CCL11/Eotaxin Antibody

Images

 
Western Blot: CCL11/Eotaxin Antibody [NBP1-77046] - HeLa cell lysate containing 10, 2.5, or 1 ng of full length recombinant eotaxin with eotaxin antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: CCL11/Eotaxin Antibody [NBP1-77046] - Immunofluorescence of Eotaxin in 293 cells with Eotaxin antibody at 10 ug/mL.
Immunocytochemistry/ Immunofluorescence: CCL11/Eotaxin Antibody [NBP1-77046] - 293 cells with Eotaxin antibody at 5 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CCL11/Eotaxin Antibody Summary

Immunogen
Antibody was raised in rabbits against a peptide corresponding to amino acids near the carboxy terminus of human eotaxin. The immunogen is located within the last 50 amino acids of Eotaxin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCL11
Purity
Ion exchange chromatography
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
Eotaxin antibody can be used for detection of eotaxin by Western blot at 0.5 to 1 mg/ml. The recombinant protein can be used as positive control and monomer and homodimer are detected at approximate 9 and 18 kDa.
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
CCL11/Eotaxin Peptide (NBP1-77046PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for CCL11/Eotaxin Antibody

  • C-C motif chemokine 11
  • CCL11
  • chemokine (C-C motif) ligand 11
  • Eosinophil chemotactic protein
  • Eotaxin
  • eotaxin-1
  • MGC22554
  • SCYA11Small-inducible cytokine A11
  • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Background

Chemokines play a key role in inflammation. The CC chemokine eotaxin is a potent and specific eosinophil chemoattractant that is expressed by a variety of cell types in certain inflammatory conditions. Some G-protein coupled chemokine receptors are also utilized as virus coreceptors for fusion and infection of cells. The eotaxin receptor CCR3 is required for HIV-1 entry into target cells such as microglia and eotaxin inhibits the infection of HIV-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Diseases for CCL11/Eotaxin Antibody (NBP1-77046)

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Bioinformatics

Gene Symbol CCL11
Entrez
Uniprot