BRD4 Antibody

Images

 
Western Blot: BRD4 Antibody [NBP1-18874] - Whole cell lysate (15 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD4 antibody used for WB at 0.1 ug/ml. Detection: ...read more
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB
Independent Antibodies: Western Blot: BRD4 Antibody [NBP1-18874] - Whole cell lysate from HeLa cells. BRD4 was also immunoprecipitated by another rabbit anti-BRD4 antibody.
Western Blot: BRD4 Antibody [NBP1-18874] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, MB231, HepG2, A549, SW620, SK-MEL28 (SK), and Ramos cells prepared using NETN lysis buffer. Antibody: Affinity Purified ...read more
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874] - FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

BRD4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1312 and 1362 of human bromodomain-containing protein 4 using the numbering given in entry NP_490597.1 (GeneID 23476).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BRD4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Control
BRD4 Knockout 293T Cell Lysate
Publications
Read Publications using NBP1-18874.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BRD4 Antibody

  • bromodomain containing 4
  • bromodomain-containing 4
  • CAPchromosome-associated protein
  • HUNK1bromodomain-containing protein 4
  • HUNKI
  • MCAP
  • Protein HUNK1

Background

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BRD4 Antibody (NBP1-18874)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for BRD4 Antibody (NBP1-18874). (Showing 1 - 1 of 1 FAQs).

  1. Why is BRD4 detected at around 200kd when it is a 152kd protein? Additionally, I noticed another company's antibody detects a protein of around 85kd.
    • Our antibody is detecting the full length BRD4 protein 1362 amino acids in length, which corresponds to approximately 200kDa. This is also referred to as the long isoform of BRD4. The 85kDa band that you mention being detected by another antibody would most likely correspond to the short isoform of BRD4 which is the alternative splice variant. The size of the short isoform is 722 amino acids which corresponds to approximately 83.45kDa.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional BRD4 Products

Bioinformatics Tool for BRD4 Antibody (NBP1-18874)

Discover related pathways, diseases and genes to BRD4 Antibody (NBP1-18874). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on BRD4.

Epigenetic Control of Autophagy
By Christina Towers, PhD. In the last 20 years, epigenetic regulation has become front and center for almost all fields of biology and its role in diseases like cancer and neurodegeneration are being heavily studied....  Read full blog post.

Understanding the relationship between NUT and BET proteins in NMC
NUT has been found to fuse with bromodomain-containing proteins 3 and 4 (BRD3 and BRD4) in NUT midline carcinoma (NMC), a very rare, extremely aggressive, and genetically defined human cancer. NMC has recently been designated as a sub classificati...  Read full blog post.

NUT - A Protein Coding Gene
The NUT gene is found on chromosome 15q14 and encodes for the NUT protein which is a key component of the RNA polymerase II Mediator complex. This multi-subunit assembly is required for all RNA pol II-dependent transcriptional activation, coordinating...  Read full blog post.

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Bioinformatics

Gene Symbol BRD4
Entrez
Uniprot