BubR1 Antibody (8G1) [Alexa Fluor® 405]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
8G1
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 405

Order Details

BubR1 Antibody (8G1) [Alexa Fluor® 405] Summary

Immunogen
A portion of human BubR1 tagged at the N-terminus with pMAL (residues 1-350). [UniProt# O60566]
Localization
Nuclear
Specificity
NB100-353 is specific for hBUBR1. There should be no cross-reactivity with hBUB1.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BUB1B
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
This BubR1 (8G1) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot. By WB, this antibody recognizes a band at ~120 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for BubR1 Antibody (8G1) [Alexa Fluor® 405]

  • Bub1A
  • BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
  • budding uninhibited by benzimidazoles 1 homolog beta (yeast)
  • EC 2.7.11.1
  • hBUBR1
  • MAD3/BUB1-related protein kinase
  • MAD3LBUB1beta
  • Mitotic checkpoint kinase MAD3L
  • Protein SSK1
  • SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BubR1 Antibody (NB100-353AF405) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-353AF350
Alexa Fluor 405 NB100-353AF405
Alexa Fluor 488 NB100-353AF488
Alexa Fluor 532 NB100-353AF532
Alexa Fluor 594 NB100-353AF594
Alexa Fluor 647 NB100-353AF647
Alexa Fluor 700 NB100-353AF700
Alexa Fluor 750 NB100-353AF750
Allophycocyanin NB100-353APC
Biotin NB100-353B
DyLight 350 NB100-353UV
DyLight 405 NB100-353V
DyLight 488 NB100-353G
DyLight 550 NB100-353R
DyLight 594 NB100-353DL594
DyLight 650 NB100-353C
DyLight 680 NB100-353FR
DyLight 755 NB100-353IR
FITC NB100-353F
HRP NB100-353H
Janelia Fluor 549 NB100-353JF549
Janelia Fluor 646 NB100-353JF646
PE NB100-353PE

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Bioinformatics

Gene Symbol BUB1B
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