Cathepsin E Antibody - BSA Free

Western Blot: Cathepsin E Antibody [NBP2-33868] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Immunohistochemistry-Paraffin: Cathepsin E Antibody [NBP2-33868] - Staining in human stomach and liver tissues using anti-CTSE antibody. Corresponding CTSE RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Cathepsin E Antibody [NBP2-33868] - Staining of human liver shows low expression as expected.

Immunohistochemistry-Paraffin: Cathepsin E Antibody [NBP2-33868] - Staining of human stomach shows high expression.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cathepsin E Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Cathepsin E Antibody - BSA Free (NBP2-33868) is a polyclonal antibody validated for use in IHC, WB and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: VEGLTVVGQQFGESVTEPGQTFVDAEFDGILGLGYPSLAVGGVTPVFDNMMAQNLVDLPMFSVYMSSNPEGGAGSELIFGGYDHSHFSGSLNWVPVTKQAYWQIALDN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTSE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Simple Western
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
  See Simple Western Antibody Database for Simple Western validation: separated by Size
• Control Peptide:

  Cathepsin E Protein (NBP2-33868PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Cathepsin E Antibody - BSA Free

• CATE
• Cathepsin E
• CTSE
• EC 3.4.23
• EC 3.4.23.34
• erythrocyte membrane aspartic proteinase
• slow-moving proteinase

Background

Cathepsin E (CATE) is a gastric aspartyl protease member of the peptidase C1 family that functions as a disulfide-linked homodimer. Cathepsin E may have a role in immune function. It is most likely involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. Cathepsin E may also play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.

Cathepsin E is commonly linked to gastric cancer, and Cathepsin E antibodies have emerged as important reagents for gastric cancer studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTSE
• Uniprot:
  • P14091(Human)