CCL11/Eotaxin Products

420-ME) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL11/ Eotaxin (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_lightbox" target="_blank">

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">

420-ME) was serially diluted and captured by Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

420-ME) was serially diluted and captured by Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial and stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">

320-EO) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Goat Anti-Human CCL11/Eotaxin (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox" target="_blank">

MAB320) at 20ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial and stromal cells. " class="big_lightbox" target="_blank">

320-EO) was serially diluted and captured by Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-320-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

420-ME) chemoattracts the Y3 rat myeloid cell line transfected with human CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by MTT staining. Chemotaxis elicited by Recombinant Mouse CCL11/ Eotaxin (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB420). The ND₅₀ is typically 3-30 µg/mL." class="big_lightbox" target="_blank">

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to fibroblasts in submucosae. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">