, Gurrieri C, Trentin L, Zambello R, Calabrese F, Cassatella MA, Semenzato G, Agostini C Expression and role of CCR6/CCL20 chemokine axis in pulmonary sarcoidosis. J. Leukoc. Biol., 2007-07-05;82(4):946-55. 2007-07-05 [PMID: 17615381] (Flow Cytometry, Human)
• Piovan E, Tosello V, Indraccolo S, Cabrelle A, Baesso I, Trentin L, Zamarchi R, Tamamura H, Fujii N, Semenzato G, Chieco-Bianchi L, Amadori A Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation. Blood, 2004-09-28;105(3):931-9. 2004-09-28 [PMID: 15454484] (Flow Cytometry, Human)
• Casazza S, Zappia E, Pistorio A, Gambini C Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis. Proc. Natl. Acad. Sci. U.S.A., 2004-07-19;101(30):11064-9. 2004-07-19 [PMID: 15263096] (Flow Cytometry, Human)
• Liu LY, Jarjour NN, Busse WW, Kelly EA Chemokine receptor expression on human eosinophils from peripheral blood and bronchoalveolar lavage fluid after segmental antigen challenge. J. Allergy Clin. Immunol., 2003-09-01;112(3):556-62. 2003-09-01 [PMID: 13679815] (Flow Cytometry, Human)
• Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. J. Leukoc. Biol., 2003-05-01;73(5):584-90. 2003-05-01 [PMID: 12714572] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCR6
• Uniprot:
  • P51684()