Cadherin-11 Antibody - BSA Free

Immunohistochemistry-Frozen: Cadherin-11 Antibody [NBP2-15661] - Frozen-sectioned adult mouse cerebellum. Green: CDH11 stained by CDH11 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: Cadherin-11 Antibody [NBP2-15661] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Cadherin-11 antibody (NBP2-15661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: Cadherin-11 Antibody [NBP2-15661] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Cadherin-11 antibody (NBP2-15661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, RM, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cadherin-11 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cadherin-11. The exact sequence is proprietary.
• Localization: Cell membrane
• Predicted Species: Rat (100%), Porcine (99%), Bovine (100%), Rhesus Macaque (99%), Chicken (96%), Xenopus (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDH11
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (92%), Zebrafish (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Cadherin-11 Antibody - BSA Free

• CAD11OSF-4
• cadherin 11, type 2, OB-cadherin (osteoblast)
• Cadherin11
• Cadherin-11
• CDH11
• CDHOB
• OB
• OB-Cadherin
• Osteoblast cadherin

Background

This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cadherin-11 Antibody (NBP2-15661)(2)

Publications using NBP2-15661

• Han H, Xing J, Chen W et al. Fluorinated polyamidoamine dendrimer-mediated miR-23b delivery for the treatment of experimental rheumatoid arthritis in rats Nature communications 2023-02-20 [PMID: 36805456] (IHC, Mouse)
• Hosein A N, Huang H et al. Cellular heterogeneity during mouse pancreatic ductal adenocarcinoma progression at single-cell resolution. JCI Insight 2019-07-23 [PMID: 31335328] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: CDH11
• Uniprot:
  • P55287(Human)