Carbonic Anhydrase IX/CA9 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Carbonic Anhydrase IX/CA9 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX (R&D Systems, Catalog # 2188-CA)
  Pro59-Asp414
  Accession # Q16790
• Specificity: Detects human Carbonic Anhydrase IX (CA9) in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity with recombinant mouse (rm) CA9, rhCA12, and rhCA14 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CA9
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human Carbonic Anhydrase IX/CA9 Antibody (Catalog # MAB2188)
  ELISA Detection:Human Carbonic Anhydrase IX Biotinylated Antibody (Catalog # BAF2188)
  Standard: Recombinant Human Carbonic Anhydrase IX/CA9 (Catalog # 2188-CA)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase IX/CA9 Antibody [Biotin]

• CA9
• CAIX
• CA-IX
• Carbonate dehydratase IX
• carbonic anhydrase 9
• Carbonic Anhydrase IX
• carbonic dehydratase
• EC 4.2.1.1
• G250
• Membrane antigen MN
• MN
• P54/58N
• PMW1
• RCC
• RCC-associated antigen G250
• RCC-associated protein G250
• Renal cell carcinoma-associated antigen G250

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1 - 3). Topics in the CA meeting (6^th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.

1. Pastorek, J. et al. (1994) Oncogene 9: 2877.
2. Opavsky, R. et al. (1996) Genomics 33: 480.
3. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
4. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
5. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carbonic Anhydrase IX/CA9 Antibody (BAF2188)(2)

Publications using BAF2188

• Rory M. Bade, Jennifer L. Schehr, Hamid Emamekhoo, Benjamin K. Gibbs, Tamara S. Rodems, Matthew C. Mannino et al. Development and initial clinical testing of a multiplexed circulating tumor cell assay in patients with clear cell renal cell carcinoma Molecular Oncology 9/1/2021 [PMID: 33604999]
• S Liu, Z Tian, L Zhang, S Hou, S Hu, J Wu, Y Jing, H Sun, F Yu, L Zhao, R Wang, HR Tseng, HE Zhau, LW Chung, K Wu, H Wang, JB Wu, Y Nie, C Shao Combined cell surface carbonic anhydrase 9 and CD147 antigens enable high-efficiency capture of circulating tumor cells in clear cell renal cell carcinoma patients Oncotarget, 2016-09-13;7(37):59877-59891. 2016-09-13 [PMID: 27494883] (Functional Assay, Human)

Bioinformatics

• Gene Symbol: CA9
• Uniprot:
  • Q16790()