CHAF1B Antibody (SS 53)

Images

 
Western Blot: CHAF1B Antibody (SS 53) [NB500-212] - A. RKO cells with CAF-1 p150, CAF-1 p60 and PCNA antibodies. B. p150 RNAi knockdown. Reference Pubmed 14519857.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP, KO
Clone
SS 53
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CHAF1B Antibody (SS 53) Summary

Immunogen
Recombinant human CHAF1B [UniProt# Q13112]
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHAF1B
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 1:10-1:500
  • Knockout Validated
Application Notes
This CHAF1B (SS 53) antibody is useful in Western blot and Immunoprecipitation. Immunocytochemistry/Immunofluorescence was reported in scientific literature. In WB a band can be seen at ~60 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CHAF1B Lysate (NBL1-09140)
Publications
Read Publications using
NB500-212 in the following applications:

  • 2 publications
  • IP
    2 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for CHAF1B Antibody (SS 53)

  • CAF1
  • CAF-1
  • CAF1ACAF-1 subunit B
  • CAF1P60CAF-I 60 kDa subunit
  • CAF-IP60
  • chromatin assembly factor 1 subunit B
  • chromatin assembly factor 1, subunit B (p60)
  • Chromatin assembly factor I p60 subunit
  • human chromatin assembly factor-I p60 subunit, 10
  • MPHOSPH7CAF-I p60
  • MPP7M-phase phosphoprotein 7

Background

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CHAF1B Antibody (NB500-212)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHAF1B
Entrez
Uniprot