| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83228-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92962-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98758-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98757-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38641-20ul | |||
![Western Blot: CBX3 Antibody [NB100-2418] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NB100-2418-img0007.jpg "Western Blot: CBX3 Antibody [NB100-2418] - Staining of NIH3T3 nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NB100-2418] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2418-img0009.jpg)
![Immunohistochemistry-Paraffin: CBX3 Antibody [NB100-2418] - Staining of paraffin embedded Human Placenta. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunohistochemistry-Paraffin-NB100-2418-img0005.jpg)
![Flow Cytometry: CBX3 Antibody [NB100-2418] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CBX3-Antibody-Flow-Cytometry-NB100-2418-img0010.jpg)
![Western Blot: CBX3 Antibody [NB100-2418] - Staining of A431 (A), HeLa (B), HepG2 (C), Jurkat (D) and K562 (E) nuclear cell lysate. 35 ug protein in RIPA buffer. Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NB100-2418-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NB100-2418] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2418-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP1-83228] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83228-img0008.jpg "Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP1-83228] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.")
![Immunohistochemistry-Paraffin: CBX3 Antibody [NBP1-83228] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunohistochemistry-Paraffin-NBP1-83228-img0010.jpg)
![Western Blot: CBX3 Antibody [NBP2-15736] - Wild-type (WT) and HP1 gamma knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HP1 gamma antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NBP2-15736-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CBX3 Antibody [NBP2-15736] - Wild-type (WT) and HP1 gamma knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HP1 gamma antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: CBX3 Antibody [NBP2-15736] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NBP2-15736-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP2-15736] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBX3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15736-img0003.jpg)
![Immunohistochemistry-Paraffin: CBX3 Antibody [NBP2-15736] - Mouse duodenum. HP1 gamma antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunohistochemistry-Paraffin-NBP2-15736-img0006.jpg)
![Immunohistochemistry-Paraffin: CBX3 Antibody [NBP2-15736] - Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunohistochemistry-Paraffin-NBP2-15736-img0002.jpg)
![Immunohistochemistry-Paraffin: CBX3 Antibody [NBP2-15736] - Mouse brain. HP1 gamma antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunohistochemistry-Paraffin-NBP2-15736-img0005.jpg)
![Immunoprecipitation: CBX3 Antibody [NBP2-15736] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extractB. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of HP1 gamma protein by 2 ug of HP1 gamma antibody 15% SDS-PAGE The immunoprecipitated HP1 gamma protein was detected by HP1 gamma antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunoprecipitation-NBP2-15736-img0007.jpg)
![Western Blot: CBX3 Antibody [NBP2-92962] - Western blot analysis of extracts from normal (control) and HP1 gamma/CBX3 knockout (KO) 293T cells, using HP1 gamma/CBX3 antibody (NBP2-92962) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NBP2-92962-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CBX3 Antibody [NBP2-92962] - Western blot analysis of extracts from normal (control) and HP1 gamma/CBX3 knockout (KO) 293T cells, using HP1 gamma/CBX3 antibody (NBP2-92962) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: CBX3 Antibody [NBP2-92962] - Analysis of extracts of various cell lines, using CBX3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NBP2-92962-img0005.jpg)
![Immunoprecipitation: CBX3 Antibody [NBP2-92962] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CBX3 antibody (NBP2-92962). Western blot was performed from the immunoprecipitate using CBX3 antibody (NBP2-92962) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunoprecipitation-NBP2-92962-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP2-92962] - Immunofluorescence analysis of C6 cells using HP1 gamma/CBX3 antibody (NBP2-92962) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92962-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP2-92962] - Analysis of U-2 OS cells using CBX3 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92962-img0001.jpg)
![Western Blot: CBX3 Antibody (1G12-1D9) [H00011335-M01] - CBX3 monoclonal antibody (M01), clone 1G12-1D9 Analysis of CBX3 expression in HeLa.](http://images.novusbio.com/fullsize/CBX3-Antibody-1G12-1D9-Western-Blot-H00011335-M01-img0007.jpg "Western Blot: CBX3 Antibody (1G12-1D9) [H00011335-M01] - CBX3 monoclonal antibody (M01), clone 1G12-1D9 Analysis of CBX3 expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody (1G12-1D9) [H00011335-M01] - Analysis of monoclonal antibody to CBX3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CBX3-Antibody-1G12-1D9-Immunocytochemistry-Immunofluorescence-H00011335-M01-img0005.jpg)
