TRIM21/SSA1 Antibody

Images

 
Western Blot: TRIM21/SSA1 Antibody [NBP1-87122] - Analysis in control (vector only transfected HEK293T lysate) and TRIM21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunocytochemistry/ Immunofluorescence: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human cell line HUVEC TERT2 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in lymphoid cells.
Western Blot: TRIM21/SSA1 Antibody [NBP1-87122] - Analysis in human cell line CAPAN-2.
Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human kidney shows moderate cytoplasmic and stronger nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human placenta shows strong cytoplasmic immunoreactivity in monocytes, as well as moderate positivity in trophoblast.
Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human skeletal muscle shows only weak positivity in striated muscle fibers as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TRIM21/SSA1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM21
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TRIM21/SSA1 Recombinant Protein Antigen (NBP1-87122PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TRIM21/SSA1 Antibody

  • 52 kDa ribonucleoprotein autoantigen Ro/SS-A
  • 52 kDa Ro/SSA
  • E3 ubiquitin-protein ligase TRIM21
  • EC 6.3.2.-
  • rhTRIM21
  • RNF81
  • RNF81RING finger protein 81
  • ro(SS-A)
  • Ro52
  • RO52SS-A
  • Sicca syndrome antigen A
  • Sjoegren syndrome type A antigen
  • Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)
  • SSA1
  • SSA1SSA
  • TRIM21
  • tripartite motif containing 21,52 kDa Ro protein
  • tripartite motif-containing 21
  • Tripartite motif-containing protein 21

Background

SSA1 encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TRIM21/SSA1 Antibody (NBP1-87122)

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Bioinformatics

Gene Symbol TRIM21