Rat IgG2a Isotype Control (54447) [Allophycocyanin]

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse GM-CSF Ra APC-conjugated Monoclonal Antibody (Catalog # FAB6130A, filled histogram) or Rat IgG APC-conjugated Isotype Control Antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Rt
• Applications: Ctrl
• Clone: 54447
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

Rat IgG2a Isotype Control (54447) [Allophycocyanin] Summary

• Immunogen: KLH
• Specificity: Selected for use as rat IgG_2A isotype control for flow cytometry applications.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Control 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IgG2a Isotype Control (54447) [Allophycocyanin]

• IgG2A

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2a Isotype Control (IC006A)(14)

Publications using IC006A

• Rashu, R;Ninkov, M;Wardell, CM;Benoit, JM;Wang, NI;Meilleur, CE;D'Agostino, MR;Zhang, A;Feng, E;Saeedian, N;Bell, GI;Vahedi, F;Hess, DA;Barr, SD;Troyer, RM;Kang, CY;Ashkar, AA;Miller, MS;Haeryfar, SMM; Targeting the MR1-MAIT cell axis improves vaccine efficacy and affords protection against viral pathogens PLoS pathogens 2023-06-29 [PMID: 37384813] (Flow Cytometry, Mouse)
• A Hudák, G Morgan, J Bacovsky, R Patai, TF Polgár, A Letoha, A Pettko-Sza, C Vizler, L Szilák, T Letoha Biodistribution and Cellular Internalization of Inactivated SARS-CoV-2 in Wild-Type Mice International Journal of Molecular Sciences, 2022-07-09;23(14):. 2022-07-09 [PMID: 35886958] (Flow Cytometry Control, Mouse)
• Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells. iScience, 2021-11-14;24(12):103433. 2021-11-14 [PMID: 34917892] (Flow Cytometry Control, Human)
• K Usami, K Niimi, A Matsuo, Y Suyama, Y Sakai, S Sato, K Fujihashi, H Kiyono, S Uchino, M Furukawa, J Islam, K Ito, T Moriya, Y Kusumoto, M Tomura, RC Hovey, J Sugawara, H Yoneyama, H Kitazawa, K Watanabe, H Aso, T Nochi The gut microbiota induces Peyer's-patch-dependent secretion of maternal IgA into milk Cell Reports, 2021-09-07;36(10):109655. 2021-09-07 [PMID: 34496253] (Flow Cytometry, Transgenic Mouse)
• Stella J. Berendam, Alexander F. Koeppel, Nicole R. Godfrey, Sherin J. Rouhani, Amber N. Woods, Anthony B. Rodriguez et al. Comparative Transcriptomic Analysis Identifies a Range of Immunologically Related Functional Elaborations of Lymph Node Associated Lymphatic and Blood Endothelial Cells Frontiers in Immunology 2019-04-16 [PMID: 31057546]
• Lin Ye, Yuqing Lou, Liming Lu, Xiaohong Fan Mesothelin‑targeted second generation CAR‑T cells inhibit growth of mesothelin‑expressing tumors in�vivo Experimental and Therapeutic Medicine 2018-11-27 [PMID: 30651858] (Flow Cytometry Control, Human)
• R Mashud, A Nomachi, A Hayakawa, K Kubouchi, S Danno, T Hirata, K Matsuo, T Nakayama, R Satoh, R Sugiura, M Abe, K Sakimura, S Wakana, H Ohsaki, S Kamoshida, H Mukai Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1 Sci Rep, 2017-08-09;7(1):7663. 2017-08-09 [PMID: 28794483] (Flow Cytometry, Mouse)
• Isao Kubota MafB enhances the phagocytic activity of RAW264.7 macrophages by promoting Fcgr3 expression Biochem. Biophys. Res. Commun., 2016-11-12;0(0):. 2016-11-12 [PMID: 27847322] (Flow Cytometry, Mouse)
• Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure. PLoS ONE, 2015-08-04;10(8):e0134568. 2015-08-04 [PMID: 26241657] (Flow Cytometry, Human)
• Hina Mir, Rajesh Singh, Goetz H. Kloecker, James W. Lillard, Shailesh Singh CXCR6 expression in non-small cell lung carcinoma supports metastatic process via modulating metalloproteinases Oncotarget 2015-04-30 [PMID: 25888629] (Flow Cytometry, Human)
• Fujita T, Chen M, Li B, Smith N, Peng W, Sun W, Toner M, Kress B, Wang L, Benraiss A, Takano T, Wang S, Nedergaard M Neuronal transgene expression in dominant-negative SNARE mice. J Neurosci, 2014-12-10;34(50):16594-604. 2014-12-10 [PMID: 25505312] (Flow Cytometry Control, Mouse)
• Mackay L, Rahimpour A, Ma J, Collins N, Stock A, Hafon M, Vega-Ramos J, Lauzurica P, Mueller S, Stefanovic T, Tscharke D, Heath W, Inouye M, Carbone F, Gebhardt T The developmental pathway for CD103(+)CD8+ tissue-resident memory T cells of skin. Nat Immunol, 2013-10-27;14(12):1294-301. 2013-10-27 [PMID: 24162776] (Flow Cytometry Control, Mouse)
• Giegold O, Ogrissek N, Richter C, Schroder M, Herrero San Juan M, Pfeilschifter J, Radeke H CXCL9 causes heterologous desensitization of CXCL12-mediated memory T lymphocyte activation. J Immunol, 2013-02-27;190(7):3696-705. 2013-02-27 [PMID: 23447686] (Flow Cytometry, Mouse)
• Mei HE, Frolich D, Giesecke C, Loddenkemper C, Reiter K, Schmidt S, Feist E, Daridon C, Tony HP, Radbruch A, Dorner T Steady-state generation of mucosal IgA+ plasmablasts is not abrogated by B-cell depletion therapy with rituximab. Blood, 2010-09-09;116(24):5181-90. 2010-09-09 [PMID: 20829370] (Flow Cytometry Control, Human)

Review for IgG2a Isotype Control (IC006A) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer 02/16/2018

Summary

• Lot: KEU1315031