CCR3 Antibody - BSA Free

Western Blot: CCR3 Antibody [NB100-56335] - Analysis using the Azide Free version of NB100-56335. Detection of 10 ug of total cell lysate from Daudi cells with this antibody at 2 ug/ml dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CCR3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CCR3 Antibody - BSA Free (NB100-56335) is a polyclonal antibody validated for use in WB. Anti-CCR3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was raised against a synthetic peptide of human CCR3 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCR3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3 ug/ml
• Application Notes: In Daudi, a 45 kDa band should be observed.
• Control:

  CCR3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for CCR3 Antibody - BSA Free

• b-chemokine receptor
• CC chemokine receptor 3
• C-C chemokine receptor type 3
• C-C CKR-3
• CCR3
• CCR-3
• CD193 antigen
• CD193
• chemokine (C-C motif) receptor 3
• CKR3CC-CKR-3CMKBR3CD193
• eosinophil CC chemokine receptor 3
• Eosinophil eotaxin receptor
• MGC102841

Background

Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors (1). The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines. One of the CCR receptors, CCR3 (eotaxin receptor), is expressed on eosinophils and certain T cell population respond to a variety of CC chemokines apart from eotaxin, including RANTES, monocyte chemotactic protein (MCP)-2, MCP-3, and MCP-4 (2,3). CCR3 facilitated infection by a more restricted subset of primary viruses, and binding of the CCR3 ligand, eotaxin, and inhibited infection by these isolates (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR3 Antibody (NB100-56335)(2)

Publications using NB100-56335

• Messex J, Adams K, Hawkins W et al. Oncogenic Kras-Mediated Cytokine CCL15 Regulates Pancreatic Cancer Cell Migration and Invasion through ROS Cancers 2022-04-26 [PMID: 35565279]
• Abonyo BO, Alexander MS, Heiman AS. Autoregulation of CCL26 synthesis and secretion in A549 cells: a possible mechanism by which alveolar epithelial cells modulate airway inflammation. Am J Physiol Lung Cell Mol Physiol. 2005-09-01 [PMID: 15863444]

Bioinformatics

• Gene Symbol: CCR3
• Entrez:
  • 1232(Human)
  • 12771(Mouse)
  • 117027(Rat)
• Uniprot:
  • NP_847899(Human)