CBX3 [p Ser83] Antibody - BSA Free

Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from NIH/3T3 cells, using CBX3 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.

Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from HeLa cells, using CBX3at 1:1000 dilution or HP1 gamma/CBX3 antibody. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 60s.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CBX3 [p Ser83] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CBX3 [p Ser83] Antibody - BSA Free (NBP3-38641) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic phosphorylated peptide around S83 of human CBX3 (NP_009207.2).
  
  Sequence:
  RKSLS
• Modification: p Ser83
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CBX3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for CBX3 [p Ser83] Antibody - BSA Free

• chromobox homolog 3 (Drosophila HP1 gamma)
• chromobox homolog 3
• chromobox protein homolog 3
• Drosophila)
• HECH
• Heterochromatin protein 1 homolog gamma
• heterochromatin protein HP1 gamma
• heterochromatin-like protein 1
• HP1 gamma homolog
• HP1 gamma
• HP1-GAMMA
• Modifier 2 protein

Background

HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CBX3