After August 17, 2026, Novus Biologicals products and services will no longer be available on this website; you will access all products and services on rndsystems.com. Create your R&D Systems online account today.
440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880). The ND50 is typically 0.8-4 µg/mL." class="big_lightbox" target="_blank">
440-M3) was serially diluted and captured by Rat Anti-Mouse CCL19/MIP‑3 beta Monoclonal Antibody (Catalog # MAB880) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)." class="big_lightbox" target="_blank">
285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL19/MIP-3 beta at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
361-MI) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361). The ND50 is typically 2-10 µg/mL." class="big_lightbox" target="_blank">
285-IF) for 24 hours. A specific band was detected for CCL19/MIP-3 beta at approximately 8 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox" target="_blank">
361-MI) was serially diluted and captured by Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
361-MI) was serially diluted and captured by Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
Macrophage Inflammatory Protein 3 beta is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq]
![Neutralization CCL19/MIP-3 beta Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CCL19_AF880_Block_Neutralize_8981.jpg)
![Immunohistochemistry CCL19/MIP-3 beta Antibody (54909) [Unconjugated]](http://images.novusbio.com/images/antibody/CCL19_MAB361_Immunohistochemistry_7005.jpg)
![Western Blot CCL19/MIP-3 beta Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CCL19_AF361_Western_Blot_19575.jpg)
![N/A CCL19/MIP-3 beta [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_CCL19_DY361_ELISA_1963.jpg)
![N/A CCL19/MIP-3 beta [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_CCL19_DY440_ELISA_2028.jpg)
![N/A CCL19/MIP-3 beta [HRP]](http://images.novusbio.com/fullsize/qk0361_human-mip-3-beta-quantikine-quickit-elisa-9920248229..png)
![Immunocytochemistry CCL19/MIP-3 beta Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CCL19_AF880_Immunocytochemistry_9971.jpg)
![Immunocytochemistry CCL19/MIP-3 beta Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CCL19_AF880_Immunocytochemistry_7269.jpg)
![ELISA CCL19/MIP-3 beta Antibody [Unconjugated]](http://images.novusbio.com/images/af880_mouse-ccl19-mip-3-beta-affinity-purified-polyclonal-ab-elisa-639201850087386665.jpg)
![Immunocytochemistry CCL19/MIP-3 beta Antibody (54909) [Unconjugated]](http://images.novusbio.com/images/antibody/CCL19_MAB361_Immunocytochemistry_6611.jpg)
![Intracellular Staining by Flow Cytometry CCL19/MIP-3 beta Antibody (54909) [Unconjugated]](http://images.novusbio.com/images/antibody/CCL19_MAB361_Flow_Cytometry_19193.jpg)
![Neutralization CCL19/MIP-3 beta Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CCL19_AF361_Block_Neutralize_8856.jpg)
![Simple Western CCL19/MIP-3 beta Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CCL19_AF361_Simple_Western_19576.jpg)
![ELISA CCL19/MIP-3 beta Antibody [Unconjugated]](http://images.novusbio.com/images/af361_human-ccl19-mip-3-beta-affinity-purified-polyclonal-ab-elisa-639201778069734829.jpg)
![ELISA CCL19/MIP-3 beta Antibody [Unconjugated]](http://images.novusbio.com/images/af361_human-ccl19-mip-3-beta-affinity-purified-polyclonal-ab-elisa-639201850087191120.jpg)
![N/A CCL19/MIP-3 beta [HRP]](http://images.novusbio.com/fullsize/qk0361_human-mip-3-beta-quantikine-quickit-elisa-99202482356.png)
