beta-Catenin Antibody (12F7)

Images

 
Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and ...read more
Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (12F7) [NBP1-54467] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against ...read more
Immunohistochemistry-Paraffin: beta-Catenin Antibody (12F7) [NBP1-54467] - IHC analysis of beta- Catenin in mouse intestine using DAB with hematoxylin counterstain.
Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Western blot analysis of beta Catenin expression in 1) HepG2, 2) MCF7, and 3) Cos7 whole cell lysates using NBP1-54467.
Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - analysis of beta- Catenin in embryonic lung (lane 1) and embryonic limb (lane 2) lysates using anti-beta- Catenin antibody. Each lane was loaded with 5ug of ...read more
Immunohistochemistry-Paraffin: beta-Catenin Antibody (12F7) [NBP1-54467] - IHC analysis of beta Catenin in mouse intestine using DAB with hematoxylin counterstain.
Simple Western: beta-Catenin Antibody (12F7) [NBP1-54467] - Simple Western lane view shows a specific band for Beta- Catenin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clone
12F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

beta-Catenin Antibody (12F7) Summary

Immunogen
Recombinant chicken beta Catenin fused to maltose binding protein. [UniProt# O42486]
Localization
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole.
Marker
Epithelial Cell Marker, Adherens Junction Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CTNNB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
This beta Catenin (12F7) antibody is useful for Immunohistochemistry on paraffin embedded sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot, where a band can be seen at approx. 92 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4
using
NBP1-54467 in the following applications:

Publications
Read Publications using
NBP1-54467 in the following applications:

  • 3 publications
  • IP
    1 publication
  • WB
    6 publications

Reactivity Notes

Human, mouse, rat, monkey and chicken.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for beta-Catenin Antibody (12F7)

  • bCatenin
  • beta 1 (88kD)
  • beta-Catenin
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin beta-1
  • CTNNB
  • CTNNB1
  • DKFZp686D02253
  • FLJ25606
  • FLJ37923

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-Catenin Antibody (NBP1-54467)(6)

We have publications tested in 2 confirmed species: Human, Chicken.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(3)
IP
(1)
WB
(6)
All Applications
Filter By Species
Human
(5)
Chicken
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using NBP1-54467 Applications Species
Wu JH, Liu JH, Ko YC et al. Haploinsufficiency of RCBTB1 is associated with Coats disease and familial exudative vitreoretinopathy Hum. Mol. Genet. Feb 11 2016 [PMID: 26908610] (WB, ICC/IF, Human) WB, ICC/IF Human
Moura Rute Silva, Carvalho-Correia Eduarda, daMota Paulo, Correia-Pinto Jorge. Canonical Wnt signaling activity in early stages of chick lung development. PLoS One. 2014 Dec 02 [PMID: 25460002] (WB, Chicken) WB Chicken
Nieset JE, Redfield AR, Jin F, Knudsen KA, Johnson KR, Wheelock MJ. Characterization of the interactions of alpha-catenin with alpha-actinin and beta-catenin/plakoglobin. J Cell Sci;110 ( Pt 8):1013-22. 1997 Apr. [PMID: 9152027] (WB, ICC/IF, Human) WB, ICC/IF Human
Islam S, Carey TE, Wolf GT, Wheelock MJ, Johnson KR. Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion. J Cell Biol;135(6 Pt 1):1643-54. 1996 Dec. [PMID: 8978829] (WB, Human) WB Human
Knudsen KA, Soler AP, Johnson KR, Wheelock MJ. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin. J Cell Biol;130(1):67-77. 1995 Jul. [PMID: 7790378] (WB, IP, ICC/IF, Human) WB, IP, ICC/IF Human
Sacco PA, McGranahan TM, Wheelock MJ, Johnson KR. Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin. J Biol Chem;270(34):20201-6. 1995 Aug 25. [PMID: 7650039] (WB, Human) WB Human

Reviews for beta-Catenin Antibody (NBP1-54467) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Mouse, Other.
We have 3 reviews tested in 2 application: WB, IHC.

Reviews using NBP1-54467:
Filter by Applications
WB
(2)
IHC
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot beta-Catenin NBP1-54467
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reviewed by:
Anonymous
WB Human 07/12/2017
View

Summary

ApplicationWestern Blot
Sample Testedembryonic kidney cells,HEK293 Cells
SpeciesHuman
Western Blot beta-Catenin NBP1-54467
Enlarge
reviewed by:
Rute Moura
WB Other 10/24/2014
View

Summary

ApplicationWestern Blot
Sample Tested1 - Embryonic Lung; 2 - Embryonic limb
SpeciesOther

Blocking

Blocking Details5% TBS-T milk

Primary Anitbody

Dilution Ratio1-30000

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP-linked Antibody
Secondary Manufacturer Cat#Cell Signaling Technology Inc: 7074
Secondary Concentration1-2000

Details

Detection NotesChemiluminescence, Super Signal West Femto Substrate (Pierce Biotechnology); Chemidoc XRS (Bio-Rad); Limb protein: positive C
Immunohistochemistry beta-Catenin NBP1-54467
Enlarge
reviewed by:
Anony- mous
IHC Mouse 03/18/2014
View

Summary

ApplicationImmunohistochemistry
SpeciesMouse
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Catenin Antibody (NBP1-54467) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for beta-Catenin Antibody (NBP1-54467)

Discover related pathways, diseases and genes to beta-Catenin Antibody (NBP1-54467). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on beta-Catenin.

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Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Notch1 - A multifunctional transmembrane receptor
Notch1 is a member of the Notch family of Type 1 single-pass transmembrane proteins that share an extracellular domain of multiple epidermal growth factor-like (EGF) repeats. Notch family members play key roles in a variety of developmental process...  Read full blog post.

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Recent Reviews

4
5
1
4
1
3
1
2
0
1
0

Anonymous
07/12/2017
Application: WB
Species: Human

Rute Moura
10/24/2014
Application: WB
Species: Other

Anony- mous
03/18/2014
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol CTNNB1
Entrez
Uniprot