BLNK Antibody (5G9) - BSA Free

Images

 
Western Blot: BLNK Antibody (5G9) [NBP2-37585] - Western blot analysis using BLNK mouse mAb against NIH/3T3 (1) and BCBL-1 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: BLNK Antibody (5G9) [NBP2-37585] - Immunofluorescence analysis of HepG2 cells using BLNK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Immunohistochemistry-Paraffin: BLNK Antibody (5G9) [NBP2-37585] - Analysis of human cervical cancer tissues using BLNK mouse mAb with DAB staining.
Flow Cytometry: BLNK Antibody (5G9) [NBP2-37585] - Flow cytometric analysis of NIH/3T3 cells using BLNK mouse mAb (green) and negative control (purple).
Western Blot: BLNK Antibody (5G9) [NBP2-37585] - Western blot analysis using BLNK mAb against human BLNK (AA: 34-216) recombinant protein. (Expected MW is 60 kDa)
ELISA: BLNK Antibody (5G9) [NBP2-37585] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
5G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

BLNK Antibody (5G9) - BSA Free Summary

Immunogen
Purified recombinant fragment of human BLNK expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BLNK
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-37585 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for BLNK Antibody (5G9) - BSA Free

  • AGM4
  • B cell linker protein
  • BASH
  • B-cell adapter containing a SH2 domain protein
  • B-cell adapter containing a Src homology 2 domain protein
  • B-cell linker protein
  • B-cell linker
  • BLNK
  • BLNK-s
  • Cytoplasmic adapter protein
  • Ly57
  • MGC111051
  • SLP65
  • SLP-65BLNK-S
  • SLP65Ly57
  • Src homology 2 domain-containing leukocyte protein of 65 kDa

Background

B-cell linker protein (BLNK, also known as SLP65, BASH, and BCA) is a B cell adaptor that plays an important role in B cell development and in BCR-signal transduction. Since BLNK does not encode any intrinsic enzymatic activity, its function is to serve as a scaffold for assembling macromolecular complexes which includes enzymes (PLC gamma, Vav and Btk) and additional linker proteins (Grb2 and Nck) (1-3). In addition, BLNK binds Btk and is required for activation of the transcription factor NF-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BLNK Antibody (NBP2-37585)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Human
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BLNK