Capicua Antibody

Images

 
Western Blot: Capicua Antibody [NB110-59906] - WB detection of Human Capicua /CIC in whole cell lysates of HeLa (5, 15 and 50 ug ) and 293T (T, 50 ug) cells using affinity purified rabbit Capicua antibody NB110-59906 ...read more
Immunocytochemistry/ Immunofluorescence: Capicua Antibody [NB110-59906] - Capicua antibody was tested in Neuro 2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
Immunoprecipitation: Capicua Antibody [NB110-59906] - IP detection of Human Capicua / CIC in 1 mg of whole cell lysate from HeLa cells (20% of IP loaded) using affinity purified rabbit polyclonal Capicua antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Capicua Antibody Summary

Immunogen
The epitope recognized by this antibody maps to a C-terminal region within residue 1500-1608 of human Capicua. [NP_055940.3]
Clonality
Polyclonal
Host
Rabbit
Gene
CIC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2500
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
This Capicua antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitationn and Western blot. In Western blot a band is observed at ~250 kDa. CIC (~164 kDa) interacts with Ataxin 1 (~87 kDa) which may explain the band observed at 250 kDa (complex). In ICC/IF nuclear staining was observed in Neuro2A cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)

Reactivity Notes

Human, mouse, rat, canine.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Capicua Antibody

  • capicua homolog (Drosophila)
  • KIAA0306capicua (Drosophila) homolog
  • protein capicua homolog

Background

Capicua is a transcriptional repressor protein belonging to HMG-box proteins which has been suggested to play a key role in CNS development and is expressed in foetal brain as well as medulloblastomas. Capicua protein expression levels are subject to posttranscriptional regulation by signaling downstream of two RTKs - EGFR and Torso (that propagate their signals through Ras-Raf-MAPK signaling cascade) and MAPK-mediated phosphorylation promotes capicua degradation. Capicua has been shown to act as a transcriptional repressor through interaction with a co-repressor Groucho. Moreover, Bantam which is a Hippo pathway player has recently been shown to acts in a feedback loop to limit Capicua expression for regulating EGFR signaling. Capicua is well conserved in mammals, and mediates RTK-dependent responses that are linked to cell proliferation, cell differentiation and cancer. Capicua can interact with ATXN1 as well as ATXN1L, and has been shown to be involved in Spinocerebellar Ataxia Type 1 (SCA-1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for Capicua Antibody (NB110-59906): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Capicua Antibody (NB110-59906) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-59906AF405
Alexa Fluor 488 Labeled NB110-59906AF488
Alexa Fluor 647 Labeled NB110-59906AF647
Alexa Fluor 700 Labeled NB110-59906AF700
Biotin Labeled NB110-59906B
DyLight 405 Labeled NB110-59906V
DyLight 488 Labeled NB110-59906G
DyLight 550 Labeled NB110-59906R
DyLight 650 Labeled NB110-59906C
FITC Labeled NB110-59906F
HRP Labeled NB110-59906H

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Bioinformatics

Gene Symbol CIC
Entrez
Uniprot

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