BCR Antibody

Images

 
Independent Antibodies: Western Blot: BCR Antibody [NBP1-19098] - Whole cell lysate from HeLa and 293T cells. BCR was also immunoprecipitated by rabbit anti-BCR antibody NBP1-19097.
Immunocytochemistry/ Immunofluorescence: BCR Antibody [NBP1-19098] - Human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody used at a dilution of 1:1,000 ...read more
Flow Cytometry: BCR Antibody [NBP1-19098] - Flow Cytometric Detection of BCR. 1 x 106 K562 cells were fixed, permeabilized, and stained with 0.25 mcg anti-BCR in a 150 mcl reaction. Isotype anti-KLH control (red), no ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

BCR Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1221 and 1271 of human breakpoint cluster region using the numbering given in entry NP_004318.3 (GeneID 613).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCR
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Flow Cytometry 0.5 ug/10^6 cells in 150 ul
  • Immunocytochemistry/Immunofluorescence 1:500-1:2000
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 10 ug/mg lysate
Application Notes
For IHC: Epitope retrieval with citrate buffer pH6.0 is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NBP1-19097, NB100-77315

Alternate Names for BCR Antibody

  • ALL
  • ALLD22S11
  • BCR
  • BCR1
  • breakpoint cluster region protein
  • breakpoint cluster region
  • CMLEC 2.7.11.1
  • D22S662
  • PHL
  • PHLFLJ16453
  • Renal carcinoma antigen NY-REN-26

Background

A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol BCR
Entrez
Uniprot