BubR1 Antibody (8G1)


Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.
Immunocytochemistry/ Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, ...read more
Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - An intracellular stain was performed on HeLa cells with BubR1 Antibody (8G1)NB100-353C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more
Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using BubR1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ...read more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IP, CyTOF-ready
1.0 mg/ml

Order Details

BubR1 Antibody (8G1) Summary

A portion of human BubR1 tagged at the N-terminus with pMAL (residues 1-350). [UniProt# O60566]
NB100-353 is specific for hBUBR1. There should be no cross-reactivity with hBUB1.
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:2000-1:5000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
This BubR1 (8G1) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot. By WB, this antibody recognizes a band at ~120 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
BubR1 Overexpression Lysate
HeLa Whole Cell Lysate
Read Publications using
NB100-353 in the following applications:

  • 3 publications
  • WB
    3 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for BubR1 Antibody (8G1)

  • Bub1A
  • BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
  • budding uninhibited by benzimidazoles 1 homolog beta (yeast)
  • EC
  • hBUBR1
  • MAD3/BUB1-related protein kinase
  • MAD3LBUB1beta
  • Mitotic checkpoint kinase MAD3L
  • Protein SSK1
  • SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta


BubR1 (budding uninhibited by benzimidazole-related 1 or mitotic checkpoint serine/threonine-protein kinase BUB1 beta) is an essential component of mitotic checkpoint/control complex which is required for normal mitotic progression and any defects in this complex may result in chromosomal instability/aberrant mitosis. This evolutionarily conserved and crucial system of self-control in cell cycle warrants high quality of chromosomal segregation by postponing manifestation of anaphase in all chromosomes which are incorrectly bi-oriented to mitotic spindles. One of its checkpoint functions is to prevent the activity of anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity; and the other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. BubR1 is required for kinetochore localization of CENPE and it negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. BubR1 is also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest and thus it behaves as a tumor suppressior. Defects in BubR1 have been associated with tumor formation and are the underlying cause of premature chromatid separation trait (PCS) as well as mosaic variegated aneuploidy syndrome type 1 (MVA1).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, Flow, ICC/IF, PA
Species: Hu, Mu
Applications: WB, ICC/IF, PLA
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, KD
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, MiAr
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF
Species: Hu, Mu, Rt, Po, Bv, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
Species: Hu
Applications: ICC/IF (-), WB, Flow, IHC, CyTOF-ready
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IP, CyTOF-ready

Publications for BubR1 Antibody (NB100-353)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-353 Applications Species
Kiseljak-Vassiliades K, Zhang Y, Kar A et al. Elucidating the Role of the Maternal Embryonic Leucine Zipper Kinase (MELK) in Adrenocortical Carcinoma Endocrinology May 21 2018 [PMID: 29790920] (WB, Human) WB Human
Martini S, Soliman T, Gobbi G, Mirandola P et al. PKC epsilon Controls Mitotic Progression by Regulating Centrosome Migration and Mitotic Spindle Assembly Mol. Cancer Res. 2017 Oct 11 [PMID: 29021232] (ICC/IF, Human) ICC/IF Human
Silio V, Redondo-Munoz J, Carrera AC. Phosphoinositide 3-kinase beta regulates chromosome segregation in mitosis Mol Biol Cell 2012 Dec [PMID: 23051731] (ICC/IF, Human) ICC/IF Human
Deeraksa A, Pan J, Sha Y et al. Plk1 is upregulated in androgen-insensitive prostate cancer cells and its inhibition leads to necroptosis Oncogene 2012 Aug 13 [PMID: 22890325] (WB, Human) WB Human
De Rycker M, Rigoreau L, Dowding S et al. A high-content, cell-based screen identifies micropolyin, a new inhibitor of microtubule dynamics. Chem Biol Drug Des 2009 Jun [PMID: 19635051] (ICC/IF, Human) ICC/IF Human
Bohers E, Sarafan-Vasseur N, Drouet A et al. Gradual reduction of BUBR1 protein levels results in premature sister-chromatid separation then in aneuploidy. Hum Genet 2008 Dec [PMID: 18932004] (WB, Human) WB Human
Trachana, V et al. Dido disruption leads to centrosome amplification and mitotic checkpoint defects compromising chromosome stability. Proc Natl Acad Sci U S A;104(8):2691-6. 2007 Feb 20. [PMID: 17299043]
Chan, GK et al. Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC. J Cell Biol;146(5):941-54. 1999 Sep 6. [PMID: 10477750]

Reviews for BubR1 Antibody (NB100-353) (0)

There are no reviews for BubR1 Antibody (NB100-353). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BubR1 Antibody (NB100-353) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-353AF350
Alexa Fluor 405 NB100-353AF405
Alexa Fluor 488 NB100-353AF488
Alexa Fluor 532 NB100-353AF532
Alexa Fluor 594 NB100-353AF594
Alexa Fluor 647 NB100-353AF647
Alexa Fluor 700 NB100-353AF700
Alexa Fluor 750 NB100-353AF750
Allophycocyanin NB100-353APC
Biotin NB100-353B
DyLight 350 NB100-353UV
DyLight 405 NB100-353V
DyLight 488 NB100-353G
DyLight 550 NB100-353R
DyLight 594 NB100-353DL594
DyLight 650 NB100-353C
DyLight 680 NB100-353FR
DyLight 755 NB100-353IR
FITC NB100-353F
HRP NB100-353H
Janelia Fluor 549 NB100-353JF549
Janelia Fluor 646 NB100-353JF646
PE NB100-353PE

Additional BubR1 Products

Bioinformatics Tool for BubR1 Antibody (NB100-353)

Discover related pathways, diseases and genes to BubR1 Antibody (NB100-353). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BubR1 Antibody (NB100-353)

Discover more about diseases related to BubR1 Antibody (NB100-353).

Pathways for BubR1 Antibody (NB100-353)

View related products by pathway.

PTMs for BubR1 Antibody (NB100-353)

Learn more about PTMs related to BubR1 Antibody (NB100-353).

Research Areas for BubR1 Antibody (NB100-353)

Find related products by research area.

Blogs on BubR1

There are no specific blogs for BubR1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our BubR1 Antibody (8G1) and receive a gift card or discount.


Gene Symbol BUB1B