Mouse BDNF ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Culture Supernatant, Plasma (Citrate, EDTA, Heparin), Serum
Standard Curve Range
31.2 to 2000 pg/mL
Sensitivity
2 pg/mL

Order Details

Mouse BDNF ELISA Kit (Colorimetric) Summary

Description
Bdnf (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse Bdnf.
Standard Curve Range
31.2 to 2000 pg/mL
Sensitivity
2 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 7.2%
Intra-Assay
CV% < 3.7%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
BDNF

Applications/Dilutions

Application Notes
Useful for quantification.
Publications
Read Publications using KA0331.

Packaging, Storage & Formulations

Storage
Store at -20 °C.

Kit Components

Components
  1. Sample diluent buffer
  2. TMB color developing agent
  3. TMB stop solution
  4. Avidin-Biotin-Peroxidase Complex (ABC) (dilution 1:100)
  5. Antibody diluent buffer
  6. 96-well Plate precoated with anti-mouse BDNF antibody
  7. ABC diluent buffer
  8. Adhesive cover
  9. Biotinylated anti- mouse BDNF antibody (dilution 1:100)
  10. Lyophilized recombinant mouse BDNF standard

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse BDNF ELISA Kit (Colorimetric)

  • Abrineurin
  • ANON2
  • BDNF
  • brain-derived neurotrophic factor
  • BULN2
  • MGC34632
  • neurotrophin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for BDNF ELISA Kit (KA0331)(9)


Showing Publications 1 - 9 of 9.
Publications using KA0331 Applications Species
Harris NM, Ritzel R, Mancini N et al. Nano-particle delivery of brain derived neurotrophic factor after focal cerebral ischemia reduces tissue injury and enhances behavioral recovery. Pharmacol Biochem Behav 2016 Sep 9 [PMID: 27619636]
Klaren RE, Stasula U, Steelman AJ et al. Effects of exercise in a relapsing-remitting model of experimental autoimmune encephalomyelitis. J Neurosci Res 2016 Oct [PMID: 27312674]
Dekeyster E, Geeraerts E, Buyens T et al. Tackling Glaucoma from within the Brain: An Unfortunate Interplay of BDNF and TrkB. PLoS One. 2015 Nov 11 [PMID: 26560713]
Sumida M, Hane M, Yabe U et al. Rapid trimming of cell surface polySia by exovesicular sialidase triggers release of preexisting surface neurotrophin. J Biol Chem. 2015 May 22 [PMID: 25750127]
Wu CH, Hung TH, Chen CC et al. Post-Injury Treatment with 7,8-Dihydroxyflavone, a TrkB Receptor Agonist, Protects against Experimental Traumatic Brain Injury via PI3K/Akt Signaling. PLoS One. 2014 Nov 21 [PMID: 25415296]
Nuytens K, Gantois I, Stijnen P et al. Haploinsufficiency of the autism candidate gene Neurobeachin induces autism-like behaviors and affects cellular and molecular processes of synaptic plasticity in mice. Neurobiol Dis. 2012 Nov 12 [PMID: 23153818]
Kovalchuk Y, Hanse E, Kafitz KW, Konnerth A. Postsynaptic Induction of BDNF-Mediated Long-Term Potentiation. Science. 2002 Mar 1 [PMID: 11872844]
Lyons WE, Mamounas LA, Ricaurte GA et al. Brain-derived neurotrophic factor-deficient mice develop aggressiveness and hyperphagia in conjunction with brain serotonergic abnormalities. Proc Natl Acad Sci U S A. 1999 Dec 21 [PMID: 10611369]
Jones KR, Reichardt LF. Molecular cloning of a human gene that is a member of the nerve growth factor family. Proc Natl Acad Sci U S A. 1990 Oct [PMID: 2236018]

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Product General Protocols

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FAQs for BDNF ELISA Kit (KA0331) (0)

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Bioinformatics Tool for BDNF ELISA Kit (KA0331)

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Research Areas for BDNF ELISA Kit (KA0331)

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Blogs on BDNF.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

TrkB and Nervous System Function
Neutrophins and their receptors play an important role in regulating the development of both the central and peripheral nervous systems. Neurotrophin ligand binding to each of their respective Trk cellular receptors is essential for the growth and sur...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

BDNF Antibodies Aid Research on Alzheimer's Therapies
Brain-derived neurotrophic factor (BDNF) is known to be important for neuronal differentiation, survival, migration and plasticity in both the developing embryo and adult synapses. The BDNF antibody is also proving to be an important tool in Alzheimer...  Read full blog post.

BDNF Antibodies and Synaptic Research
Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophins. During development it regulates the survival and differentiation of neuronal cell populations in the central and peripheral nervous system, while in adult synapse...  Read full blog post.

Save Time & Money with High Quality Sandwich ELISA Kits
Enzyme-linked immunosorbent assay (ELISA) is a widely used technique for detecting concentration of proteins, using enzyme tagged antibodies with which react with dyes to produce a colorimetric or fluorescent signal. Sandwich ELISA takes this one step...  Read full blog post.

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Bioinformatics

Gene Symbol BDNF
Entrez