CHAF1B Antibody (SS 53)

Knockdown Validated: CHAF1B Antibody (SS 53) [NB500-212] - A. RKO cells with CAF-1 p150, CAF-1 p60 and PCNA antibodies. B. p150 RNAi knockdown. Reference Pubmed 14519857.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP, KD
• Clone: SS 53
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CHAF1B Antibody (SS 53) Summary

• Immunogen: Recombinant human CHAF1B [UniProt# Q13112]
• Localization: Nuclear
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHAF1B
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence reported in scientific literature
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:100-1:2000
• Application Notes: In WB a band can be seen at ~60 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CHAF1B Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for CHAF1B Antibody (SS 53)

• CAF1
• CAF-1
• CAF1ACAF-1 subunit B
• CAF1P60CAF-I 60 kDa subunit
• CAF-IP60
• chromatin assembly factor 1 subunit B
• chromatin assembly factor 1, subunit B (p60)
• Chromatin assembly factor I p60 subunit
• human chromatin assembly factor-I p60 subunit, 10
• MPHOSPH7CAF-I p60
• MPP7M-phase phosphoprotein 7

Background

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHAF1B Antibody (NB500-212)(8)

Publications using NB500-212

• Gonzalez-Rico Fj, Vicente-Garcia C, Fernandez A et Al. Alu retrotransposons modulate Nanog expression through dynamic changes in regional chromatin conformation via aryl hydrocarbon receptor Epigenetics Chromatin 2020-03-14 [PMID: 32169107]
• Bakail M, Gaubert A, Andreani J et al. Design on a Rational Basis of High-Affinity Peptides Inhibiting the Histone Chaperone ASF1 Cell Chem Biol 2019-09-18 [PMID: 31543461]
• Cohen C, Corpet A, Maroui MA et al. Promyelocytic Leukemia (PML) Nuclear Bodies (NBs) Induce Latent/Quiescent HSV-1 Genomes Chromatinization Through a PML-NB/Histone H3.3/H3.3 Chaperone Axis. bioRxiv [PMID: 30235352] (ICC/IF, Human)
• Elsaesser, SJ, Allis, CD. HIRA and Daxx Constitute Two Independent Histone H3.3-Containing Predisposition Complexes. Cold Spring Harb Symp Quant Biol. 2010-11-03 [PMID: 21047901]
• Galvani, A et al. In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells. Mol Cell Biol;28(11):3672-85. 2008-06-01 [PMID: 18378699] (IP, WB, Human)
• Green, CM, Almouzni, G. Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo. EMBO J;22(19):5163-74. 2003-10-01 [PMID: 14517254] (ICC/IF, Human)
• Shibahara, K, Stillman, B. Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. Cell;96(4):575-85. 1999-02-19 [PMID: 10052459]
• Smith, S, Stillman, B. Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro. J Biol Chem;266(18):12041-7. 1991-06-25 [PMID: 2050697] (WB, IP, ICC/IF, Human)

Bioinformatics

• Gene Symbol: CHAF1B
• Entrez:
  • 8208(Human)
• Uniprot:
  • Q13112(Human)