CCL19/MIP-3 beta Antibody [Unconjugated]

Western blot shows conditioned media from monocyte-derived macrophages untreated (-) or treated (+) with 1 µg/mL LPS and 40 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL19/MIP-3 beta at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Re­combinant Human CCL19/MIP‑3 beta (Catalog # 361-MI) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP‑3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361). The ND50 is typically 2-10 µg/mL.

Simple Western lane view shows conditioned media from monocyte-derived macrophages untreated (-) or treated (+) with 1 μg/mL LPS and 40 ng/mL Recombinant Human IFN‑ gamma (Catalog # 285-IF) for 24 hours. A specific band was detected for CCL19/MIP‑3 beta at approximately 8 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, B/N, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCL19/MIP-3 beta Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL19/MIP-3 beta (R&D Systems, Catalog # 361-MI)
  Gly22-Ser98
  Accession # Q99731
• Specificity: Detects human CCL19/MIP-3 beta in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant mouse (rm) MIP‑3 beta , recombinant human (rh) GRO alpha , rhHCC-4, rhEotaxin, rmMIP-3 alpha , recombinant rat MIP-3 alpha , and rhMIP-3 alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCL19
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 2-10 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Human CCL19/MIP-3 beta Antibody (Catalog # AF361)
  ELISA Detection: Human CCL19/MIP-3 beta Biotinylated Antibody (Catalog # BAF361)
  Standard: Recombinant Human CCL19/MIP-3 beta (Catalog # 361-MI)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL19/MIP-3 beta Antibody [Unconjugated]

• beta chemokine exodus-3
• Beta-chemokine exodus-3
• CC chemokine ligand 19
• C-C motif chemokine 19
• CCL19
• chemokine (C-C motif) ligand 19
• CKb11
• EBI1-ligand chemokine
• ELC
• ELCMIP-3-beta
• Epstein-Barr virus-induced molecule 1 ligand chemokine
• exodus-3
• Macrophage inflammatory protein 3 beta
• macrophage inflammatory protein 3-beta
• MGC34433
• MIP3 beta
• MIP-3 beta
• MIP-3b
• MIP3BCK beta-11
• SCYA19EBI1 ligand chemokine
• small inducible cytokine subfamily A (Cys-Cys), member 19
• Small-inducible cytokine A19

Background

MIP-3 beta , also known as ELC (EBI1-Ligand Chemokine), is one of many novel beta chemokines identified through bioinformatics. MIP-3 beta cDNA encodes a 98 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is cleaved to form the 77 aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity) and the gene for MIP-3 beta  has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human beta chemokines are clustered. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. MIP-3 beta has been shown to be a unique functional ligand for CCR7 (previously referred to as the Epstein-Barr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. EBI1 is strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL19/MIP-3 beta Antibody (AF361)(9)

Publications using AF361

• Ilya Korsunsky, Kevin Wei, Mathilde Pohin, Edy Y. Kim, Francesca Barone, Triin Major et al. Cross-tissue, single-cell stromal atlas identifies shared pathological fibroblast phenotypes in four chronic inflammatory diseases Med (N Y) 2022-07-01 [PMID: 35649411]
• Astrid S Jørgensen, Pontian E Adogamhe, Julia M Laufer, Daniel F Legler, Christopher T Veldkamp, Mette M Rosenkilde et al. CCL19 with CCL21-tail displays enhanced glycosaminoglycan binding with retained chemotactic potency in dendritic cells Journal of Leukocyte Biology 2018-07-25 [PMID: 29768676]
• A Micera, A Di Zazzo, G Esposito, R Longo, W Foulsham, R Sacco, R Sgrulletta, S Bonini Age-Related Changes to Human Tear Composition Invest. Ophthalmol. Vis. Sci., 2018-04-01;59(5):2024-2031. 2018-04-01 [PMID: 29677365]
• Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus Sci Rep, 2016-07-25;6(0):29909. 2016-07-25 [PMID: 27453287] (Human)
• Markus Bruckner, Denise Dickel, Eva Singer, Daniel F. Legler Distinct modulation of chemokine expression patterns in human monocyte-derived dendritic cells by prostaglandin E(2) Cellular Immunology 2012-03-01 [PMID: 22565056]
• Birke K, Lutjen-Drecoll E, Kerjaschki D, Birke MT Expression of podoplanin and other lymphatic markers in the human anterior eye segment. Invest. Ophthalmol. Vis. Sci., 2009-09-08;51(1):344-54. 2009-09-08 [PMID: 19737877] (ICC, Human)
• Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis. Eur. J. Immunol., 2008-11-01;38(11):3050-9. 2008-11-01 [PMID: 18924211] (Blocking, Human)
• Shields JD, Fleury ME, Yong C, Tomei AA, Randolph GJ, Swartz MA Autologous chemotaxis as a mechanism of tumor cell homing to lymphatics via interstitial flow and autocrine CCR7 signaling. Cancer Cell, 2007-06-01;11(6):526-38. 2007-06-01 [PMID: 17560334] (IHC, Human)
• Espen S. Baekkevold, Takeshi Yamanaka, Roger T. Palframan, Hege S. Carlsen, Finn P. Reinholt, Ulrich H. von Andrian et al. The Ccr7 Ligand ELC (Ccl19) Is Transcytosed in High Endothelial Venules and Mediates T Cell Recruitment J. Exp. Med 2001-05-07 [PMID: 11342595] (Immunohistochemistry, Human)

Bioinformatics

• Gene Symbol: CCL19
• Uniprot:
  • Q99731()