285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
HAF017). A specific band was detected for CCL19/MIP-3 beta at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_lightbox">

285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
HAF017). A specific band was detected for CCL19/MIP-3 beta at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." alt="Western blot shows conditioned media from monocyte-derived macrophages untreated (-) or treated (+) with 1 µg/mL LPS and 40 ng/mL Recombinant Human IFN-gamma (Catalog #
285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
HAF017). A specific band was detected for CCL19/MIP-3 beta at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_thumb" />
361-MI) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog #
AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361). The ND
50 is typically 2-10 µg/mL." class="big_lightbox">

361-MI) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog #
AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361). The ND
50 is typically 2-10 µg/mL." class="big_thumb" />
285-IF) for 24 hours. A specific band was detected for CCL19/MIP-3 beta at approximately 8 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

285-IF) for 24 hours. A specific band was detected for CCL19/MIP-3 beta at approximately 8 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
361-MI) was serially diluted and captured by Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF361) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">

361-MI) was serially diluted and captured by Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF361) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />
361-MI) was serially diluted and captured by Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF361) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">

361-MI) was serially diluted and captured by Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF361) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />