Home » BRD4 » BRD4 Antibodies » BRD4 Antibody [HRP]

BRD4 Antibody [HRP]

 Submit a Review

Images

 
There are currently no images for BRD4 Antibody (NBP1-18874H).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-18874AF350
Alexa Fluor 405
NBP1-18874AF405
Alexa Fluor 488
NBP1-18874AF488
Alexa Fluor 532
NBP1-18874AF532
Alexa Fluor 594
NBP1-18874AF594
Alexa Fluor 647
NBP1-18874AF647
Alexa Fluor 700
NBP1-18874AF700
Alexa Fluor 750
NBP1-18874AF750
DyLight 350
NBP1-18874UV
DyLight 405
NBP1-18874V
DyLight 488
NBP1-18874G
DyLight 550
NBP1-18874R
DyLight 594
NBP1-18874DL594
DyLight 650
NBP1-18874C
DyLight 680
NBP1-18874FR
DyLight 755
NBP1-18874IR
FITC
NBP1-18874F
Janelia Fluor 549
NBP1-18874JF549
Janelia Fluor 646
NBP1-18874JF646
Unconjugated
NBP1-18874
100% Guarantee on every product

BRD4 Antibody [HRP] Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1312 and 1362 of human bromodomain-containing protein 4 using the numbering given in entry NP_490597.1 (GeneID 23476).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BRD4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Control
BRD4 Knockout 293T Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for BRD4 Antibody [HRP]

  • BRD4
  • bromodomain containing 4
  • bromodomain-containing 4
  • CAPchromosome-associated protein
  • HUNK1
  • HUNK1bromodomain-containing protein 4
  • HUNKI
  • MCAP
  • Protein HUNK1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Catalase Antibody
NBP2-24916
Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
(1 Review)
(6 Publications)
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
Recombinant Human
TFF2 Protein
NBP2-35058
Recombinant Human TFF2 Protein
Species: Hu
Vanilloid R1/TRPV1
Antibody
NB100-1617
Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NB100-1617] - CGRP and TRPV1 are co-expressed in most cultured trigeminal ganglion neurons. (A and C) CGRP expression in d2 trigeminal ganglion neurons plated on poly-d-lysine-coated coverslips. (B and D) The same culture shown in panel A or C costained for TRPV1 expression. Magnification bar = 200m (A and B) or 40m (C and D)
Immunohistochemistry: Vanilloid R1/TRPV1 Antibody [NB100-1617] - TRPV1 Antibody [NB100-1617] - Dorsal Root Ganglion
Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NB100-1617] - TRPV1 staining in rat trigeminal ganglion. Image courtesy of Dr. Norman Capra, University of Maryland
(18 Publications)
Species: Hu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
CRAT Antibody
NBP1-86616
Western Blot: CRAT Antibody [NBP1-86616] - Analysis using Anti-CRAT antibody NBP1-86616 (A) shows similar pattern to independent antibody NBP1-86615 (B).
Immunohistochemistry-Paraffin: CRAT Antibody [NBP1-86616] - Analysis in human testis and lymph node tissues. Corresponding CRAT RNA-seq data are presented for the same tissues.
Western Blot: CRAT Antibody [NBP1-86616] - Analysis in human cell line TD47D.
Species: Hu
Applications: WB, IHC, IHC-P
GLYAT Antibody
NBP2-58917
Western Blot: GLYAT Antibody [NBP2-58917] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.
Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human skeletal muscle.
Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human duodenum shows low expression as expected.
Species: Hu
Applications: WB, IHC, IHC-P
ErbB2/Her2
[Unconjugated]
1129-ER
1 μg/lane of Recombinant Human ErbB2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 125-130 kDa and  220-250 kDa, respectively.
(9 Reviews)
(36 Publications)
Species: Hu
SGPL1 Antibody
[Unconjugated]
AF5535
Western blot shows lysates of HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, HT‑29 human colon adenocarcinoma cell line, and HT‑29 tumor tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SGPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SGPL1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SGPL1 at approximately 60 kDa (as indicated) using 50 µg/mL of Goat Anti-Human SGPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5535) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
(4 Publications)
Species: Hu
Applications: WB, Simple Western
Leucyl-cystinyl
Aminopeptidase/LNPEP ...
AF6386
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Leucyl-cystinyl Aminopeptidase/LNPEP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6386) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Leucyl-cystinyl Aminopeptidase/LNPEP at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Species: Hu
Applications: WB, IP
CSRP1 Antibody
AF5739
Western blot shows lysates of ME-180 human cervical epithelial carcinoma cell line, HT-2 mouse T cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat CSRP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human CSRP1, CSRP2, CSRP3 (5 ng/lane) were included. A specific band for CSRP1 was detected at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu, Mu, Rt
Applications: WB
ACE/CD143 Antibody
[Unconjugated]
AF1513
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE/CD143 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE/CD143 at approximately 218 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
ACE/CD143 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
(1 Review)
(3 Publications)
Species: Mu
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
RFC1 Antibody
[Unconjugated]
AF6457
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc).  PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human RFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6457) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RFC1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
TNF-alpha
[Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
(24 Reviews)
(609 Publications)
Species: Hu
AGXT Antibody
NBP1-89200
Western Blot: AGXT Antibody [NBP1-89200] - Analysis in human liver tissue.
Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200] - Analysis in human liver and pancreas tissues. Corresponding AGXT RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-P
ATP6V0A1 Antibody
NBP1-89342
Immunocytochemistry/Immunofluorescence: ATP6V0A1 Antibody [NBP1-89342] - Staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ATP6V0A1 Antibody [NBP1-89342] - Staining of human tonsil shows low expression as expected.
Immunohistochemistry-Paraffin: ATP6V0A1 Antibody [NBP1-89342] - Staining of human cerebral cortex shows high expression.
(3 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
IL-6 [Unconjugated]
7270-IL/CF
IL-6 [Unconjugated]
Species: Hu
RFC2 Antibody
(OTI2B2)
NBP2-45570
Western Blot: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.
Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Adenocarcinoma of Human endometrium tissue.
Flow Cytometry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by RFC2 antibody.
Species: Hu
Applications: WB, Flow, IHC
RFC4 Antibody
(OTI1A8)
NBP2-45946
Western Blot: RFC4 Antibody (1A8) [NBP2-45946] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4.
Immunohistochemistry: RFC4 Antibody (1A8) [NBP2-45946] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: RFC4 Antibody (1A8) [NBP2-45946] - Analysis of extracts (35ug) from 9 different cell lines by using RFC4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, IHC, IF
BRD4 Antibody [HRP]
NBP1-18874H
BRD4 Antibody [HRP]
Species: Hu, Mu
Applications: WB, IHC, IHC-P

Publications for BRD4 Antibody (NBP1-18874H) (0)

There are no publications for BRD4 Antibody (NBP1-18874H).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for BRD4 Antibody (NBP1-18874H) (0)

There are no reviews for BRD4 Antibody (NBP1-18874H). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BRD4 Antibody (NBP1-18874H) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

BRD4 Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-18874AF350
Alexa Fluor 405 NBP1-18874AF405
Alexa Fluor 488 NBP1-18874AF488
Alexa Fluor 532 NBP1-18874AF532
Alexa Fluor 594 NBP1-18874AF594
Alexa Fluor 647 NBP1-18874AF647
Alexa Fluor 700 NBP1-18874AF700
Alexa Fluor 750 NBP1-18874AF750
DyLight 350 NBP1-18874UV
DyLight 405 NBP1-18874V
DyLight 488 NBP1-18874G
DyLight 550 NBP1-18874R
DyLight 594 NBP1-18874DL594
DyLight 650 NBP1-18874C
DyLight 680 NBP1-18874FR
DyLight 755 NBP1-18874IR
FITC NBP1-18874F
HRP NBP1-18874H
Janelia Fluor 549 NBP1-18874JF549
Janelia Fluor 646 NBP1-18874JF646
Learn about Custom Antibody Labeling

Additional BRD4 Products

Bioinformatics Tool for BRD4 Antibody (NBP1-18874H)

Discover related pathways, diseases and genes to BRD4 Antibody (NBP1-18874H). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BRD4 Antibody (NBP1-18874H)

Discover more about diseases related to BRD4 Antibody (NBP1-18874H).
 

Pathways for BRD4 Antibody (NBP1-18874H)

View related products by pathway.

PTMs for BRD4 Antibody (NBP1-18874H)

Learn more about PTMs related to BRD4 Antibody (NBP1-18874H).

Blogs on BRD4.

Check out the latest blog posts on BRD4.

  Read full blog post.
Understanding the relationship between NUT and BET proteins in NMC
NUT has been found to fuse with bromodomain-containing proteins 3 and 4 (BRD3 and BRD4) in NUT midline carcinoma (NMC), a very rare, extremely aggressive, and genetically defined human cancer. NMC has recently been designated as a sub classificati...  Read full blog post.
NUT - A Protein Coding Gene
The NUT gene is found on chromosome 15q14 and encodes for the NUT protein which is a key component of the RNA polymerase II Mediator complex. This multi-subunit assembly is required for all RNA pol II-dependent transcriptional activation, coordinating...  Read full blog post.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our BRD4 Antibody [HRP] and receive a gift card or discount.


Bioinformatics

Gene Symbol BRD4
Entrez
Uniprot