CCR2 Antibody

Images

 
Western Blot: CCR2 Antibody [NBP1-48337] - WB analysis of CCR2 in rat lysate. Image courtesy of product review submitted by Rasha Elbaz.
Immunocytochemistry/ Immunofluorescence: CCR2 Antibody [NBP1-48337] - Analysis of CCR2 in HeLa cells
Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal breast using 5 ug/ml concentration of CCR2 antibody. The breast ductal/acinar ...read more
Flow Cytometry: CCR2 Antibody [NBP1-48337] - FACS testing of CCR2 in HeLa cells.
Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human liver cancer using 5 ug/ml concentration of CCR2 antibody. Intense ...read more
Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human rectal adenocarcinoma using 5 ug/ml concentration of CCR2 antibody. The cancerous ...read more
Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using 5 ug/ml concentration of CCR2 antibody. ...read more
Immunohistochemistry-Paraffin: CCR2 Antibody [NBP1-48337] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal kidney using 5 ug/ml concentration of CCR2 antibody. Strong cytoplasmic ...read more

Product Details

Summary
Reactivity Hu, Rt, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

CCR2 Antibody Summary

Immunogen
Synthetic peptide made to an N-terminal portion of the human CCR2 protein (within residues 20-100). [Swiss-Prot# P00338]
Localization
Multi-pass cell membrane protein with extracellular and cytoplasmic domains.
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry 1:50
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
This CCR2 antibody is useful for Flow Cytometry, Immunohistochemistry-Paraffin and Immunocytochemistry/Immunofluorescence applications. Some customers have also reported success using this antibody for WB on rat and human lysates.
Positive Control
Jurkat Lysate (NB800-PC2)
HeLa Lysate (NB800-PC1)
Control Peptide
CCR2 Peptide (NBP1-48337PEP)
Publications
Read Publication using
NBP1-48337 in the following applications:

Reactivity Notes

Human. Reactivity with Rat reported by varified customer. The immunogen sequence similarity with other species: Rhesus monkey and several other primate species (100%), Mouse (69%).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CCR2 Antibody

  • C-C chemokine receptor type 2
  • C-C CKR-2
  • CC-CKR-2CKR2B
  • CCR2
  • CCR-2
  • CCR2A
  • CCR2B
  • CD192 antigen
  • CD192
  • chemokine (C-C motif) receptor 2
  • CKR2
  • CKR2A
  • CMKBR2MGC111760
  • FLJ78302
  • MCP-1 receptor
  • MCP-1-RMGC103828
  • MGC168006
  • Monocyte chemoattractant protein 1 receptor
  • monocyte chemotactic protein 1 receptor

Background

CCR2 is a major chemokine receptor expressed on monocytes and its activation through its ligands MCP1, MCP2, MCP3 and MCP4 (systematic names CCL2, CCL8, CCL7 and CCL13, respectively) stimulates the migration of monocytes across the vascular wall into tissues wherein they facilitate chronic inflammation. CCR2 is a member of GPCR1 family and is up-regulated by CREB3. It is a multi-pass membrane protein found in cell membrane, and besides monocytes, it is also found on other cell types like NK cells, macrophages, immature dendritic cells, gamma delta T cells, and activated T cells (including Th17 cells). There are two alternatively spliced forms of CCR2: CCR2A - the major isoform expressed by mononuclear cells and vascular smooth muscle cells; and CCR2B - expressed predominantly by monocytes and activated NK cells. CCR2 has both pro-inflammatory (mediated by APC and T cells) and anti-inflammatory (mediated by regulatory T cells) activities. Besides inflammation, it has been implicated several pathologies including HIV-1/AIDS, autoimmune disorders (psoriasis, rheumatoid arthritis, and multiple sclerosis), pulmonary diseases (asthma, COPD), vascular disease and cancer (prostate cancer).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-P

Publications for CCR2 Antibody (NBP1-48337)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
All Applications
Filter By Species
Human
(1)
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Reviews for CCR2 Antibody (NBP1-48337) (0)

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Product General Protocols

View specific protocols for CCR2 Antibody (NBP1-48337): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CCR2 Antibody (NBP1-48337). (Showing 1 - 1 of 1 FAQ).

  1. Can you tell if NBP1-48337 could work on mouse tissue? Based on the comparison of the UniProt sequences it is difficult to tell for the 20-100 AA sequence. Could you do a homology check with the real used immunogen and let us know if it would be worth while testing on mouse?
    • The immunogen for this antibody shares 69% similarity with mouse, so it is not predicted to cross-react. However, the immunogen also shares 69% similarity with rat and it has been shown to cross-react with rat by customers, so it may certainly work in mouse as well although it is not predicted. If you would be interested in testing this novel species, please take a look at our Innovator's Reward program.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-48337AF405
Alexa Fluor 488 Labeled NBP1-48337AF488
Alexa Fluor 647 Labeled NBP1-48337AF647
Alexa Fluor 700 Labeled NBP1-48337AF700
Biotin Labeled NBP1-48337B
DyLight 405 Labeled NBP1-48337V
DyLight 488 Labeled NBP1-48337G
DyLight 550 Labeled NBP1-48337R
DyLight 650 Labeled NBP1-48337C
FITC Labeled NBP1-48337F
HRP Labeled NBP1-48337H
PE Labeled NBP1-48337PE

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Bioinformatics Tool for CCR2 Antibody (NBP1-48337)

Discover related pathways, diseases and genes to CCR2 Antibody (NBP1-48337). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for CCR2 Antibody (NBP1-48337)

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Blogs on CCR2.

CCR2 or CD192
CCR2 is a receptor for several monocyte chemoattractant proteins (MCP1, MCP3, MCP4) that specifically govern monocyte chemotaxis. CCR2 transduces its downstream signals through increasing intracellular calcium ion levels. For example, MCP1 regulate...  Read full blog post.

CCR2: Affecting Autoimmunity via MCP1 interactions
CCR2, also known as CD192 (cluster of differentiation 192), is a chemokine receptor and is expressed by monocytes, activated T cells, B cells and natural killer cells. This protein is encoded by CCR2 gene in humans. CCR2 gene encodes two protein isofo...  Read full blog post.

MCP1: One Chemoattractant that's Hard to Resist
Monocyte Chemoattractant Protein (MCP1) is a potent monocyte attractant, is a member of the CC chemokine subfamily. MCP1 exerts its effects through binding to G-protein-coupled receptors on the surface of leukocytes targeted for activation and migrati...  Read full blog post.

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Bioinformatics

Gene Symbol CCR2
Entrez
Uniprot