Western Blot: Capicua Antibody [NB110-59906] - WB detection of Human Capicua /CIC in whole cell lysates of HeLa (5, 15 and 50 ug ) and 293T (T, 50 ug) cells using affinity purified rabbit Capicua antibody NB110-59906 ...read more
Immunocytochemistry/ Immunofluorescence: Capicua Antibody [NB110-59906] - Capicua antibody was tested in Neuro 2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Immunoprecipitation: Capicua Antibody [NB110-59906] - IP detection of Human Capicua / CIC in 1 mg of whole cell lysate from HeLa cells (20% of IP loaded) using affinity purified rabbit polyclonal Capicua antibody ...read more
This Capicua antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitationn and Western blot. In Western blot a band is observed at ~250 kDa. CIC (~164 kDa) interacts with Ataxin 1 (~87 kDa) which may explain the band observed at 250 kDa (complex). In ICC/IF nuclear staining was observed in Neuro2A cells.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
250 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Capicua is a transcriptional repressor protein belonging to HMG-box proteins which has been suggested to play a key role in CNS development and is expressed in foetal brain as well as medulloblastomas. Capicua protein expression levels are subject to posttranscriptional regulation by signaling downstream of two RTKs - EGFR and Torso (that propagate their signals through Ras-Raf-MAPK signaling cascade) and MAPK-mediated phosphorylation promotes capicua degradation. Capicua has been shown to act as a transcriptional repressor through interaction with a co-repressor Groucho. Moreover, Bantam which is a Hippo pathway player has recently been shown to acts in a feedback loop to limit Capicua expression for regulating EGFR signaling. Capicua is well conserved in mammals, and mediates RTK-dependent responses that are linked to cell proliferation, cell differentiation and cancer. Capicua can interact with ATXN1 as well as ATXN1L, and has been shown to be involved in Spinocerebellar Ataxia Type 1 (SCA-1).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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