CCR6 Antibody (140706) [Allophycocyanin]

Mouse splenocytes were stained with (A) Rat Anti-Mouse CCR6 APC-conjugated Monoclonal Antibody (Catalog # FAB590A) or (B) Rat IgG2A control antibody (Catalog # IC006A).and Rat Anti-Mouse B220/CD45R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1217F). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 140706
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

CCR6 Antibody (140706) [Allophycocyanin] Summary

• Immunogen: RBL-1 rat basophilic leukemia cell line transfected with mouse CCR6
  Met1-Met367
  Accession # O54689
• Specificity: Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCR6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR6 Antibody (140706) [Allophycocyanin]

• BN-1
• CC-CKR-6
• CCR6
• CCR-6
• CD196 antigen
• CD196
• chemokine (C-C motif) receptor 6
• CKRL3
• CKR-L3
• CKR-L3G-protein coupled receptor 29
• CKRL3LARC receptor
• CMKBR6
• CMKBR6STRL22
• DRY6
• GPR29
• GPR29C-C CKR-6
• GPRCY4
• GPR-CY4
• GPRCY4DRY-6
• lymphocyte, 22
• STRL22

Background

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR6 Antibody (FAB590A)(4)

Publications using FAB590A

• J Emgård, H Kammoun, B García-Cas, J Chesné, SM Parigi, JM Jacob, HW Cheng, E Evren, S Das, P Czarnewski, N Sleiers, F Melo-Gonza, E Kvedaraite, M Svensson, E Scandella, MR Hepworth, S Huber, B Ludewig, L Peduto, EJ Villablanc, H Veiga-Fern, JP Pereira, RA Flavell, T Willinger Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation Immunity, 2018-01-16;48(1):120-132.e8. 1/16/2018 [PMID: 29343433]
• R Robert, C Ang, G Sun, L Juglair, EX Lim, LJ Mason, NL Payne, CC Bernard, CR Mackay Essential role for CCR6 in certain inflammatory diseases demonstrated using specific antagonist and knockin mice JCI Insight, 2017-08-03;2(15):. 2017-08-03 [PMID: 28768901] (Flow Cytometry, Mouse)
• T Shi, Y He, W Sun, Y Wu, L Li, Z Jie, X Su Respiratory Syncytial virus infection compromises asthma tolerance by recruiting interleukin-17A-producing cells via CCR6-CCL20 signaling Mol. Immunol., 2017-06-07;88(0):45-57. 2017-06-07 [PMID: 28599122] (Flow Cytometry, Mouse)
• Mabuchi T, Singh T, Takekoshi T, Jia G, Wu X, Kao M, Weiss I, Farber J, Hwang S CCR6 is required for epidermal trafficking of gammadelta-T cells in an IL-23-induced model of psoriasiform dermatitis. J Invest Dermatol, 2012-08-16;133(1):164-71. 2012-08-16 [PMID: 22895364] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CCR6
• Uniprot:
  • O54689()