BCR Antibody (S02-6I0)

Images

 
Western Blot: BCR Antibody (S02-6I0) [NBP3-15006] - Western blot detection of BCR in A549, HL-60, U2OS, C6 cell lysates using BCR Rabbit mAb (1:1000 diluted). Predicted band size: 143kDa. Observed band size: 160kDa.
Immunohistochemistry-Paraffin: BCR Antibody (S02-6I0) [NBP3-15006] - Immunohistochemistry of BCR in paraffin-embedded Human Brain using BCR Rabbit mAb at dilution 1/50

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
S02-6I0
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

BCR Antibody (S02-6I0) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of human BCR (Uniprot#: P11274)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
BCR
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Paraffin
  • Western Blot
Theoretical MW
160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Purity
Affinity purified

Alternate Names for BCR Antibody (S02-6I0)

  • ALL
  • ALLD22S11
  • BCR
  • BCR1
  • breakpoint cluster region protein
  • breakpoint cluster region
  • CMLEC 2.7.11.1
  • D22S662
  • PHL
  • PHLFLJ16453
  • Renal carcinoma antigen NY-REN-26

Background

The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML) (1-2). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42 (3). Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BCR