SGPL1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, HT‑29 human colon adenocarcinoma cell line, and HT‑29 tumor tissue. PVDF membrane was probed ...read more
Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SGPL1 at approximately 60 kDa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

SGPL1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SGPL1
Lys342-His568
Accession # O95470
Specificity
Detects human SGPL1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SGPL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 50 ug/mL
Publications
Read Publications using
AF5535 in the following applications:

  • IHC
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SGPL1 Antibody [Unconjugated]

  • EC 4.1.2.27
  • FLJ13811
  • hSPL
  • KIAA1252
  • S1PL
  • SGPL1
  • Sphingosine-1-phosphate aldolase
  • sphingosine-1-phosphate lyase 1
  • SPL
  • SPLSPL 1
  • SP-lyase 1
  • SP-lyase

Background

SGPL1 (Sphingosine-1-phosphate lyase; also hSPL) is a 60‑63 kDa member of the SGPL1 subfamily, group II decarboxylase family of enzymes. It is very widely expressed, and particularly concentrated in cells undergoing rapid turnover. SGPL1 is found in the ER, and cleaves phosphorylated sphingoid bases into fatty aldehydes and phosphoethanolamine. This regulates a diversity of cellular processes such as apoptosis and T cell migration. Human SGPL1 is 568 amino acids (aa) in length. It is a type III transmembrane protein (luminal N-terminus with no signal sequence) that contains a 507 aa cytoplasmic domain (aa 62‑568). This domain contains a vitamin B6 binding site (aa 344‑364) that is part of a large catalytic region (aa 116‑506). Over aa 342‑568, human SGPL1 shares 86% aa identity with mouse SGPL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-30981
Species: Hu, Mu
Applications: WB, ICC/IF
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP1-32108
Species: Hu, Rt
Applications: WB, ICC/IF
NBP2-45897
Species: Hu
Applications: WB, IHC
NBP1-47791
Species: Hu, Mk
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
H00009200-R01
Species: Hu
NBP2-01650
Species: Hu, Mu, Mk
Applications: WB, Flow, IHC, IHC-P
NBP1-86644
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-86641
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IF
NB100-56487
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ICC/IF, IP
AF6386
Species: Hu
Applications: WB, IP
NBP1-32767
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00003990-R01
Species: Hu
NBP1-32870
Species: Hu, Ch
Applications: WB, IHC, IHC-P, IP
NBP1-32975
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
MAB25031
Species: Hu
Applications: WB, IHC, IP
NB600-232
Species: Hu, Mu
Applications: WB, ChIP, IHC-P, IP
MAB4077
Species: Hu
Applications: WB, IHC

Publications for SGPL1 Antibody (AF5535)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 3 of 3.
Publications using AF5535 Applications Species
S Lovric, S Goncalves, HY Gee, B Oskouian, H Srinivas, WI Choi, S Shril, S Ashraf, W Tan, J Rao, M Airik, D Schapiro, DA Braun, CE Sadowski, E Widmeier, T Jobst-Schw, JM Schmidt, V Girik, G Capitani, JH Suh, N Lachaussée, C Arrondel, J Patat, O Gribouval, M Furlano, O Boyer, A Schmitt, V Vuiblet, S Hashmi, R Wilcken, FP Bernier, AM Innes, JS Parboosing, RE Lamont, JP Midgley, N Wright, J Majewski, M Zenker, F Schaefer, N Kuss, J Greil, T Giese, K Schwarz, V Catheline, D Schanze, I Franke, Y Sznajer, AS Truant, B Adams, J Désir, R Biemann, Y Pei, E Ars, N Lloberas, A Madrid, VR Dharnidhar, AM Connolly, MC Willing, MA Cooper, RP Lifton, M Simons, H Riezman, C Antignac, JD Saba, F Hildebrand Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency J. Clin. Invest., 2017;127(3):912-928. 2017 [PMID: 28165339] (IHC,, Mouse) IHC Mouse
Abu El-Asrar, Ahmed M, Nawaz, Mohd Imt, Mohammad, Ghulam, Siddiquei, Mohammad, Alam, Kaiser, Mousa, Ahmed, Opdenakker, Ghislain Expression of bioactive lysophospholipids and processing enzymes in the vitreous from patients with proliferative diabetic retinopathy. Lipids Health Dis, 2014;13(0):187. 2014 [PMID: 25496321] (WB,, Human) WB Human
Erkhembaatar L, Kotani T, Sumigama S, Tsuda H, Mano Y, Hua L, Hasegawa Y, Wang J, Sugiyama C, Nakahara T, Iwase A, Kikkawa F Increased expression of sphingosine kinase in the amnion during labor. Placenta, 2013;34(4):353-9. 2013 [PMID: 23462226] (WB,, Human) WB Human

Reviews for SGPL1 Antibody (AF5535) (0)

There are no reviews for SGPL1 Antibody (AF5535). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SGPL1 Antibody (AF5535) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SGPL1 Products

Bioinformatics Tool for SGPL1 Antibody (AF5535)

Discover related pathways, diseases and genes to SGPL1 Antibody (AF5535). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SGPL1 Antibody (AF5535)

Discover more about diseases related to SGPL1 Antibody (AF5535).
 

Pathways for SGPL1 Antibody (AF5535)

View related products by pathway.

PTMs for SGPL1 Antibody (AF5535)

Learn more about PTMs related to SGPL1 Antibody (AF5535).

Blogs on SGPL1

There are no specific blogs for SGPL1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

LIPC RNAi
H00003990-R01-20nmol
SGPL1 RNAi
H00008879-R01-20nmol

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SGPL1 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol SGPL1
Entrez
Uniprot