TCF4 Antibody (1G4) - Azide and BSA Free

Western Blot: TCF4 Antibody (1G4) [H00006925-M03] - Analysis of TCF4 expression in K-562 (Cat # L009V1).

Immunocytochemistry/Immunofluorescence: TCF4 Antibody (1G4) [H00006925-M03] - Analysis of monoclonal antibody to TCF4 on HeLa cell. Antibody concentration 10 ug/ml

ELISA: TCF4 Antibody (1G4) [H00006925-M03] - Detection limit for recombinant GST tagged TCF4 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt, Pm, Rb
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 1G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TCF4 Antibody (1G4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TCF4 (AAH31056.1, 1 a.a. ~ 365 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKNGPTSLASGHFTGSNVEDRSSSGSWGNGGHPSPSRNYGDGTPYDHTTSRDLGSHDNLSPPFVNSRIQSKTERGSYSSYGRESNLQGCHQQSLLGGDMDMGNPGTLSPTKPGSQYYQYSSNNPRRRPLHSSAMEVQTKKVRKVPPGLPSSVYAPSASTADYNRDSPGYPSSKPATSTFPSSFFMQDGHHSSDPWSSSSGMNQPGYAGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMSTFHRSGTNHYSTSSCTPPANGTDSIMANRGSGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGSPPSLSAGTAVWSRN
• Specificity: TCF4 (1G4)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TCF4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: This antibody reactivity against cell lysate and recombinant protein for WB. It has been useful for IF and ELISA.

Reactivity Notes

Rat reactivity reported in customer reivew. Rabbit reactivity reported in scientific literature (PMID: 29940337).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TCF4 Antibody (1G4) - Azide and BSA Free

• BHLHB19
• bHLHb19E2-2
• Class B basic helix-loop-helix protein 19
• Immunoglobulin transcription factor 2
• ITF-2
• ITF2PTHS
• MGC149724
• SEF-2
• SEF2-1
• SEF2-1A
• SEF2-1B
• SEF2MGC149723
• SL3-3 enhancer factor 2
• TCF-4
• transcription factor 4

Background

This gene encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCF4 Antibody (H00006925-M03)(8)

Publications using H00006925-M03

• Simone M, Reinier B, Marten S et al. Tcf4 Encodes Correct Brain Development During Embryogenesis. Mol Cell Neurosci. 2020-05-28 [PMID: 32474139]
• Huang P, Zhou Q, Lin Q, Lin L et Al. Complement C3a induces axonal hypomyelination in the periventricular white matter through activation of WNT/?-catenin signal pathway in septic neonatal rats experimentally induced by lipopolysaccharide Brain Pathol 2019-10-18 [PMID: 31622511]
• Dohare P, Cheng B, Ahmed E et al. Glycogen synthase kinase-3b inhibition enhances myelination in preterm newborns with intraventricular hemorrhage, but not recombinant Wnt3A Neurobiol. Dis. 2018-06-22 [PMID: 29940337] (Rabbit)
• Xia H, Jahr FM, Kim NK et al. Building a schizophrenia genetic network: Transcription Factor 4 regulates genes involved in neuronal development and schizophrenia risk Hum. Mol. Genet. 2018-06-14 [PMID: 29905862] (Human)
• Ruiz de Garibay G, Mateo F, Stradella A et al. Tumor xenograft modeling identifies an association between TCF4 loss and breast cancer chemoresistance. Dis Model Mech 2018-05-18 [PMID: 29666142]
• Luo C, Lim JH, Lee Y et al. A PGC1alpha-mediated transcriptional axis suppresses melanoma metastasis. Nature 2016-08-31 [PMID: 27580028]
• Forrest MP, Waite AJ, Martin-Rendon E, Blake DJ. Knockdown of Human TCF4 Affects Multiple Signaling Pathways Involved in Cell Survival, Epithelial to Mesenchymal Transition and Neuronal Differentiation PLoS One. 2013-08-23 [PMID: 24058414]
• Tanaka A, Itoh F, Nishiyama K. Inhibition of endothelial cell activation by bHLH protein E2-2 and its impairment of angiogenesis. Blood 115(20):4138-47. 2010-05-20 [PMID: 20231428]

Review for TCF4 Antibody (H00006925-M03) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer WB Other 11/05/2009

Summary

• Application: Western Blot
• Sample Tested: breast cancer cell line, Sample Amount: 40
• Species: Other
• Lot: 7152-1G4-00Ka6

Bioinformatics

• Gene Symbol: TCF4
• Entrez:
  • 6925(Human)
• OMIM:
  • 602272(Human)
• Uniprot:
  • BC031056(Human)