Immunocytochemistry/ Immunofluorescence: BRD4 Antibody [NBP1-86640] - Staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Simple Western: BRD4 Antibody [NBP1-86640] - Simple Western lane view shows a specific band for BRD4 in 0.2 mg/ml of U-251MG sp lysate. This experiment was performed under reducing conditions using the 66-440 kDa ...read more
Simple Western: BRD4 Antibody [NBP1-86640] - Electropherogram image(s) of corresponding Simple Western lane view. BRD4 antibody was used at 1:60 dilution on U-251MG sp lysates(s).
Simple Western, ICC/IF, IHC, IHC-P
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
This antibody was developed against Recombinant Protein corresponding to amino acids:PQPAKPQQVIQHHHSPRHHKSDPYSTGHLREAPSPLMIHSPQMSQFQSLTHQSPPQQNVQPKKQELRAASVVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPKHPESIKAPVHLPQRPEMKPVDVGRPVIRPPEQNAPPP
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Immunogen affinity purified
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NUT - A Protein Coding Gene The NUT gene is found on chromosome 15q14 and encodes for the NUT protein which is a key component of the RNA polymerase II Mediator complex. This multi-subunit assembly is required for all RNA pol II-dependent transcriptional activation, coordinating... Read full blog post.