CBX3 Antibodies
We offer CBX3 Antibodies for use in common research applications: Dot Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each CBX3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CBX3 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the CBX3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CBX3 polyclonal antibodies and browse our CBX3 monoclonal antibody catalog.
Alternate Names for CBX3 Antibodies
anti-CBX3 antibody, anti-chromobox homolog 3 (Drosophila HP1 gamma) antibody, anti-chromobox homolog 3 antibody, anti-chromobox protein homolog 3 antibody, anti-Drosophila) antibody, anti-HECH antibody, anti-Heterochromatin protein 1 homolog gamma antibody, anti-heterochromatin protein HP1 gamma antibody, anti-heterochromatin-like protein 1 antibody, anti-HP1 gamma antibody, anti-HP1 gamma homolog antibody, anti-HP1-GAMMA antibody, anti-Modifier 2 protein antibody
16 Results for "cbx3" in Primary Antibodies
![Western Blot: CBX3 Antibody [NB100-2418] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NB100-2418-img0007.jpg "Western Blot: CBX3 Antibody [NB100-2418] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NB100-2418] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2418-img0009.jpg)
![Immunohistochemistry-Paraffin: CBX3 Antibody [NB100-2418] - Staining of paraffin embedded Human Placenta. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunohistochemistry-Paraffin-NB100-2418-img0005.jpg)
![Flow Cytometry: CBX3 Antibody [NB100-2418] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CBX3-Antibody-Flow-Cytometry-NB100-2418-img0010.jpg)
![Western Blot: CBX3 Antibody [NB100-2418] - Staining of A431 (A), HeLa (B), HepG2 (C), Jurkat (D) and K562 (E) nuclear cell lysate. 35 ug protein in RIPA buffer. Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NB100-2418-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NB100-2418] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2418-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP1-83228] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83228-img0008.jpg "Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP1-83228] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.")
![Immunohistochemistry-Paraffin: CBX3 Antibody [NBP1-83228] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunohistochemistry-Paraffin-NBP1-83228-img0010.jpg)
![Western Blot: CBX3 Antibody [NBP2-15736] - Wild-type (WT) and HP1 gamma knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HP1 gamma antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NBP2-15736-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CBX3 Antibody [NBP2-15736] - Wild-type (WT) and HP1 gamma knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HP1 gamma antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: CBX3 Antibody [NBP2-15736] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NBP2-15736-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP2-15736] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBX3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15736-img0003.jpg)
![Immunohistochemistry-Paraffin: CBX3 Antibody [NBP2-15736] - Mouse duodenum. HP1 gamma antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunohistochemistry-Paraffin-NBP2-15736-img0006.jpg)
![Immunohistochemistry-Paraffin: CBX3 Antibody [NBP2-15736] - Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunohistochemistry-Paraffin-NBP2-15736-img0002.jpg)
![Immunohistochemistry-Paraffin: CBX3 Antibody [NBP2-15736] - Mouse brain. HP1 gamma antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunohistochemistry-Paraffin-NBP2-15736-img0005.jpg)
![Immunoprecipitation: CBX3 Antibody [NBP2-15736] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extractB. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of HP1 gamma protein by 2 ug of HP1 gamma antibody 15% SDS-PAGE The immunoprecipitated HP1 gamma protein was detected by HP1 gamma antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunoprecipitation-NBP2-15736-img0007.jpg)
![Western Blot: CBX3 Antibody [NBP2-92962] - Western blot analysis of extracts from normal (control) and HP1 gamma/CBX3 knockout (KO) 293T cells, using HP1 gamma/CBX3 antibody (NBP2-92962) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NBP2-92962-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CBX3 Antibody [NBP2-92962] - Western blot analysis of extracts from normal (control) and HP1 gamma/CBX3 knockout (KO) 293T cells, using HP1 gamma/CBX3 antibody (NBP2-92962) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: CBX3 Antibody [NBP2-92962] - Analysis of extracts of various cell lines, using CBX3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NBP2-92962-img0005.jpg)
![Immunoprecipitation: CBX3 Antibody [NBP2-92962] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CBX3 antibody (NBP2-92962). Western blot was performed from the immunoprecipitate using CBX3 antibody (NBP2-92962) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunoprecipitation-NBP2-92962-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP2-92962] - Immunofluorescence analysis of C6 cells using HP1 gamma/CBX3 antibody (NBP2-92962) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92962-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP2-92962] - Analysis of U-2 OS cells using CBX3 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92962-img0001.jpg)
