Beta Ig-h3/TGFBI Antibody

Images

 
Western Blot: Beta Ig-h3/TGFBI Antibody [NBP1-47215] - Staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody [NBP1-47215] - Staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Beta Ig-h3/TGFBI Antibody Summary

Immunogen
Peptide with sequence C-QLYTDRTEKLRPE corresponding to internal region according to NP_000349.1.
Epitope
Internal region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TGFBI
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2-0.5 ug/ml
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4-6 ug/ml
  • Peptide ELISA detection limit 1:32000
Application Notes
WB: Approx 70 kDa band observed in human kidney lysates (calculated MW of 74.7 kDa according to Human NP_000349.1). IHC: Paraffin embedded kidney shows staining of intercalated cells of a collecting tubule.
Publications
Read Publication using NBP1-47215.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Beta Ig-h3/TGFBI Antibody

  • beta IGH3
  • beta IG-H3
  • BIGH3EBMD
  • CDB1
  • CDG2
  • CDGG1transforming growth factor, beta-induced, 68kD
  • CSD
  • CSD1
  • CSD2
  • CSD3
  • Kerato-epithelin
  • LCD1
  • RGD-CAP
  • RGD-containing collagen-associated protein
  • TGFBI
  • TGFBIP
  • transforming growth factor, beta-induced, 68kDa
  • transforming growth factor-beta-induced protein ig-h3

Background

TGFBI, also known as BIGH3 and betaig-h3, is an extracellular matrix protein induced by transforming growth factor (TGF)-beta 1. TGFBI protein is involved in cell growth, cell differentiation, wound healing and cell adhesion. In addition, some missense mutations of TGFBI were identified in families affected with human autosomal dominant corneal dystrophies. BIGH3 gene encodes for a 683 amino-acid protein containing an RGD motif and four internal repeated domains which have highly conserved sequences founded in several species (Fasciclin domain). Clone k1H12, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TGFBI protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Beta Ig-h3/TGFBI Antibody (NBP1-47215)(1)

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Video Protocols

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Bioinformatics

Gene Symbol TGFBI
Entrez
Uniprot