beta-Catenin Antibody (12F7)

Images

 
Biological Strategies: Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, ...read more
Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Activity of Wnt/beta-catenin pathway in the embryonic chick lung. Western blot analysis of active and total beta-catenin in stage b1, b2 and b3 lungs, and stage ...read more
Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (12F7) [NBP1-54467] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against ...read more
Immunohistochemistry: beta-Catenin Antibody (12F7) [NBP1-54467] - Beta-catening (green) was detected in human skin (psoriasis) using beta-catenin-FITC antibody (1:40) in PBS for 1 hour. Nuclei were stained with Dapi ...read more
Flow Cytometry: beta-Catenin Antibody (12F7) [NBP1-54467] - An intracellular stain was performed on SK-MEL-28 cells with beta-Catenin Antibody [12F7] NBP1-54467B (blue) and a matched isotype control (orange). Both ...read more
Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta Catenin expression in 1) HepG2, 2) MCF7, and 3) Cos7 whole cell lysates using NBP1-54467.
Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta- Catenin in embryonic lung (lane 1) and embryonic limb (lane 2) lysates using anti-beta- Catenin antibody. Each lane was loaded with 5ug of ...read more
Immunohistochemistry-Paraffin: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta Catenin in mouse intestine using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta- Catenin in mouse intestine using DAB with hematoxylin counterstain.
Immunohistochemistry: beta-Catenin Antibody (12F7) [NBP1-54467] - IF beta-Catenin staining of mouse brain tissue. This image submitted by a verified customer review.
Flow (Intracellular): beta-Catenin Antibody (12F7) [NBP1-54467] - An intracellular stain was performed on HeLa cells with beta-Catenin Antibody (12F7) NBP1-54467AF647 (blue) and a matched isotype control (orange). Cells ...read more
Simple Western: beta-Catenin Antibody (12F7) [NBP1-54467] - Simple Western lane view shows a specific band for Beta- Catenin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, PmSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP
Clone
12F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

beta-Catenin Antibody (12F7) Summary

Immunogen
Recombinant chicken beta Catenin fused to maltose binding protein. [UniProt# O42486]
Localization
Nucleus, Cytoplasm, Cytoskeleton, Adherens junction, Centrosome, Spindle pole.
Marker
Epithelial Cell Marker, Adherens Junction Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CTNNB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Immunoprecipitation 1:10-1:500
  • Flow (Intracellular)
Application Notes
This beta Catenin (12F7) antibody is useful for IHC-P sections, ICC/IF, IP and WB, where a band can be seen at approx. 92 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
beta-Catenin Knockout HeLa Cell Lysate
Reviewed Applications
Read 4 Reviews rated 4
using
NBP1-54467 in the following applications:

Publications
Read Publications using
NBP1-54467 in the following applications:

  • ICC
    1 publication
  • 3 publications
  • IP
    1 publication
  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for beta-Catenin Antibody (12F7)

  • bCatenin
  • beta 1 (88kD)
  • beta-Catenin
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin beta-1
  • CTNNB
  • CTNNB1
  • DKFZp686D02253
  • FLJ25606
  • FLJ37923

Background

The beta-catenin protein functions in both regulation and coordination of cell-cell adhesion and gene transcription. In humans, beta-catenin the CTNNB1 gene. beta-catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to gamma-catenin, also known as plakoglobin. Beta-catenin is widely expressed in many tissues. In cardiac muscle, beta-catenin localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP

Publications for beta-Catenin Antibody (NBP1-54467)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 4 applications: ICC, ICC/IF, IP, WB.


Filter By Application
ICC
(1)
ICC/IF
(3)
IP
(1)
WB
(6)
All Applications
Filter By Species
Human
(7)
Mouse
(1)
Chicken
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NBP1-54467 Applications Species
Bullock WA, Hoggatt A, Horan DJ et al. Expression of a degradation-resistant beta-catenin mutant in osteocytes protects the skeleton from mechanodeprivation-induced bone wasting J. Bone Miner. Res. Jun 7 2019 [PMID: 31173667] (Mouse) Mouse
Chuang CH, Wang LY, Wong YM, Lin ES. Anti-metastatic effects of isolinderalactone via the inhibition of MMP-2 and up regulation of NM23-H1 expression in human lung cancer A549 cells. Oncol Lett 2018 Apr [PMID: 29541242] (Human) Human
Farjood Farhad, Vargis Elizabeth Physical disruption of cell-cell contact induces VEGF expression in RPE cells Physical disruption of cell-cell contact induces VEGF expression in RPE cells. Mol Vis. 2017 Jul 17 [PMID: 28761317] (ICC, Human)

