hnRNP A2B1 Antibody (DP3B3)

Images

 
Western Blot: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - Whole cell protein from PC12 (lane 1), NIH-3T3 (lane 2), A431 (lane 3) and HeLa (lane 4) was separated by SDS-PAGE and transferred to PVDF membrane. The membrane ...read more
Immunocytochemistry/ Immunofluorescence: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - The hnRNP A2B1 antibody was tested in HeLa cells at a 5ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and ...read more
Western Blot: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - hnRNP A2B1 expression in HeLa cells. Lane 1= 15 ug of HeLa nuclear extracts Lane 2= Co-IP of hnRNP A2B1. Image and data from confirmed customer review.
Western Blot: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - Cell line lysates were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-hnRNP-A2/B1 Clone: DP3B3. The antibody was developed using Goat Anti-Mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, PmSpecies Glossary
Applications WB, ICC/IF, IP
Clone
DP3B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

hnRNP A2B1 Antibody (DP3B3) Summary

Immunogen
Bacterially expressed human hnRNP A2 fusion protein.
Localization
Nuclear
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HNRNPA2B1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Control
NIH 3T3 Whole Cell Lysate
A-431 Whole Cell Lysate
HeLa Whole Cell Lysate
PC-12 Whole Cell Lysate
hnRNP A2B1 Knockout 293T Cell Lysate
Publications
Read Publications using
NB120-6102 in the following applications:

Reactivity Notes

Mouse, Human, Chicken, Canine, Rat, Monkey, Bovine and Hamster.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for hnRNP A2B1 Antibody (DP3B3)

  • DKFZp779B0244
  • FLJ22720
  • heterogeneous nuclear ribonucleoprotein A2/B1
  • heterogeneous nuclear ribonucleoprotein B1
  • heterogeneous nuclear ribonucleoproteins A2/B1
  • hnRNP A2 / hnRNP B1
  • HNRNPA2
  • HNRNPB1
  • HNRPA2
  • HNRPA2B1hnRNP A2/B1
  • HNRPB1
  • nuclear ribonucleoprotein particle A2 protein
  • RNPA2
  • SNRPB1

Background

RNA polymerase II transcripts in the nucleus are in complex with several different proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins act in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA ranslation, and turnover.2. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts. Isolated hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform.3 The major steadystate proteins of the isolated hnRNP complex are the A1, A2, B1, B2, C1, and C2 with a range of molecular weights (34-43 kDa).3 hnRNP-A2/B1 proteins are located in the nucleus of most tissues cells; however, the hnRNP-A2 protein is found also in the cytoplasm of skin and esophagus cells. In rat brain, hnRNP-A2/B1 proteins are found in neurons in the cerebral cortices, hippocampal formation, olfactory regions, caudate-putamin, and the supraoptic ucleus of the hypothalamus.4 Expression of hnRNP-A2/B1 proteins was found also in lung development and its over expression correlated with lung cancer.5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for hnRNP A2B1 Antibody (NB120-6102)(3)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(1)
All Applications
Filter By Species
Mouse
(1)
Rat
(1)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for hnRNP A2B1 Antibody (NB120-6102) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB120-6102AF350
Alexa Fluor 405 NB120-6102AF405
Alexa Fluor 488 NB120-6102AF488
Alexa Fluor 532 NB120-6102AF532
Alexa Fluor 594 NB120-6102AF594
Alexa Fluor 647 NB120-6102AF647
Alexa Fluor 700 NB120-6102AF700
Alexa Fluor 750 NB120-6102AF750
Biotin NB120-6102B
DyLight 350 NB120-6102UV
DyLight 405 NB120-6102V
DyLight 488 NB120-6102G
DyLight 550 NB120-6102R
DyLight 594 NB120-6102DL594
DyLight 650 NB120-6102C
DyLight 680 NB120-6102FR
DyLight 755 NB120-6102IR
HRP NB120-6102H
Janelia Fluor 549 NB120-6102JF549
Janelia Fluor 646 NB120-6102JF646

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Bioinformatics

Gene Symbol HNRNPA2B1
Entrez
Uniprot