Axin-1 Antibody

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Axin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3287) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axin-1 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Axin-1 Antibody Summary

• Immunogen: E. coli-derived recombinant human Axin-1
  Tyr210-Glu410
  Accession # O15169
• Specificity: Detects human, mouse and rat Axin-1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AXIN1
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Axin-1 Antibody

• axin 1
• Axin1
• Axin-1
• AXINaxis inhibitor 1
• Axis inhibition protein 1
• fused, mouse, homolog of
• hAxin
• MGC52315

Background

Axin-1 is a negative regulator of the Wnt signaling pathway, which is involved in regulation of embryonic axis formation in vertebrates. Axin-1 binds to beta -catenin and glycogen synthase kinase-3 (GSK-3) to promote beta -catenin phosphorylation and subsequent degradation. Axin-1 also binds to the adenomatous polyposis coli (APC) gene, a known tumor suppressor that also regulates beta -catenin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Axin-1 Antibody (AF3287)(9)

Publications using AF3287

• W Wang, P Liu, M Lavrijsen, S Li, R Zhang, S Li, WS van de Gee, HJG van de Wer, MP Peppelenbo, R Smits Evaluation of AXIN1 and AXIN2 as targets of tankyrase inhibition in hepatocellular carcinoma cell lines Scientific Reports, 2021;11(1):7470. 2021 [PMID: 33811251] (Western Blot, Human)
• TW Parker, KL Neufeld APC controls Wnt-induced beta-catenin destruction complex recruitment in human colonocytes Sci Rep, 2020;10(1):2957. 2020 [PMID: 32076059] (Western Blot, Human)
• C Dittfeld, G Reimann, A Mieting, P Büttner, A Jannasch, K Plötze, G Steiner, SM Tugtekin, K Matschke Treatment with XAV-939 prevents in vitro calcification of human valvular interstitial cells PLoS ONE, 2018;13(12):e0208774. 2018 [PMID: 30532256] (Western Blot, Human)
• K Saito-Diaz, H Benchabane, A Tiwari, A Tian, B Li, JJ Thompson, AS Hyde, LM Sawyer, JN Jodoin, E Santos, LA Lee, RJ Coffey, RD Beauchamp, CS Williams, AK Kenworthy, DJ Robbins, Y Ahmed, E Lee APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway Dev. Cell, 2018;44(5):566-581.e8. 2018 [PMID: 29533772] (Western Blot, Mouse)
• Lebensohn A, Dubey R, Neitzel L, Tacchelly-Benites O, Yang E, Marceau C, Davis E, Patel B, Bahrami-Nejad Z, Travaglini K, Ahmed Y, Lee E, Carette J, Rohatgi R Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling. Elife, 0;5(0):. 0 [PMID: 27996937] (Western Blot, Mouse)
• Yu N, Kakunda M, Pham V, Lill J, Du P, Wongchenko M, Yan Y, Firestein R, Huang X HSP105 recruits protein phosphatase 2A to dephosphorylate beta-catenin. Mol Cell Biol, 2015;35(8):1390-400. 2015 [PMID: 25645927] (Immunoprecipitation, Human)
• Bao R, Christova T, Song S, Angers S, Yan X, Attisano L Inhibition of tankyrases induces Axin stabilization and blocks Wnt signalling in breast cancer cells. PLoS ONE, 2012;7(11):e48670. 2012 [PMID: 23144924] (Western Blot, Human)
• Dual functions of DP1 promote biphasic Wnt-on and Wnt-off states during anteroposterior neural patterning. EMBO J., 2012;31(16):3384-97. 2012 [PMID: 22773187] (Western Blot, Human)
• Steele BM, Harper MT, Macaulay IC, Morrell CN, Perez-Tamayo A, Foy M, Habas R, Poole AW, Fitzgerald DJ, Maguire PB Canonical Wnt signaling negatively regulates platelet function. Proc. Natl. Acad. Sci. U.S.A., 2009;106(47):19836-41. 2009 [PMID: 19901330] (Western Blot, Human)

Bioinformatics

• Gene Symbol: AXIN1
• Uniprot:
  • O15169()