![Sandwich ELISA: CBX3 Antibody (1G12-1D9) [H00011335-M01] - Detection limit for recombinant GST tagged CBX3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CBX3-Antibody-1G12-1D9-Sandwich-ELISA-H00011335-M01-img0006.jpg)
![Immunohistochemistry: CBX3 Antibody [NB100-41429] - Sample: FFPE section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-CBX3 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunohistochemistry-NB100-41429-img0014.jpg "Immunohistochemistry: CBX3 Antibody [NB100-41429] - Sample: FFPE section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-CBX3 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CBX3 Antibody [NB100-41429] - Detection of Human CBX3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBX3 antibody NB100-41429 used for IP at 3 mcg/mg lysate. CBX3 was also immunoprecipitated by rabbit anti-CBX3 antibodies NB100-41427 and NB100-41428, which recognize upstream epitopes. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunoprecipitation-NB100-41429-img0001.jpg)
![Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368] - Western blot analysis of extracts of various cell lines, using CBX3 Rabbit mAb (NBP3-16368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/CBX3-Antibody-7B7Q2-Western-Blot-NBP3-16368-img0002.jpg "Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368] - Western blot analysis of extracts of various cell lines, using CBX3 Rabbit mAb (NBP3-16368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: CBX3 Antibody (7B7Q2) [NBP3-16368] - Immunohistochemistry of paraffin-embedded human colon using CBX3 Rabbit mAb (NBP3-16368) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CBX3-Antibody-7B7Q2-Immunohistochemistry-Paraffin-NBP3-16368-img0001.jpg)
![Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368] - Western blot analysis of extracts of Rat brain, using CBX3 Rabbit mAb (NBP3-16368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/CBX3-Antibody-7B7Q2-Western-Blot-NBP3-16368-img0003.jpg)
![Western Blot: CBX3 Antibody [NBP2-98758] - Anti-CBX3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NBP2-98758-img0003.jpg "Western Blot: CBX3 Antibody [NBP2-98758] - Anti-CBX3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa (We are unsure as to the identity of these extra bands).")
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP2-98758] - Immunofluorescence staining of CBX3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CBX3 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98758-img0002.jpg)
![Immunoprecipitation: CBX3 Antibody [NBP2-98758] - CBX3 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate 2 ul anti-CBX3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CBX3 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunoprecipitation-NBP2-98758-img0001.jpg)
![Western Blot: CBX3 Antibody [NBP2-98757] - Anti-CBX3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa](http://images.novusbio.com/fullsize/CBX3-Antibody-Western-Blot-NBP2-98757-img0002.jpg "Western Blot: CBX3 Antibody [NBP2-98757] - Anti-CBX3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa")
![Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP2-98757] - Immunofluorescence staining of CBX3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CBX3 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to .](http://images.novusbio.com/fullsize/CBX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98757-img0001.jpg)
![Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from NIH/3T3 cells, using CBX3 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38641_rabbit-cbx3-p-ser-83-pab-101220241243937.jpg "Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from NIH/3T3 cells, using CBX3 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.")
![Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from HeLa cells, using CBX3at 1:1000 dilution or HP1 gamma/CBX3 antibody. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38641_rabbit-cbx3-p-ser-83-pab-1012202411572645.jpg)
![ELISA: Mouse CBX3 ELISA Kit (Colorimetric) [NBP2-75056] - Samples were spiked with high concentrations of Mouse CBX3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75056_mouse-cbx3-elisa-kit-colorimetric-132202416235734.jpg "ELISA: Mouse CBX3 ELISA Kit (Colorimetric) [NBP2-75056] - Samples were spiked with high concentrations of Mouse CBX3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse CBX3 ELISA Kit (Colorimetric) [NBP2-75056] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75056_mouse-cbx3-elisa-kit-colorimetric-432024917343.jpg)
![ELISA: Human CBX3 ELISA Kit (Colorimetric) [NBP2-75055] - Samples were spiked with high concentrations of Human CBX3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75055_human-cbx3-elisa-kit-colorimetric-132202416133615.jpg "ELISA: Human CBX3 ELISA Kit (Colorimetric) [NBP2-75055] - Samples were spiked with high concentrations of Human CBX3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human CBX3 ELISA Kit (Colorimetric) [NBP2-75055] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75055_human-cbx3-elisa-kit-colorimetric-4320249163395.jpg)