CCR6 Antibody (140706) [Phycoerythrin]

Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Human/Mouse CCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB590P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Rat Anti-Human/Mouse CCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB590P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

gamma δ T cells in LNs can be divided into distinct subsets using CD62L and CD44 expression. (a) CD62L and CD44 expression in alpha beta CD8+ and gamma δ T cells from skin-draining peripheral (top) and gut-draining mesenteric (bottom) LNs of untreated WT mice. Numbers show frequencies of respective gates (n = 6–9 mice in 5 independent experiments, mean ± SD). (b) Total cell numbers of CD62LhiCD44lo, CD62LhiCD44hi and CD62LloCD44hi alpha beta CD8+ and gamma δ T cells from skin-draining peripheral (top) and gut-draining mesenteric (bottom) LNs of untreated WT mice (n = 5 mice in 2 independent experiments, mean ± SD, one-way ANOVA with Tukey’s multiple comparisons test, **P < 0.01; ***P < 0.001; ns: not significant). (c) CD27 and CCR6 expression in CD62LhiCD44lo, CD62LhiCD44hi and CD62LloCD44hi gamma δ T cells from skin-draining peripheral (top) and gut-draining mesenteric (bottom) LNs of untreated WT mice. Numbers show frequencies of respective gates (n = 5 mice in 2 independent experiments, mean ± SD). (d) Expression levels of S1pr1, Klf2, Ccr7 in CD62LhiCD44lo, CD62LhiCD44hi and CD62LloCD44hi alpha beta CD8+ and gamma δ T cells from mLN of untreated WT mice (n = 4 pools of 10–12 mice in 4 independent experiments, mean ± SD, one-way ANOVA with Tukey’s multiple comparisons test, AU: arbitrary units, *P < 0.05; **P < 0.01; ***P < 0.001; ns: not significant). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29895956), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu
• Applications: Flow
• Clone: 140706
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

CCR6 Antibody (140706) [Phycoerythrin] Summary

• Immunogen: RBL-1 rat basophilic leukemia cell line transfected with mouse CCR6
  Met1-Met367
  Accession # O54689
• Specificity: Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5. Detects human CCR6 in flow cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCR6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR6 Antibody (140706) [Phycoerythrin]

• BN-1
• CC-CKR-6
• CCR6
• CCR-6
• CD196 antigen
• CD196
• chemokine (C-C motif) receptor 6
• CKRL3
• CKR-L3
• CKR-L3G-protein coupled receptor 29
• CKRL3LARC receptor
• CMKBR6
• CMKBR6STRL22
• DRY6
• GPR29
• GPR29C-C CKR-6
• GPRCY4
• GPR-CY4
• GPRCY4DRY-6
• lymphocyte, 22
• STRL22

Background

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR6 Antibody (FAB590P)(20)

