Axin-1 Antibody

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Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Axin-1 Antibody Summary

Immunogen
E. coli-derived recombinant human Axin-1
Tyr210-Glu410
Accession # O15169
Specificity
Detects human, mouse and rat Axin-1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AXIN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF3287 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Axin-1 Antibody

  • axin 1
  • Axin1
  • Axin-1
  • AXINaxis inhibitor 1
  • Axis inhibition protein 1
  • fused, mouse, homolog of
  • hAxin
  • MGC52315

Background

Axin-1 is a negative regulator of the Wnt signaling pathway, which is involved in regulation of embryonic axis formation in vertebrates. Axin-1 binds to beta -catenin and glycogen synthase kinase-3 (GSK-3) to promote beta -catenin phosphorylation and subsequent degradation. Axin-1 also binds to the adenomatous polyposis coli (APC) gene, a known tumor suppressor that also regulates beta -catenin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Axin-1 Antibody (AF3287)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


Filter By Application
Immunoprecipitation
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF3287 Applications Species
C Dittfeld, G Reimann, A Mieting, P Büttner, A Jannasch, K Plötze, G Steiner, SM Tugtekin, K Matschke Treatment with XAV-939 prevents in vitro calcification of human valvular interstitial cells PLoS ONE, 2018;13(12):e0208774. 2018 [PMID: 30532256] (Western Blot, Human) Western Blot Human
K Saito-Diaz, H Benchabane, A Tiwari, A Tian, B Li, JJ Thompson, AS Hyde, LM Sawyer, JN Jodoin, E Santos, LA Lee, RJ Coffey, RD Beauchamp, CS Williams, AK Kenworthy, DJ Robbins, Y Ahmed, E Lee APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway Dev. Cell, 2018;44(5):566-581.e8. 2018 [PMID: 29533772] (Western Blot, Mouse) Western Blot Mouse
Yu N, Kakunda M, Pham V, Lill J, Du P, Wongchenko M, Yan Y, Firestein R, Huang X HSP105 recruits protein phosphatase 2A to dephosphorylate beta-catenin. Mol Cell Biol, 2015;35(8):1390-400. 2015 [PMID: 25645927] (Immunoprecipitation, Human) Immunoprecipitation Human
Bao R, Christova T, Song S, Angers S, Yan X, Attisano L Inhibition of tankyrases induces Axin stabilization and blocks Wnt signalling in breast cancer cells. PLoS ONE, 2012;7(11):e48670. 2012 [PMID: 23144924] (Western Blot, Human) Western Blot Human
Dual functions of DP1 promote biphasic Wnt-on and Wnt-off states during anteroposterior neural patterning. EMBO J., 2012;31(16):3384-97. 2012 [PMID: 22773187] (Western Blot, Human) Western Blot Human
Steele BM, Harper MT, Macaulay IC, Morrell CN, Perez-Tamayo A, Foy M, Habas R, Poole AW, Fitzgerald DJ, Maguire PB Canonical Wnt signaling negatively regulates platelet function. Proc. Natl. Acad. Sci. U.S.A., 2009;106(47):19836-41. 2009 [PMID: 19901330] (Western Blot, Human) Western Blot Human

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Video Protocols

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Bioinformatics

Gene Symbol AXIN1
Uniprot