Details:
Citation using the DyLight 680 version of this antibody.
ICC Human
Wu JH, Liu JH, Ko YC et al. Haploinsufficiency of RCBTB1 is associated with Coats disease and familial exudative vitreoretinopathy Hum. Mol. Genet. Feb 11 2016 [PMID: 26908610] (WB, ICC/IF, Human) WB, ICC/IF Human
Moura Rute Silva, Carvalho-Correia Eduarda, daMota Paulo, Correia-Pinto Jorge. Canonical Wnt signaling activity in early stages of chick lung development. PLoS One. 2014 Dec 02 [PMID: 25460002] (WB, Chicken) WB Chicken
Nieset JE, Redfield AR, Jin F, Knudsen KA, Johnson KR, Wheelock MJ. Characterization of the interactions of alpha-catenin with alpha-actinin and beta-catenin/plakoglobin. J Cell Sci;110 ( Pt 8):1013-22. 1997 Apr. [PMID: 9152027] (WB, ICC/IF, Human) WB, ICC/IF Human
Islam S, Carey TE, Wolf GT, Wheelock MJ, Johnson KR. Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion. J Cell Biol;135(6 Pt 1):1643-54. 1996 Dec. [PMID: 8978829] (WB, Human) WB Human
Knudsen KA, Soler AP, Johnson KR, Wheelock MJ. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin. J Cell Biol;130(1):67-77. 1995 Jul. [PMID: 7790378] (WB, IP, ICC/IF, Human) WB, IP, ICC/IF Human
Sacco PA, McGranahan TM, Wheelock MJ, Johnson KR. Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin. J Biol Chem;270(34):20201-6. 1995 Aug 25. [PMID: 7650039] (WB, Human) WB Human

Reviews for beta-Catenin Antibody (NBP1-54467) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 4 species: Human, Mouse, Other, recombinant beta-catenin and N/A.

Reviews using NBP1-54467:
Filter by Applications
WB
(3)
IHC
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Other
(1)
recombinant beta-catenin and N/A
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot beta-Catenin NBP1-54467
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reviewed by:
Anonymous
WB recombinant beta-catenin and N/A 05/08/2020
View

Summary

ApplicationWestern Blot
Sample Testedrecombinant beta-catenin,beta catenin truncate (novus bio),random antibody (- control) with Alexa 647
Speciesrecombinant beta-catenin and N/A
LotN/A

Comments

Comments1:1000 antibody dilution for incubation (overnight, 4C)
blocked with 2% TBST
Western Blot beta-Catenin NBP1-54467
Enlarge
reviewed by:
Anonymous
WB Human 07/12/2017
View

Summary

ApplicationWestern Blot
Sample Testedembryonic kidney cells,HEK293 Cells
SpeciesHuman
Western Blot beta-Catenin NBP1-54467
Enlarge
reviewed by:
Rute Moura
WB Other 10/24/2014
View

Summary

ApplicationWestern Blot
Sample Tested1 - Embryonic Lung; 2 - Embryonic limb
SpeciesOther

Blocking

Blocking Details5% TBS-T milk

Primary Anitbody

Dilution Ratio1-30000

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP-linked Antibody
Secondary Manufacturer Cat#Cell Signaling Technology Inc: 7074
Secondary Concentration1-2000

Details

Detection NotesChemiluminescence, Super Signal West Femto Substrate (Pierce Biotechnology); Chemidoc XRS (Bio-Rad); Limb protein: positive C
Immunohistochemistry beta-Catenin NBP1-54467
Enlarge
reviewed by:
Anony- mous
IHC Mouse 03/18/2014
View

Summary

ApplicationImmunohistochemistry
SpeciesMouse
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Catenin Antibody (NBP1-54467). (Showing 1 - 1 of 1 FAQs).

  1. Hello, I was wondering if the NBP1-54467SS antibody is for global B-catenin, or only for phosphorylated version
    • This monoclonal Ab should detect both phosphorylated and unphosphorylated beta-Catenin.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-54467AF350
Alexa Fluor 405 NBP1-54467AF405
Alexa Fluor 488 NBP1-54467AF488
Alexa Fluor 532 NBP1-54467AF532
Alexa Fluor 594 NBP1-54467AF594
Alexa Fluor 647 NBP1-54467AF647
Alexa Fluor 700 NBP1-54467AF700
Alexa Fluor 750 NBP1-54467AF750
Biotin NBP1-54467B
DyLight 350 NBP1-54467UV
DyLight 405 NBP1-54467V
DyLight 488 NBP1-54467G
DyLight 550 NBP1-54467R
DyLight 594 NBP1-54467DL594
DyLight 650 NBP1-54467C
DyLight 680 NBP1-54467FR
DyLight 755 NBP1-54467IR
FITC NBP1-54467F
HRP NBP1-54467H
Janelia Fluor 549 NBP1-54467JF549
Janelia Fluor 646 NBP1-54467JF646

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Bioinformatics Tool for beta-Catenin Antibody (NBP1-54467)

Discover related pathways, diseases and genes to beta-Catenin Antibody (NBP1-54467). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on beta-Catenin.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

The role of Wnts in neuroinflammation
By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s...  Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Notch1 - A multifunctional transmembrane receptor
Notch1 is a member of the Notch family of Type 1 single-pass transmembrane proteins that share an extracellular domain of multiple epidermal growth factor-like (EGF) repeats. Notch family members play key roles in a variety of developmental process...  Read full blog post.

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Recent Reviews

4
5
1
4
2
3
1
2
0
1
0

Anonymous
05/08/2020
Application: WB
Species: recombinant beta-catenin and N/A

Anonymous
07/12/2017
Application: WB
Species: Human

Rute Moura
10/24/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol CTNNB1
Entrez
Uniprot