TCF4 Antibody (1G4)

Images

 
Western Blot: TCF4 Antibody (1G4) [H00006925-M03] - Analysis of TCF4 expression in K-562 (Cat # L009V1).
Immunocytochemistry/ Immunofluorescence: TCF4 Antibody (1G4) [H00006925-M03] - Analysis of monoclonal antibody to TCF4 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: TCF4 Antibody (1G4) [H00006925-M03] - Detection limit for recombinant GST tagged TCF4 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
1G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

TCF4 Antibody (1G4) Summary

Immunogen
TCF4 (AAH31056.1, 1 a.a. - 365 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKNGPTSLASGHFTGSNVEDRSSSGSWGNGGHPSPSRNYGDGTPYDHTTSRDLGSHDNLSPPFVNSRIQSKTERGSYSSYGRESNLQGCHQQSLLGGDMDMGNPGTLSPTKPGSQYYQYSSNNPRRRPLHSSAMEVQTKKVRKVPPGLPSSVYAPSASTADYNRDSPGYPSSKPATSTFPSSFFMQDGHHSSDPWSSSSGMNQPGYAGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMSTFHRSGTNHYSTSSCTPPANGTDSIMANRGSGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGSPPSLSAGTAVWSRN
Specificity
TCF4 (1G4)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TCF4
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been useful for IF and ELISA.
Reviewed Applications
Read 1 Review rated 4
using
H00006925-M03 in the following applications:

Publications
Read Publications using H00006925-M03.

Reactivity Notes

Rat reactivity reported in customer reivew.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TCF4 Antibody (1G4)

  • BHLHB19
  • bHLHb19E2-2
  • Class B basic helix-loop-helix protein 19
  • Immunoglobulin transcription factor 2
  • ITF-2
  • ITF2PTHS
  • MGC149724
  • SEF-2
  • SEF2-1
  • SEF2-1A
  • SEF2-1B
  • SEF2MGC149723
  • SL3-3 enhancer factor 2
  • TCF-4
  • transcription factor 4

Background

This gene encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TCF4 Antibody (H00006925-M03)(3)

Review for TCF4 Antibody (H00006925-M03) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using H00006925-M03:
Filter by Applications
WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
Western Blot TCF4 H00006925-M03
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reviewed by:
Anonymous
WB Other 11/05/2009
View

Summary

ApplicationWestern Blot
Sample Testedbreast cancer cell line, Sample Amount: 40
SpeciesOther
Lot7152-1G4-00Ka6

Blocking

Blocking DetailsBlocking Buffer: 5% milk, Blocking Time: 1 hour, Blocking Temp: 22 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1/500, Dilution Concentration: 1 mg/ml, Incubation Dilution Buffer: 5% milk, Incubation Time/Temp: 1 hour at 22C

Secondary Antibody

Secondary DescriptionSecondary Ab: Cell signalling Mouse pAb, conj. HRP, Secondary Ab Dilution Ratio: 1/20000

Details

Detection NotesDetection Method: ECL, Exposure Time: 2 minutes, Specific Bands: 80 kDa, Non-Specific Bands: 66, 36, 100 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TCF4 Antibody (H00006925-M03) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TCF4 Antibody (H00006925-M03)

Discover related pathways, diseases and genes to TCF4 Antibody (H00006925-M03). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on TCF4.

Beta Catenin in Cell Adhesion and T-cell Signaling
Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly associates with cell surface cadherin glycoproteins. It is one member of the catenin family that includes alpha Catenin, beta Catenin, and gamma Catenin. Colocalization studies usi...  Read full blog post.

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Recent Reviews

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4
1
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1
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Anonymous
11/05/2009
Application: WB
Species: Other

Bioinformatics

Gene Symbol TCF4
Entrez
OMIM
Uniprot