![Western Blot: CBX3 Antibody (1G12-1D9) [H00011335-M01] - CBX3 monoclonal antibody (M01), clone 1G12-1D9 Analysis of CBX3 expression in HeLa.](http://images.novusbio.com/fullsize/CBX3-Antibody-1G12-1D9-Western-Blot-H00011335-M01-img0007.jpg "Western Blot: CBX3 Antibody (1G12-1D9) [H00011335-M01] - CBX3 monoclonal antibody (M01), clone 1G12-1D9 Analysis of CBX3 expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody (1G12-1D9) [H00011335-M01] - Analysis of monoclonal antibody to CBX3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CBX3-Antibody-1G12-1D9-Immunocytochemistry-Immunofluorescence-H00011335-M01-img0005.jpg)
![Sandwich ELISA: CBX3 Antibody (1G12-1D9) [H00011335-M01] - Detection limit for recombinant GST tagged CBX3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CBX3-Antibody-1G12-1D9-Sandwich-ELISA-H00011335-M01-img0006.jpg)
![Immunohistochemistry: CBX3 Antibody [NB100-41429] - Sample: FFPE section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-CBX3 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunohistochemistry-NB100-41429-img0014.jpg "Immunohistochemistry: CBX3 Antibody [NB100-41429] - Sample: FFPE section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-CBX3 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CBX3 Antibody [NB100-41429] - Detection of Human CBX3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBX3 antibody NB100-41429 used for IP at 3 mcg/mg lysate. CBX3 was also immunoprecipitated by rabbit anti-CBX3 antibodies NB100-41427 and NB100-41428, which recognize upstream epitopes. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunoprecipitation-NB100-41429-img0001.jpg)
![Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368] - Western blot analysis of extracts of various cell lines, using CBX3 Rabbit mAb (NBP3-16368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/CBX3-Antibody-7B7Q2-Western-Blot-NBP3-16368-img0002.jpg "Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368] - Western blot analysis of extracts of various cell lines, using CBX3 Rabbit mAb (NBP3-16368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: CBX3 Antibody (7B7Q2) [NBP3-16368] - Immunohistochemistry of paraffin-embedded human colon using CBX3 Rabbit mAb (NBP3-16368) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CBX3-Antibody-7B7Q2-Immunohistochemistry-Paraffin-NBP3-16368-img0001.jpg)
![Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368] - Western blot analysis of extracts of Rat brain, using CBX3 Rabbit mAb (NBP3-16368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/CBX3-Antibody-7B7Q2-Western-Blot-NBP3-16368-img0003.jpg)
![Western Blot: CBX3 Antibody [NBP2-98758] - Anti-CBX3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NBP2-98758-img0003.jpg "Western Blot: CBX3 Antibody [NBP2-98758] - Anti-CBX3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa (We are unsure as to the identity of these extra bands).")
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP2-98758] - Immunofluorescence staining of CBX3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CBX3 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98758-img0002.jpg)
![Immunoprecipitation: CBX3 Antibody [NBP2-98758] - CBX3 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate 2 ul anti-CBX3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CBX3 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunoprecipitation-NBP2-98758-img0001.jpg)
![Western Blot: CBX3 Antibody [NBP2-98757] - Anti-CBX3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NBP2-98757-img0002.jpg "Western Blot: CBX3 Antibody [NBP2-98757] - Anti-CBX3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa")
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP2-98757] - Immunofluorescence staining of CBX3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CBX3 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to .](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98757-img0001.jpg)
![Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from NIH/3T3 cells, using CBX3 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38641_rabbit-cbx3-p-ser-83-pab-101220241243937.jpg "Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from NIH/3T3 cells, using CBX3 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.")
![Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from HeLa cells, using CBX3at 1:1000 dilution or HP1 gamma/CBX3 antibody. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38641_rabbit-cbx3-p-ser-83-pab-1012202411572645.jpg)
![Dot Blot: CBX3 Antibody [NBP3-35891] - Dot-blot analysis of all sorts of peptides using CBX3 Rabbit pAb at 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp3-35891_rabbit-cbx3-pab-912202414201334.jpg "Dot Blot: CBX3 Antibody [NBP3-35891] - Dot-blot analysis of all sorts of peptides using CBX3 Rabbit pAb at 1:1000 dilution.")