Publications using FAB590P

• Spencer T. Chen, Thiago Y. Oliveira, Anna Gazumyan, Melissa Cipolla, Michel C. Nussenzweig B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection Immunity 3/14/2023 [PMID: 36882061]
• Spencer T. Chen, Thiago Y. Oliveira, Anna Gazumyan, Melissa Cipolla, Michel C. Nussenzweig B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection Immunity 2023-03-14 [PMID: 36882061] (Flow Cytometry, Mouse)
• K Oishi, S Horiuchi, J Frere, RE Schwartz, BR tenOever A diminished immune response underlies age-related SARS-CoV-2 pathologies Cell Reports, 2022-06-09;39(13):111002. 2022-06-09 [PMID: 35714615] (Flow Cytometry, Hamster)
• Carina A. Bäuerlein, Musga Qureischi, Zeinab Mokhtari, Paula Tabares, Christian Brede, Ana-Laura Jordán Garrote et al. A T-Cell Surface Marker Panel Predicts Murine Acute Graft-Versus-Host Disease Frontiers in Immunology 1/29/2021 [PMID: 33584657]
• M Picard, S Yonekura, K Slowicka, I Petta, C Rauber, B Routy, C Richard, V Iebba, M Tidjani Al, S Becharef, P Ly, E Pizzato, CHK Lehmann, L Amon, C Klein, P Opolon, I Gomperts B, JY Scoazec, A Hollebecqu, D Malka, F Ghiringhel, D Dudziak, G Berx, L Vereecke, G van Loo, G Kroemer, L Zitvogel, MP Roberti Ileal immune tonus is a prognosis marker of proximal colon cancer in mice and patients Cell Death and Differentiation, 2020-12-01;0(0):. 2020-12-01 [PMID: 33262469] (ICC, Human)
• T Yamamoto, Y Endo, A Onodera, K Hirahara, HK Asou, T Nakajima, T Kanno, Y Ouchi, S Uematsu, H Nishimasu, O Nureki, DJ Tumes, N Shimojo, T Nakayama DUSP10 constrains innate IL-33-mediated cytokine production in ST2hi memory-type pathogenic Th2 cells Nat Commun, 2018-10-12;9(1):4231. 2018-10-12 [PMID: 30315197] (Flow Cytometry, Mouse)
• J Emgård, H Kammoun, B García-Cas, J Chesné, SM Parigi, JM Jacob, HW Cheng, E Evren, S Das, P Czarnewski, N Sleiers, F Melo-Gonza, E Kvedaraite, M Svensson, E Scandella, MR Hepworth, S Huber, B Ludewig, L Peduto, EJ Villablanc, H Veiga-Fern, JP Pereira, RA Flavell, T Willinger Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation Immunity, 2018-01-16;48(1):120-132.e8. 1/16/2018 [PMID: 29343433]
• Boyle S, Faulkner J, McColl S, Kochetkova M The chemokine receptor CCR6 facilitates the onset of mammary neoplasia in the MMTV-PyMT mouse model via recruitment of tumor-promoting macrophages. Mol Cancer, 2015-06-06;14(0):115. 2015-06-06 [PMID: 26047945] (Flow Cytometry, Mouse)
• Badr G, Sayed A, Abdel-Maksoud M, Mohamed A, El-Amir A, Abdel-Ghaffar F, Al-Quraishy S, Mahmoud M Infection of Female BWF1 Lupus Mice with Malaria Parasite Attenuates B Cell Autoreactivity by Modulating the CXCL12/CXCR4 Axis and Its Downstream Signals PI3K/AKT, NFkappaB and ERK. PLoS ONE, 2015-04-24;10(4):e0125340. 2015-04-24 [PMID: 25909640] (Flow Cytometry, Mouse)
• Angkasekwinai P, Sringkarin N, Supasorn O, Fungkrajai M, Wang Y, Chayakulkeeree M, Ngamskulrungroj P, Angkasekwinai N, Pattanapanyasat K Cryptococcus gattii infection dampens Th1 and Th17 responses by attenuating dendritic cell function and pulmonary chemokine expression in the immunocompetent hosts. Infect Immun, 2014-06-30;82(9):3880-90. 2014-06-30 [PMID: 24980974] (Flow Cytometry, Mouse)
• Cai Y, Xue F, Fleming C, Yang J, Ding C, Ma Y, Liu M, Zhang H, Zheng J, Xiong N, Yan J Differential developmental requirement and peripheral regulation for dermal Vgamma4 and Vgamma6T17 cells in health and inflammation. Nat Commun, 2014-06-09;5(0):3986. 2014-06-09 [PMID: 24909159] (Flow Cytometry, Mouse)
• Klein M, Brouwer M, Angele B, Geldhoff M, Marquez G, Varona R, Hacker G, Schmetzer H, Hacker H, Hammerschmidt S, van der Ende A, Pfister H, van de Beek D, Koedel U Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis. PLoS ONE, 2014-04-03;9(4):e93057. 2014-04-03 [PMID: 24699535] (Flow Cytometry, Mouse)
• Peng , Hui, Jiang , Xiaojun, Chen , Yonglin, Sojka , Dorothy, Wei , Haiming, Gao , Xiang, Sun , Rui, Yokoyama , Wayne M, Tian , Zhigang Liver-resident NK cells confer adaptive immunity in skin-contact inflammation. J Clin Invest, 2013-03-25;123(4):1444-56. 2013-03-25 [PMID: 23524967] (Flow Cytometry, Mouse)
• Cherrier M, Sawa S, Eberl G Notch, Id2, and ROR gamma-t sequentially orchestrate the fetal development of lymphoid tissue inducer cells. J. Exp. Med., 2012-03-19;209(4):729-40. 2012-03-19 [PMID: 22430492] (Flow Cytometry, Mouse)
• Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce KJ, Bu DX, Luscinskas FW, Lichtman AH Difference in Th1 and Th17 lymphocyte adhesion to endothelium. J. Immunol., 2012-01-04;188(3):1421-30. 2012-01-04 [PMID: 22219321] (Flow Cytometry, Mouse)
• Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination. J. Immunol., 2009-06-01;182(11):6851-60. 2009-06-01 [PMID: 19454681] (Flow Cytometry, Mouse)
• Atarashi K, Nishimura J, Shima T, Umesaki Y, Yamamoto M, Onoue M, Yagita H, Ishii N, Evans R, Honda K, Takeda K ATP drives lamina propria T(H)17 cell differentiation. Nature, 2008-08-20;455(7214):808-12. 2008-08-20 [PMID: 18716618] (Flow Cytometry, Mouse)
• Cox CA, Shi G, Yin H, Vistica BP, Wawrousek EF, Chan CC, Gery I Both Th1 and Th17 are immunopathogenic but differ in other key biological activities. J. Immunol., 2008-06-01;180(11):7414-22. 2008-06-01 [PMID: 18490741] (Flow Cytometry, Mouse)
• Calderon B, Suri A, Miller MJ Dendritic cells in islets of Langerhans constitutively present beta cell-derived peptides bound to their class II MHC molecules. Proc. Natl. Acad. Sci. U.S.A., 2008-04-21;105(16):6121-6. 2008-04-21 [PMID: 18427107] (Flow Cytometry, Mouse)
• Robays LJ, Maes T, Lebecque S, Lira SA, Kuziel WA, Brusselle GG, Joos GF, Vermaelen KV Chemokine receptor CCR2 but not CCR5 or CCR6 mediates the increase in pulmonary dendritic cells during allergic airway inflammation. J. Immunol., 2007-04-15;178(8):5305-11. 2007-04-15 [PMID: 17404315] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CCR6
• Uniprot:
  • O54689()