SGPL1 Antibody [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, HT‑29 human colon adenocarcinoma cell line, and HT‑29 tumor tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SGPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SGPL1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SGPL1 at approximately 60 kDa (as indicated) using 50 µg/mL of Goat Anti-Human SGPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5535) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SGPL1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SGPL1
  Lys342-His568
  Accession # O95470
• Specificity: Detects human SGPL1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SGPL1
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SGPL1 Antibody [Unconjugated]

• EC 4.1.2.27
• FLJ13811
• hSPL
• KIAA1252
• S1PL
• SGPL1
• Sphingosine-1-phosphate aldolase
• sphingosine-1-phosphate lyase 1
• SPL
• SPLSPL 1
• SP-lyase 1
• SP-lyase

Background

SGPL1 (Sphingosine-1-phosphate lyase; also hSPL) is a 60‑63 kDa member of the SGPL1 subfamily, group II decarboxylase family of enzymes. It is very widely expressed, and particularly concentrated in cells undergoing rapid turnover. SGPL1 is found in the ER, and cleaves phosphorylated sphingoid bases into fatty aldehydes and phosphoethanolamine. This regulates a diversity of cellular processes such as apoptosis and T cell migration. Human SGPL1 is 568 amino acids (aa) in length. It is a type III transmembrane protein (luminal N-terminus with no signal sequence) that contains a 507 aa cytoplasmic domain (aa 62‑568). This domain contains a vitamin B6 binding site (aa 344‑364) that is part of a large catalytic region (aa 116‑506). Over aa 342‑568, human SGPL1 shares 86% aa identity with mouse SGPL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SGPL1 Antibody (AF5535)(9)

Publications using AF5535

• AS De la Garz, SA Moore, J Zamora-Pin, EP Hoffman, K Mistry, A Kumar, JB Strober, P Zhao, JH Suh, JD Saba Sphingosine Phosphate Lyase Is Upregulated in Duchenne Muscular Dystrophy, and Its Inhibition Early in Life Attenuates Inflammation and Dystrophy in Mdx Mice International Journal of Molecular Sciences, 2022-07-08;23(14):. 2022-07-08 [PMID: 35886926] (IHC, Human)
• P Zhao, GB Tassew, JY Lee, B Oskouian, DP Muñoz, JB Hodgin, GL Watson, F Tang, JY Wang, J Luo, Y Yang, SM King, RM Krauss, N Keller, JD Saba Efficacy of AAV9-mediated SGPL1 gene transfer in a mouse model of S1P lyase insufficiency syndrome JCI Insight, 2021-04-22;0(0):. 2021-04-22 [PMID: 33755599] (Western Blot, Human)
• K Takahashi, M Fujiya, H Konishi, Y Murakami, T Iwama, T Sasaki, T Kunogi, A Sakatani, K Ando, N Ueno, S Kashima, K Moriichi, H Tanabe, T Okumura Heterogenous Nuclear Ribonucleoprotein H1 Promotes Colorectal Cancer Progression through the Stabilization of mRNA of Sphingosine-1-Phosphate Lyase 1 Int J Mol Sci, 2020-06-25;21(12):. 2020-06-25 [PMID: 32630435] (Western Blot, Human)
• VA Taylor, HK Stone, MP Schuh, X Zhao, KD Setchell, E Erkan Disarranged Sphingolipid Metabolism From Sphingosine-1-Phosphate Lyase Deficiency Leads to Congenital Nephrotic Syndrome Kidney Int Rep, 2019-08-07;4(12):1763-1769. 2019-08-07 [PMID: 31844815] (IHC, Human)
• D Yamaguchi, T Imaizumi, K Yagi, Y Matsumoto, T Nakashima, A Hirose, N Kashima, Y Nosaka, T Hamada, K Okawa, Y Nishiya, K Kubo Nicotinamide phosphoribosyltransferase is a molecular target of potent anticancer agents identified from phenotype-based drug screening Sci Rep, 2019-05-23;9(1):7742. 2019-05-23 [PMID: 31123329] (Western Blot, Human)
• S Lovric, S Goncalves, HY Gee, B Oskouian, H Srinivas, WI Choi, S Shril, S Ashraf, W Tan, J Rao, M Airik, D Schapiro, DA Braun, CE Sadowski, E Widmeier, T Jobst-Schw, JM Schmidt, V Girik, G Capitani, JH Suh, N Lachaussée, C Arrondel, J Patat, O Gribouval, M Furlano, O Boyer, A Schmitt, V Vuiblet, S Hashmi, R Wilcken, FP Bernier, AM Innes, JS Parboosing, RE Lamont, JP Midgley, N Wright, J Majewski, M Zenker, F Schaefer, N Kuss, J Greil, T Giese, K Schwarz, V Catheline, D Schanze, I Franke, Y Sznajer, AS Truant, B Adams, J Désir, R Biemann, Y Pei, E Ars, N Lloberas, A Madrid, VR Dharnidhar, AM Connolly, MC Willing, MA Cooper, RP Lifton, M Simons, H Riezman, C Antignac, JD Saba, F Hildebrand Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency J. Clin. Invest., 2017-02-06;127(3):912-928. 2017-02-06 [PMID: 28165339] (IHC, Western Blot, Mouse, Rat)
• Abu El-Asrar , Ahmed M, Nawaz , Mohd Imt, Mohammad , Ghulam, Siddiquei , Mohammad, Alam , Kaiser, Mousa , Ahmed, Opdenakker , Ghislain Expression of bioactive lysophospholipids and processing enzymes in the vitreous from patients with proliferative diabetic retinopathy. Lipids Health Dis, 2014-12-11;13(0):187. 2014-12-11 [PMID: 25496321] (Western Blot, Human)
• Gatfield J, Monnier L, Studer R, Bolli M, Steiner B, Nayler O Sphingosine-1-phosphate (S1P) displays sustained S1P1 receptor agonism and signaling through S1P lyase-dependent receptor recycling. Cell Signal, 2014-04-02;26(7):1576-88. 2014-04-02 [PMID: 24704119] (Western Blot, Human)
• Erkhembaatar L, Kotani T, Sumigama S, Tsuda H, Mano Y, Hua L, Hasegawa Y, Wang J, Sugiyama C, Nakahara T, Iwase A, Kikkawa F Increased expression of sphingosine kinase in the amnion during labor. Placenta, 2013-02-22;34(4):353-9. 2013-02-22 [PMID: 23462226] (IHC-P, Western Blot, Human)

Bioinformatics

• Gene Symbol: SGPL1
• Uniprot:
  • O95470()