BRD4 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: BRD4 Antibody [NBP1-86640] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Simple Western: BRD4 Antibody [NBP1-86640] - Simple Western lane view shows a specific band for BRD4 in 0.2 mg/ml of U-251MG sp lysate. This experiment was performed under reducing conditions using the 66-440 kDa ...read more
Simple Western: BRD4 Antibody [NBP1-86640] - Electropherogram image(s) of corresponding Simple Western lane view. BRD4 antibody was used at 1:60 dilution on U-251MG sp lysates(s).

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

BRD4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:PQPAKPQQVIQHHHSPRHHKSDPYSTGHLREAPSPLMIHSPQMSQFQSLTHQSPPQQNVQPKKQELRAASVVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPKHPESIKAPVHLPQRPEMKPVDVGRPVIRPPEQNAPPP
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BRD4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 1:60
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
BRD4 Protein (NBP1-86640PEP)
Publications
Read Publications using
NBP1-86640 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25616107).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for BRD4 Antibody

  • bromodomain containing 4
  • bromodomain-containing 4
  • CAPchromosome-associated protein
  • HUNK1bromodomain-containing protein 4
  • HUNKI
  • MCAP
  • Protein HUNK1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BRD4 Antibody (NBP1-86640)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ChIP.


Filter By Application
ChIP
(1)
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Human
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for BRD4 Antibody (NBP1-86640) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for BRD4 Antibody (NBP1-86640)

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Blogs on BRD4.

Understanding the relationship between NUT and BET proteins in NMC
NUT has been found to fuse with bromodomain-containing proteins 3 and 4 (BRD3 and BRD4) in NUT midline carcinoma (NMC), a very rare, extremely aggressive, and genetically defined human cancer. NMC has recently been designated as a sub classificati...  Read full blog post.

NUT - A Protein Coding Gene
The NUT gene is found on chromosome 15q14 and encodes for the NUT protein which is a key component of the RNA polymerase II Mediator complex. This multi-subunit assembly is required for all RNA pol II-dependent transcriptional activation, coordinating...  Read full blog post.

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Bioinformatics

Gene Symbol BRD4