CCR6 Antibody (18B9E6) - BSA Free

Western Blot: CCR6 Antibody (18B9E6) [NBP2-25220] - A) mouse EL4, B) conA treated EL4 lysate and C) partial recombinant protein using CD196 antibody at 2 ug/ml.

Immunohistochemistry-Paraffin: CCR6 Antibody (18B9E6) - BSA Free [NBP2-25220] - CCR6 staining using GE Cell DIVE (20x) on Human Rheumatoid Arthritis synovium (10 ug/ml w/ 1:500 Donkey anti-Rat AF555). Image from verified customer review.

Immunohistochemistry-Paraffin: CCR6 Antibody (18B9E6) [NBP2-25220] - Normal mouse testis tissue stained with CD196 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining.

Product Discontinued

CCR6 Antibody (18B9E6) - BSA Free Summary

• Immunogen: A recombinant protein consisting of the external domains of mouse CD196 (CCR6) was used as the immunogen for the antibody.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: CCR6
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 2-5 ug/ml
• Application Notes: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 28684423).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CCR6 Antibody (18B9E6) - BSA Free

• BN-1
• CC-CKR-6
• CCR6
• CCR-6
• CD196 antigen
• CD196
• chemokine (C-C motif) receptor 6
• CKRL3
• CKR-L3
• CKR-L3G-protein coupled receptor 29
• CKRL3LARC receptor
• CMKBR6
• CMKBR6STRL22
• DRY6
• GPR29
• GPR29C-C CKR-6
• GPRCY4
• GPR-CY4
• GPRCY4DRY-6
• lymphocyte, 22
• STRL22

Background

CD196 (CCR6) is a chemokine receptor expressed on Th17 cells and regulatory Tcells that is induced by T-cell priming with certain cytokines. This protein plays a pivotal role in directing inflammatory and regulatory cells to inflamed tissues or the gut, where the ligand of CD196, CCL20 is expressed. Although most chemokine-chemokine receptor interactions are characterized by a high promiscuity, CD196 binds only CCL20 and -defensins. This chemokine receptor is widely expressed on human blood and tissue leukocytes, including subsets of dendritic cells, CD45RO+ effector/memory Tcells, CD25 high regulatory Tcells (Tregs), naive and memory B cells, NKT cells, and NK cells. On memory Tcells, CD196 is expressed on a population of autoreactive interleukin-10 (IL-10)-producing cells and subsets of skin- and mucosa-homing cells. More recently, CD196 was identified as a new risk factor for rheumatoid arthritis; moreover, a nucleotide polymorphism within the Cd196locus was shown to be associated with susceptibility to rheumatoid arthritis, Graves disease, and Crohn disease (Steinfelder S et al, .2011).This protein also acts as a marker for Th17 lineage polarization and HIV permissiveness in memory CD4+ Tcells. CD196+ Tcells have the potential to migrate into the GALT via the gut-homing integrin 47, a newly identified HIV-gp120 binding receptor. Nevertheless, given the ability of integrin 7 to regulate cell migration into the GALT and bind HIV-gp120, CD196+ Tcells coexpressing integrin 7 and CCR5 might have an extraordinary ability to disseminate HIV from the portal sites of entry (Monteiro P et al ,. 2011). Anti-CD196 antibody could almost completely suppress the cell migration induced by hBD-2 (Human beta-defensin-2), suggesting that hBD-2 mainly utilizes CD196 (CCR6) as a functional receptor (Niyonsaba F et al,.2004).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR6 Antibody (NBP2-25220)(6)

Publications using NBP2-25220

• Abe N, Kono M, Kono M et al. Glycogen synthase kinase 3 beta/CCR6-positive bone marrow cells correlate with disease activity in multicentric Castleman disease-TAFRO British journal of haematology 2021-12-06 [PMID: 34873687] (IHC-P, Human)
• Singh M, Singh V, Schurman JV et al. The relationship between mucosal inflammatory cells, specific symptoms, and psychological functioning in youth with irritable bowel syndrome Sci Rep 2020-07-20 [PMID: 32686762] (IF/IHC)
• Jiang X, Huang Z, Sun X et al. CCL18-NIR1/GPR3 promotes oral cancer cell growth and metastasis by activating the JAK2/STAT3 signalling pathway BMC Cancer 2020-07-10 [PMID: 32641093] (ICC/IF, IHC-P, Human)
• Singh M, Singh V, Schurman JV, Friesen CA Mucosal Th17 Cells Are Increased in Pediatric Functional Dyspepsia Associated with Chronic Gastritis Dig. Dis. Sci. 2020-01-08 [PMID: 31916087] (IF/IHC)
• Chen J, Martindale JL, Cramer C et al. The RNA-binding protein HuR contributes to neuroinflammation by promoting C-C chemokine receptor 6 (CCR6) expression on Th17 cells J. Biol. Chem. 2017-07-06 [PMID: 28684423] (WB, Human)
• Cecchinato V, Bernasconi E, Speck RF et al. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization. J. Immunol. 2017-01-01 [PMID: 27895171] (IF/IHC, Mouse)

Review for CCR6 Antibody (NBP2-25220) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 08/16/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human synovium,human tonsil
• Species: Human
• Lot: 053045589E6-04

Comments

• Comments: Heat mediated antigen retrieval - Buffer/Enzyme Used: Citrate pH 6 & TRIS pH 9     
  Permeabilisation - 0.3% Triton X-100     
  Blocking - Donkey Serum 10% + 3% BSA as blocking agent for 24 hours (10% @4°C)
  Antibody Incubation - 24 hours @4°C with 3% BSA in 1xPBS diluent
  Secondary Incubation - 1 hour @RT with 3% BSA in 1xPBS diluent

FAQs for CCR6 Antibody (NBP2-25220). (Showing 1 - 1 of 1 FAQs).

1. I would like to know the immunogen sequence of CCR6 Antibody (NBP2-25220).
   • Our product NBP2-25220 is made against all four extracellular domains of the mouse CCR6 protein (http://www.uniprot.org/uniprot/O54689#subcellular_location).

Bioinformatics

• Gene Symbol: CCR6
• Uniprot:
  • NP_001177262(Mouse)