BubR1 Antibodies
We offer BubR1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each BubR1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our BubR1 Antibodies can be used in a variety of model species: Human, Monkey, Mouse. Use the list below to choose the BubR1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our BubR1 polyclonal antibodies and browse our BubR1 monoclonal antibody catalog.
Alternate Names for BubR1 Antibodies
anti-BubR1 antibody, anti-BUB1B antibody, anti-Bub1A antibody, anti-BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta antibody, anti-budding uninhibited by benzimidazoles 1 homolog beta (yeast) antibody, anti-EC 2.7.11.1 antibody, anti-hBUBR1 antibody, anti-MAD3/BUB1-related protein kinase antibody, anti-MAD3LBUB1beta antibody, anti-Mitotic checkpoint kinase MAD3L antibody, anti-Protein SSK1 antibody, anti-SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta antibody
10 Results for "bubr1" in Primary Antibodies
![Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Western-Blot-NB100-353-img0006.jpg "Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.")
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Immunocytochemistry-Immunofluorescence-NB100-353-img0005.jpg)
![Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - An intracellular stain was performed on HeLa cells with BubR1 Antibody (8G1)NB100-353C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Flow-Cytometry-NB100-353-img0008.jpg)
![Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using BubR1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Flow-Cytometry-NB100-353-img0007.jpg)
![Western Blot: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Western-Blot-NBP1-47680-img0002.jpg "Western Blot: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.")
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (OTI6E5) [NBP1-47680] - BubR1 Antibody (6E5) [NBP1-47680] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunocytochemistry-Immunofluorescence-NBP1-47680-img0012.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded prostate tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0009.jpg)
![Flow Cytometry: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells transfected with either pCMV6-ENTRY BubR1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-BubR1 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Flow-Cytometry-NBP1-47680-img0011.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0003.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0004.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0005.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0006.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded endometrium tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0007.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded pancreas tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0008.jpg)
![Western Blot: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Western-Blot-NBP2-70314-img0001.jpg "Western Blot: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.")
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70314-img0003.jpg)
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded pancreas tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0009.jpg)
![Flow Cytometry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - HEK293T cells transfected with either pCMV6-ENTRY BubR1.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-BubR1 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70314-img0002.jpg)
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0004.jpg)
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0005.jpg)
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0006.jpg)
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0007.jpg)
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded endometrium tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0008.jpg)
![Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded prostate tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-OTI6E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70314-img0010.jpg)
![Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.Lane 1: BUB1B transfected lysate(119.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Western-Blot-H00000701-M02-img0012.jpg "Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.Lane 1: BUB1B transfected lysate(119.5 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of monoclonal antibody to BUB1B on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Immunohistochemistry-Paraffin-H00000701-M02-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi ( Cat # H00000701-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody (M02) clone 2G5 (Cat # H00000701-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Western-Blot-H00000701-M02-img0009.jpg)
![Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - BUB1B monoclonal antibody (M02), clone 2G5 Analysis of BUB1B expression in Hela S3 NE.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Western-Blot-H00000701-M02-img0011.jpg)
![Proximity Ligation Assay: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Proximity-Ligation-Assay-H00000701-M02-img0006.jpg)
![Sandwich ELISA: BubR1 Antibody (2G5) [H00000701-M02] - Detection limit for recombinant GST tagged BUB1B is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Sandwich-ELISA-H00000701-M02-img0010.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Human BubR1 on HeLa whole cell lysate using NB100-579. BubR1 was also immunoprecipitated using another affinity purified rabbit anti-BubR1 antibody to a different epitope.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0002.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0001.jpg)
![Western Blot: BubR1 Antibody (S05-3G7) [NBP3-19840] - Western blot detection of BubR1 in CHO-K1, Ramos cell lysates using NBP3-19840 (1:1000 diluted). Predicted band size: 120kDa. Observed band size: 120kDa.](http://images.novusbio.com/fullsize/BubR1-Antibody-S05-3G7-Western-Blot-NBP3-19840-img0002.jpg "Western Blot: BubR1 Antibody (S05-3G7) [NBP3-19840] - Western blot detection of BubR1 in CHO-K1, Ramos cell lysates using NBP3-19840 (1:1000 diluted). Predicted band size: 120kDa. Observed band size: 120kDa.")
![Immunohistochemistry-Paraffin: BubR1 Antibody (S05-3G7) [NBP3-19840] - Immunohistochemistry of BubR1 in paraffin-embedded Human lung cancer tissue using NBP3-19840 at dilution 1/50](http://images.novusbio.com/fullsize/BubR1-Antibody-S05-3G7-Immunohistochemistry-Paraffin-NBP3-19840-img0001.jpg)
![Western Blot: BubR1 Antibody - C-terminus [NBP3-30805] - Immunoprecipitation/Western blot analysis using Bub1b-CT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.](http://images.novusbio.com/fullsize/nbp3-30805_sheep-bubr1-pab-c-terminus-811202420592120.jpg "Western Blot: BubR1 Antibody - C-terminus [NBP3-30805] - Immunoprecipitation/Western blot analysis using Bub1b-CT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.")
![Western Blot: BubR1 Antibody [NBP3-18818] - Immunoprecipitation/ Western blot analysis on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flow cytometry](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NBP3-18818-img0001.jpg "Western Blot: BubR1 Antibody [NBP3-18818] - Immunoprecipitation/ Western blot analysis on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flow cytometry")
![Western Blot: BubR1 Antibody - N-terminal [NBP3-30806] - Immunoprecipitation/Western blot analysis using Bub1b-NT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.](http://images.novusbio.com/fullsize/nbp3-30806_sheep-bubr1-pab-n-terminal-811202420594239.jpg "Western Blot: BubR1 Antibody - N-terminal [NBP3-30806] - Immunoprecipitation/Western blot analysis using Bub1b-NT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.")
![Western Blot: BubR1 Antibody (7H4) [NBP3-15207] - Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate. All lanes: BubR1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 120, 106, 122 KDa. Observed band size: 125 KDa.](http://images.novusbio.com/fullsize/BubR1-Antibody-7H4-Western-Blot-NBP3-15207-img0002.jpg "Western Blot: BubR1 Antibody (7H4) [NBP3-15207] - Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate. All lanes: BubR1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 120, 106, 122 KDa. Observed band size: 125 KDa.")
![Flow Cytometry: BubR1 Antibody (7H4) [NBP3-15207] - Overlay histogram showing Hela cells stained with BubR1 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/BubR1-Antibody-7H4-Flow-Cytometry-NBP3-15207-img0001.jpg)