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Human CCL26/Eotaxin-3 DuoSet ELISA, 15 Plate

 (11 Publications)
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Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

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Human CCL26/Eotaxin-3 DuoSet ELISA, 15 Plate Summary

Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
CCL26

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DY346.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL26/Eotaxin-3 DuoSet ELISA, 15 Plate

  • CC chemokine IMAC
  • C-C motif chemokine 26
  • CCL26
  • chemokine (C-C motif) ligand 26
  • chemokine N1
  • Eotaxin3
  • Eotaxin-3
  • IMAC
  • Macrophage inflammatory protein 4-alpha
  • MGC126714
  • MIP-4a
  • MIP-4alpha
  • SCYA26
  • SCYA26MIP-4-alpha
  • small inducible cytokine A26
  • small inducible cytokine subfamily A (Cys-Cys), member 26
  • Small-inducible cytokine A26
  • TSC-1Thymic stroma chemokine-1

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IL-4 [Unconjugated]
6507-IL/CF
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CCR3 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS018) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Recombinant Human CCL11/Eotaxin (Catalog # 320‑EO) chemoattracts the BaF3 mouse proB cell line transfected with human CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant CCL11/Eotaxin (0.333 μg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155). The ND50 is typically 0.05-0.25 µg/mL.
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IL-13 [Biotin]
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TNF-alpha
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STAT6 Antibody
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Immunocytochemistry/Immunofluorescence: STAT6 Antibody (177C322.1) [NBP2-25241] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-STAT6 (177C322.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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CCL2/JE/MCP-1 [HRP]
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Albumin Antibody
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Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Cells were co-stained using Sheep Anti-Human CEBP alpha (Catalog # AF7094) and NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Albumin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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CCL17/TARC [HRP]
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MBL Antibody
AF2307
MBL Antibody
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CXCL8/IL-8
Antibody (6217) [Unco ...
MAB208
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IFN-gamma
[Unconjugated]
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MBP Antibody (12)
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Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse spinal cord ventral white matter. Image from verified customer review.
Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse brain corpus callosum. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: MBP Antibody (12) [NB600-717] - Histological and mRNA analysis of the inflammatory cytokines in the vehicle- or etifoxine-treated mice at onset of clinical symptoms. At day 10 p.i., drug treated animals showed significant differences in MBP staining. Animals treated with etifoxine showed an increase in retention of percentage of MBP coverage (*p = 0.001). Image collected and cropped by CiteAb from the following publication (http://embomolmed.embopress.org/cgi/doi/10.1002/emmm.201202124), licensed under a CC-BY licence.
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PRG2 Antibody
NBP3-04784
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Immunohistochemistry-Paraffin: PRG2 Antibody [NBP3-04784] - Human placenta using PRG2 Rabbit pAb at dilution of 1:100 (40x lens).
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Human
CCL26/Eotaxin-3 DuoSe ...
DY346
Human CCL26/Eotaxin-3 DuoSet ELISA, 15 Plate
Species: Hu
Applications: ELISA

Publications for CCL26/Eotaxin-3 (DY346)(11)

We have publications tested in 1 confirmed species: Human.


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Human
(10)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using DY346 Applications Species
N Schaunaman, KG Dimasuay, M Kraft, HW Chu Tollip interaction with STAT3: a novel mechanism to regulate human airway epithelial responses to type 2 cytokines Respiratory Research, 2022;23(1):31. 2022 [PMID: 35172835] (Human) Human
SJ Ackerman, AF Kagalwalla, I Hirano, N Gonsalves, PM Katcher, S Gupta, JB Wechsler, M Grozdanovi, Z Pan, JC Masterson, J Du, RJ Fantus, P Alumkal, JJ Lee, S Ochkur, F Ahmed, K Capocelli, H Melin-Alda, K Biette, A Dubner, K Amsden, K Keeley, M Sulkowski, A Zalewski, D Atkins, GT Furuta One-Hour Esophageal String Test: A Nonendoscopic Minimally Invasive Test That Accurately Detects Disease Activity in Eosinophilic Esophagitis Am. J. Gastroenterol., 2019;114(10):1614-1625. 2019 [PMID: 31567192] (Human) Human
JM Bratt, KY Chang, M Rabowsky, LM Franzi, SP Ott, S Filosto, T Goldkorn, M Arif, JA Last, NJ Kenyon, AA Zeki Farnesyltransferase Inhibition Exacerbates Eosinophilic Inflammation and Airway Hyperreactivity in Mice with Experimental Asthma: The Complex Roles of Ras GTPase and Farnesylpyrophosphate in Type 2 Allergic Inflammation J. Immunol., 2018;0(0):. 2018 [PMID: 29703864] (Human) Human
Y Ito, R Al Mubarak, N Roberts, K Correll, W Janssen, J Finigan, R Mishra, HW Chu IL-13 induces periostin and eotaxin expression in human primary alveolar epithelial cells: Comparison with paired airway epithelial cells PLoS ONE, 2018;13(4):e0196256. 2018 [PMID: 29672593] (Human) Human
Procyanidin A2 Modulates IL-4-Induced CCL26 Production in Human Alveolar Epithelial Cells Int J Mol Sci, 2016;17(11):. 2016 [PMID: 27845745] (Human) Human
Pavel I Novikov Eotaxin-3 as a Biomarker of Activity in Established Eosinophilic Granulomatosis with Polyangiitis J. Rheumatol., 2016;43(11):2082-2083. 2016 [PMID: 27803351] (Human) Human
Edaire Cheng JAK-STAT6 Pathway Inhibitors Block Eotaxin-3 Secretion by Epithelial Cells and Fibroblasts from Esophageal Eosinophilia Patients: Promising Agents to Improve Inflammation and Prevent Fibrosis in EoE PLoS ONE, 2016;11(6):e0157376. 2016 [PMID: 27310888] (Human) Human
Merves J, Chandramouleeswaran P, Benitez A, Muir A, Lee A, Lim D, Dods K, Mehta I, Ruchelli E, Nakagawa H, Spergel J, Wang M Altered esophageal histamine receptor expression in Eosinophilic Esophagitis (EoE): implications on disease pathogenesis. PLoS ONE, 2015;10(2):e0114831. 2015 [PMID: 25723478]
Rochman M, Kartashov A, Caldwell J, Collins M, Stucke E, Kc K, Sherrill J, Herren J, Barski A, Rothenberg M Neurotrophic tyrosine kinase receptor 1 is a direct transcriptional and epigenetic target of IL-13 involved in allergic inflammation. Mucosal Immunol, 2015;8(4):785-98. 2015 [PMID: 25389033] (Human) Human
Blanchard C, Mingler MK, Vicario M, Abonia JP, Wu YY, Lu TX, Collins MH, Putnam PE, Wells SI, Rothenberg ME IL-13 involvement in eosinophilic esophagitis: transcriptome analysis and reversibility with glucocorticoids. J. Allergy Clin. Immunol., 2007;120(6):1292-300. 2007 [PMID: 18073124] (Human) Human
Show All 11 Publications.

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Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.

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Bioinformatics

Gene Symbol CCL26