BubR1 Antibody (8G1)

Images

 
Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.
Immunocytochemistry/ Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, ...read more
Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - An intracellular stain was performed on HeLa cells with BubR1 Antibody (8G1)NB100-353C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more
Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using BubR1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IP, CyTOF-ready
Clone
8G1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

BubR1 Antibody (8G1) Summary

Immunogen
A portion of human BubR1 tagged at the N-terminus with pMAL (residues 1-350). [UniProt# O60566]
Localization
Nuclear
Specificity
NB100-353 is specific for hBUBR1. There should be no cross-reactivity with hBUB1.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BUB1B
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:5000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
This BubR1 (8G1) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot. By WB, this antibody recognizes a band at ~120 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
BubR1 Lysate (NBL1-08057)
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NB100-353 in the following applications:

  • 2 publications
  • WB
    2 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for BubR1 Antibody (8G1)

  • Bub1A
  • BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
  • budding uninhibited by benzimidazoles 1 homolog beta (yeast)
  • EC 2.7.11.1
  • hBUBR1
  • MAD3/BUB1-related protein kinase
  • MAD3LBUB1beta
  • Mitotic checkpoint kinase MAD3L
  • Protein SSK1
  • SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Background

BubR1 (budding uninhibited by benzimidazole-related 1 or mitotic checkpoint serine/threonine-protein kinase BUB1 beta) is an essential component of mitotic checkpoint/control complex which is required for normal mitotic progression and any defects in this complex may result in chromosomal instability/aberrant mitosis. This evolutionarily conserved and crucial system of self-control in cell cycle warrants high quality of chromosomal segregation by postponing manifestation of anaphase in all chromosomes which are incorrectly bi-oriented to mitotic spindles. One of its checkpoint functions is to prevent the activity of anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity; and the other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. BubR1 is required for kinetochore localization of CENPE and it negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. BubR1 is also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest and thus it behaves as a tumor suppressior. Defects in BubR1 have been associated with tumor formation and are the underlying cause of premature chromatid separation trait (PCS) as well as mosaic variegated aneuploidy syndrome type 1 (MVA1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BubR1 Antibody (NB100-353)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(2)
WB
(2)
All Applications
Filter By Species
Human
(4)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BubR1 Antibody (NB100-353) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-353AF405
Alexa Fluor 488 Labeled NB100-353AF488
Alexa Fluor 647 Labeled NB100-353AF647
Alexa Fluor 700 Labeled NB100-353AF700
Allophycocyanin Labeled NB100-353APC
Biotin Labeled NB100-353B
DyLight 350 Labeled NB100-353UV
DyLight 405 Labeled NB100-353V
DyLight 488 Labeled NB100-353G
DyLight 550 Labeled NB100-353R
DyLight 650 Labeled NB100-353C
DyLight 680 Labeled NB100-353FR
DyLight 755 Labeled NB100-353IR
FITC Labeled NB100-353F
HRP Labeled NB100-353H
PE Labeled NB100-353PE

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Bioinformatics

Gene Symbol BUB1B