TRIM21/SSA1 Antibody

Western Blot: TRIM21/SSA1 Antibody [NBP1-87122] - Analysis in control (vector only transfected HEK293T lysate) and TRIM21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human cell line HUVEC TERT2 shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in lymphoid cells.

Western Blot: TRIM21/SSA1 Antibody [NBP1-87122] - Analysis in human cell line CAPAN-2.

Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human kidney shows moderate cytoplasmic and stronger nuclear positivity in cells in tubules.

Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human placenta shows strong cytoplasmic immunoreactivity in monocytes, as well as moderate positivity in trophoblast.

Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-87122] - Staining of human skeletal muscle shows only weak positivity in striated muscle fibers as expected.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TRIM21/SSA1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM21
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TRIM21/SSA1 Recombinant Protein Antigen (NBP1-87122PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TRIM21/SSA1 Antibody

• 52 kDa ribonucleoprotein autoantigen Ro/SS-A
• 52 kDa Ro/SSA
• E3 ubiquitin-protein ligase TRIM21
• EC 6.3.2.-
• rhTRIM21
• RNF81
• RNF81RING finger protein 81
• ro(SS-A)
• Ro52
• RO52SS-A
• Sicca syndrome antigen A
• Sjoegren syndrome type A antigen
• Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)
• SSA1
• SSA1SSA
• TRIM21
• tripartite motif containing 21,52 kDa Ro protein
• tripartite motif-containing 21
• Tripartite motif-containing protein 21

Background

SSA1 encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM21