MID1 Antibody

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Western Blot: MID1 Antibody [NBP1-26612] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 1 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

MID1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 617 and 667 of human midline 1 using the numbering given in entry NP_000372.1 (GeneID 4281).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MID1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
Application Notes
This antibody is useful for western blot. It is not recommended for immunoprecipitation.
Publications
Read Publication using NBP1-26612.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MID1 Antibody

  • EC 6.3.2.-
  • FLJ57031
  • FLJ58683
  • FLJ76288
  • FXYGBBB1
  • Midin
  • midline 1 (Opitz/BBB syndrome)
  • RNF59MIDIN
  • TRIM18mouse, homolog of

Background

Defects in the Midline-1 (MID1) gene are the cause of Opitz syndrome, an X-linked recessive disorder characterized by developmental defects. MID1 is a microtubule-associated protein (MAP) with E3 ubiquitin ligase activity. MID1 has been shown to target protein phosphatases 2A (PP2Ac) on microtubules for degradation. MID1 association with PP2A has been proposed to regulate microtubule stabilization for cellular processes such as cell division and migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MID1 Antibody (NBP1-26612)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional MID1 Products

Bioinformatics Tool for MID1 Antibody (NBP1-26612)

Discover related pathways, diseases and genes to MID1 Antibody (NBP1-26612). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for MID1 Antibody (NBP1-26612)

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Pathways for MID1 Antibody (NBP1-26612)

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Research Areas for MID1 Antibody (NBP1-26612)

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Blogs on MID1.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

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Bioinformatics

Gene Symbol MID1
Entrez
Uniprot