Immunocytochemistry/ Immunofluorescence: BRD4 Antibody [NBP1-86640] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human tonsil shows moderate to strong nuclear positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Simple Western: BRD4 Antibody [NBP1-86640] - Simple Western lane view shows a specific band for BRD4 in 0.2 mg/ml of U-251MG sp lysate. This experiment was performed under reducing conditions using the 66-440 kDa ...read more
Simple Western: BRD4 Antibody [NBP1-86640] - Electropherogram image(s) of corresponding Simple Western lane view. BRD4 antibody was used at 1:60 dilution on U-251MG sp lysates(s).
This antibody was developed against Recombinant Protein corresponding to amino acids: PQPAKPQQVIQHHHSPRHHKSDPYSTGHLREAPSPLMIHSPQMSQFQSLTHQSPPQQNVQPKKQELRAASVVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPKHPESIKAPVHLPQRPEMKPVDVGRPVIRPPEQNAPPP
Specificity of human BRD4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Rat (94%). Backed by our 100% Guarantee.
Immunogen affinity purified
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ChIP reported in the literature (PMID:25616107). ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin HIER pH6 retrieval is recommended. Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
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