BDNF Antibody (14C8.1D9)

Product Discontinued

BDNF Antibody (14C8.1D9) Summary

• Immunogen: Partial recombinant human BDNF protein (between amino acids 100-247) [UniProt P23560]
• Localization: Secreted, Cytoplasm
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BDNF
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 50 ug/ml
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10-15 ug/ml
  • Immunohistochemistry-Paraffin 10-15 ug/ml
  • Western Blot 2 ug/ml
• Application Notes: BDNF is a 247 amino acids long protein with predicted molecular weight of 27.8 kDa, however, it undergoes molecular processing / cleavage as well as glycosylation and disulphide bond formation. Cleaved BDNF is ~14 kDa while the glycosylated form as well as the dimers may run at relatively higher molecular weight position in WB assay.

Reactivity Notes

Immunogen has 100% homology with mouse, rat, bovine, porcine, and monkey. Rat data from customer review.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for BDNF Antibody (14C8.1D9)

• Abrineurin
• ANON2
• BDNF
• brain-derived neurotrophic factor
• BULN2
• MGC34632
• Neurotrophin

Background

BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycaemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Subunit: Monomers and homodimers. Binds to NTRK2/TRKB. Subcellular Location: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). Similarity: Belongs to the NGF-beta family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BDNF Antibody (NBP2-42215)(7)

Publications using NBP2-42215

• Luo S, Su Kang S, Wang ZH et al. Akt Phosphorylates NQO1 and Triggers its Degradation, Abolishing its Antioxidative Activities in Parkinson's Disease J. Neurosci. 2019-07-29 [PMID: 31358653]
• Wang ZH, Xiang J, Liu X et al. Deficiency in BDNF/TrkB Neurotrophic Activity Stimulates delta-Secretase by Upregulating C/EBP beta in Alzheimer's Disease Cell Rep 2019-07-16 [PMID: 31315045] (WB, KD, Mouse, Human, Rat)
• Xiang, J;Wang, ZH;Ahn, EH;Liu, X;Yu, SP;Manfredsson, FP;Sandoval, IM;Ju, G;Wu, S;Ye, K; Delta-secretase-cleaved Tau antagonizes TrkB neurotrophic signalings, mediating Alzheimer's disease pathologies Proc. Natl. Acad. Sci. U.S.A. 2019-04-19 [PMID: 31004063] (ICC/IF, Mouse)
• Wang ZH, Wu W, Kang SS et al. BDNF inhibits neurodegenerative disease-associated asparaginyl endopeptidase activity via phosphorylation by AKT JCI Insight 2018-08-23 [PMID: 30135302] (ICC/IF, Rat)
• Lambert WS, Carlson BJ, Formichella CR et al. Oral Delivery of a Synthetic Sterol Reduces Axonopathy and Inflammation in a Rodent Model of Glaucoma. Front Neurosci. 2017-02-22 [PMID: 28223915] (IF/IHC, Rat)
• Wei X, Ma W, Gu H et al. Intra-amniotic mesenchymal stem cell therapy improves the amniotic fluid microenvironment in rat spina bifida aperta fetuses Cell proliferation 2022-10-20 [PMID: 36266504] (IHC-Fr, Rat)

Reviews for BDNF Antibody (NBP2-42215) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Rat.

reviewed by: Verified Customer WB Rat 07/29/2017

Summary

• Application: Western Blot
• Sample Tested: rat tissue
• Species: Rat

Comments

• Comments: BDNF antibody(1 : 1000)was used in our test.Product quaility is good.

reviewed by: Verified Customer Simple Western Rat 06/15/2016

Summary

• Application: Simple Western
• Sample Tested: Rat brain
• Species: Rat

Bioinformatics

• Gene Symbol: BDNF
• Entrez:
  • 627(Human)
• Uniprot:
  • P23560()